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IL-1 Beta antibody | 1D4

Mouse anti Sheep Interleukin-1 beta

Product Type
Monoclonal Antibody
Clone
1D4
Isotype
IgG1
Specificity
IL-1 Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA1658
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F * WB 0.25 mg loader
List Price Your Price
loader

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 recognizes ovine interleukin-1 beta, and shows no cross-reactivity with ovine IL-6, IL-8, TNF alpha or MCP-1.

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 demonstrates partial neutralizing activity of ovine IL-1 beta.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Bovine
Horse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Recombinant ovine IL-1 Beta
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10
Immunohistology - Frozen
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
ELISA
Mouse anti interleukin-1 beta antibody, clone 1D4 may be used as a capture antibody in a bovine IL-1 beta sandwich ELISA together with Rabbit anti Bovine interleukin-1β antibody (AHP851B) as the detection reagent for evaluation of IL-1β levels in bovine samples together with recombinant Bovine interleukin-1β (PBP008) used as standards.
Alternatively, Mouse anti Interleukin-1 beta antibody, clone 1D4 can be used as a capture reagent together with Rabbit anti Ovine interleukin-1β antibody (AHP423) as a detection reagent for the evaluation of IL-1β levels in ovine, bovine or caprine samples, again utilizing recombinant bovine IL-1&beta (PBP008) as an internal standard.

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Description Mouse IgG1 Negative Control

References for IL-1 Beta antibody

  1. Martoriati, A. & Gérard, N. (2003) Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare.
    Reprod Biol Endocrinol. 1: 42-51.
  2. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  3. Bannerman, D.D. et al. (2004) Characterization of the bovine innate immune response to intramammary infection with Klebsiella pneumoniae.
    J Dairy Sci. 87 (8): 2420-32.
  4. Leite, F. et al. (2005) Incubation of bovine PMNs with conditioned medium from BHV-1 infected peripheral blood mononuclear cells increases their susceptibility to Mannheimia haemolytica leukotoxin.
    Vet Immunol Immunopathol. 103 (3-4): 187-93.
  5. Matthews, K. et al. (2007) The effect of gene gun-delivered pGM-CSF on the immunopathology of the vaccinated skin.
    Scand J Immunol. 65 (3): 298-307.
  6. Jacobsen, S. et al. (2007) The cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle.
    Vet J. 174 (1): 170-5.
  7. Cox, R.A. et al. (2007) Production of pro-inflammatory polypeptides by airway mucous glands and its potential significance.
    Pulm Pharmacol Ther. 20 (2): 172-7.
  8. Rainard P et al. (2008) Staphylococcus aureus lipoteichoic acid triggers inflammation in the lactating bovine mammary gland.
    Vet Res. 39 (5): 52.
  9. View The Latest Product References
  10. Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
    Clin Vaccine Immunol. 17 (11): 1797-809.
  11. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  12. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  13. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  14. Karisnan K et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
    PLoS One. 10 (4): e0124390.
  15. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  16. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
    Reprod Sci. 22 (11): 1409-20.
  17. Sobotta, K. et al. (2016) Coxiella burnetii. Infects Primary Bovine Macrophages and Limits Their Host Cell Response.
    Infect Immun. 84 (6): 1722-34.
  18. Cortes, M. et al. (2017) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: A glial in vivo-in vitro multi-hit paradigm in large mammalian brain.
    J Neurosci Methods. 276: 23-32.
  19. Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  20. Cao, M. et al. (2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
    Front Immunol. 10: 1063.
  21. Stassi, A.F. et al. (2019) Follicular structures of cows with cystic ovarian disease present altered expression of cytokines.
    Zygote. 15: 1-14.
  22. Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro. sheep model.
    J Dairy Sci. Mar 17 [Epub ahead of print].
  23. Damiano, S. et al. (2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambs
    It J Anim Sci. 21 (1): 934-42.
  24. Stassi, A.F. et al. (2018) Altered expression of IL-1β, IL-1RI, IL-1RII, IL-1RA and IL-4 could contribute to anovulation and follicular persistence in cattle.
    Theriogenology. 110: 61-73.
  25. Ciliberti, M.G. et al. (2024) Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs.
    Front Vet Sci. 11: 1340141.

Further Reading

  1. Rothel, J.S. et al. (1997) Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry.
    Vet Immunol Immunopathol. 57 (3-4): 267-78.

ELISA

RRID
AB_322126
UniProt
P21621
Entrez Gene
IL-1B
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division

MCA1658

154759 155911 161097 166426 166653

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