IL-1 Beta antibody | 1D4

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Anti Sheep Interleukin-1 beta Antibody, clone 1D4 thumbnail image 1

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 thumbnail image 2

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 thumbnail image 3

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 thumbnail image 4

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 thumbnail image 5

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 gallery image 1 — **Published customer image:**
Rabbit anti Sheep interleukin 1β antibody (AHP423) used for the detection of bound interleukin 1β from lamb sera in an ELISA using Mouse anti Sheep IL-1&beta antibody, clone 1D4 as a capture antibody (**MCA1658**). For IL-6, capture is with Mouse anti Sheep IL-6 antibody, clone 4B6 (MCA1659) and detection using Rabbit anti Sheep IL-6 antibody (AHP424). Subsequent visualisation uses HRP conjugated Goat anti Rabbit IgG in both cases.
**Image caption:**
Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS.

From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015)
Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS ONE 10(4): e0124390.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 gallery image 2 — **Published customer image:**
Mouse anti Ovine IL-1β antibody, clone 1D4 (**MCA1658**) used for the determination of IL-1β signal in equine follicular fluid samples following gonadotrophin administration by western blotting.
**Image caption:**
Content of IL-1β in follicular fluid at different stages: early dominance [ED], end of growth [T0 h], 6 h [T6 h], 12 h [T12 h], 24 h [T24 h] and 34 h [T34 h] after injection of crude equine gonadotropin A) Western immunoblots using a mouse anti-ovine IL-1β. Lane 1, early dominance [ED]; lane 2, end of growth [T0 h]; lanes 3, 4, 5 and 6, 6 h [T6 h], 12 h [T12 h], 24 h [T24 h], and 34 h [T34 h] after CEG injection respectively. Lanes P1 and P2, positive controls (ovine follicular fluids). B) Quantification of IL-1β signals visualized in follicular fluid at different stages as described above. IL-1β signal in each lane was measured by ImageQuant software and is expressed per follicle. Results are expressed in arbitrary units and represent means ± SEM of four to seven samples. a, b, and c are significantly different (p < 0.05 at least) according to the non parametric Wilcoxon Mann Whitney test. The data presented are representative of four experiments (electrophoresis and immunoblotting).

From: Martoriati A, Gérard N.
Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare.
Reprod Biol Endocrinol. 2003 May 16;1:42.
Copyright © 2003 Martoriati and Gérard; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 gallery image 3 — **Published customer image:**
Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1β antibody (**MCA1658**) as a capture reagent
**Image caption:**
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental *S. epidermidis* (-○-) and *S. simulans* (-●-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with *S. simulans* than with *S. epidermidis*. The SCC is expressed as a natural logarithm of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with *Staphylococcus simulans* and *S. epidermidis*.
Vet Res. 2011 Mar 17;42:49.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 gallery image 4 — **Published customer image:**
Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1&beta antibody (**MCA1658**) as a capture reagent
**Image caption:**
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-□-) and spontaneously eliminated (-■-) *S. epidermidis* and *S. simulans* intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with *Staphylococcus simulans* and *S. epidermidis*.
Vet Res. 2011 Mar 17;42:49.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Sheep Interleukin-1 beta Antibody, clone 1D4 gallery image 5 — **Published customer image:**
Mouse anti Ovine interleukin 1β antibody, clone 1D4 (**MCA1658**) used as a capture reagent in conjunction with Rabbit anti Ovine interleukin 1β antibody (**AHP423**) as a detection antibody in a sandwich ELISA for the evaluation of IL-1β secretion in ovine samples.
**Image caption:**
(Left) IL-1β secretion in ovine primary astrocyte cultures in response to 6 h LPS exposure without or with pre-incubation with α7nAChR antagonist (B100) or agonist (A10) for 1 h. Single hit, *in vitro* only LPS exposure; second hit, *in vivo* systemic and subsequent *in vitro* LPS exposure 4 to 5 weeks later. Y axis shows fold changes in IL-1β in relation to the levels of sham treatment (LPS). Generalized estimating equations (GEE) modeling results are presented in text and no significance marks are provided in the figure. For both box plots, an asterisk represents an extreme outlier (a value more than 3 times the interquartile range from a quartile). A circle marks outlier with values between 1.5 and 3 box lengths from the upper or lower edge of the box. Briefly, we found significant main term effects (p = 0.019) “treatment” (LPS and α7nAChR drug) as well as main term “hit” (p = 0.010), i.e., the contribution of *in vivo* LPS exposure, the second hit effect on the IL-1β secretion profile. Results are provided as median {25–75} percentiles. (Right) Identical experimental results from microglia studies are presented for comparison. The main terms “hit” and “treatment” were not significant (p = 0.716 and p = 0.666, respectively), but their interaction was significant (p = 0.026, cf. Figure 1 in (3) where the original results have been published.

From: Cao M, *et al.*.
α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
Front Immunol. 2019 May 9;10:1063.
doi: 10.3389/fimmu.2019.01063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Sheep Interleukin-1 beta

Product Type: Monoclonal Antibody
Clone: 1D4
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1658 Datasheet	C E F* WB SDS	0.25 mg	Log in		Get Quote

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 recognizes ovine interleukin-1 beta, and shows no cross-reactivity with ovine IL-6, IL-8, TNF alpha or MCP-1.

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 demonstrates partial neutralizing activity of ovine IL-1 beta.

Product Details

Target Species

Sheep

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat
Bovine
Horse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Recombinant ovine IL-1 Beta

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P21621
Entrez Gene: IL-1B
GO Terms: GO:0001660 fever generation; GO:0005125 cytokine activity; GO:0005149 interleukin-1 receptor binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008083 growth factor activity; GO:0051781 positive regulation of cell division
Regulatory: For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry ¹			1/10
Immunohistology - Frozen
Western Blotting

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
ELISA: Mouse anti Interleukin-1 beta antibody, clone 1D4 may be used as a capture antibody in a bovine IL-1 beta sandwich ELISA together with Rabbit anti Bovine interleukin-1β antibody (AHP851B) as the detection reagent for evaluation of IL-1β levels in bovine samples together with recombinant Bovine interleukin-1β (PBP008) used as standards.
Alternatively, Mouse anti Interleukin-1 beta antibody, clone 1D4 can be used as a capture reagent together with Rabbit anti Ovine interleukin-1β antibody (AHP423) as a detection reagent for the evaluation of IL-1β levels in ovine, bovine or caprine samples, again utilizing recombinant bovine IL-1&beta (PBP008) as an internal standard.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	F	100 Tests	Log in		Get Quote

Application Based External Images

ELISA

Product Specific References

References for IL-1 Beta antibody

Martoriati, A. & Gérard, N. (2003) Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare.
Reprod Biol Endocrinol. 1: 42-51.
Leite, F. et al. (2005) Incubation of bovine PMNs with conditioned medium from BHV-1 infected peripheral blood mononuclear cells increases their susceptibility to Mannheimia haemolytica leukotoxin.
Vet Immunol Immunopathol. 103 (3-4): 187-93.
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70.
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38.
Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
Clin Vaccine Immunol. 17 (11): 1797-809.
Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72.
Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 42: 49.
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48.
Karisnan K et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS One. 10 (4): e0124390.
Dernfalk, J. et al. (2007) The xMAP technique can be used for detection of the inflammatory cytokines IL-1beta, IL-6 and TNF-alpha in bovine samples.
Vet Immunol Immunopathol. 118 (1-2): 40-9.
Bannerman, D.D. et al. (2004) Characterization of the bovine innate immune response to intramammary infection with Klebsiella pneumoniae.
J Dairy Sci. 87 (8): 2420-32.
Jacobsen, S. et al. (2007) The cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle.
Vet J. 174 (1): 170-5.
Cox, R.A. et al. (2007) Production of pro-inflammatory polypeptides by airway mucous glands and its potential significance.
Pulm Pharmacol Ther. 20 (2): 172-7.
Matthews, K. et al. (2007) The effect of gene gun-delivered pGM-CSF on the immunopathology of the vaccinated skin.
Scand J Immunol. 65 (3): 298-307.
Rainard P et al. (2008) Staphylococcus aureus lipoteichoic acid triggers inflammation in the lactating bovine mammary gland.
Vet Res. 39 (5): 52.
Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
Vet Immunol Immunopathol. 168 (1-2): 97-102.
Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
Reprod Sci. 22 (11): 1409-20.
Sobotta, K. et al. (2016) Coxiella burnetii. Infects Primary Bovine Macrophages and Limits Their Host Cell Response.
Infect Immun. 84 (6): 1722-34.
Cortes, M. et al. (2017) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: A glial in vivo-in vitro multi-hit paradigm in large mammalian brain.
J Neurosci Methods. 276: 23-32.
Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
Res Vet Sci. 110: 34-39.
Cao, M. et al. (2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
Front Immunol. 10: 1063.
Stassi, A.F. et al. (2019) Follicular structures of cows with cystic ovarian disease present altered expression of cytokines.
Zygote. 15: 1-14.

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