IL-1 Beta antibody | 1D4

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Mouse anti Sheep Interleukin-1 beta

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.25 mg loader

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 recognizes ovine interleukin-1 beta, and shows no cross-reactivity with ovine IL-6, IL-8, TNF alpha or MCP-1.

Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 demonstrates partial neutralizing activity of ovine IL-1 beta.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Recombinant ovine IL-1 Beta
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10
Immunohistology - Frozen
Western Blotting
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Mouse anti Interleukin-1 beta antibody, clone 1D4 may be used as a capture antibody in a bovine IL-1 beta sandwich ELISA together with Rabbit anti Bovine interleukin-1β antibody (AHP851B) as the detection reagent for evaluation of IL-1β levels in bovine samples together with recombinant Bovine interleukin-1β (PBP008) used as standards.
Alternatively, Mouse anti Interleukin-1 beta antibody, clone 1D4 can be used as a capture reagent together with Rabbit anti Ovine interleukin-1β antibody (AHP423) as a detection reagent for the evaluation of IL-1β levels in ovine, bovine or caprine samples, again utilizing recombinant bovine IL-1&beta (PBP008) as an internal standard.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images


Product Specific References

References for IL-1 Beta antibody

  1. Martoriati, A. & Gérard, N. (2003) Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare.
    Reprod Biol Endocrinol. 1: 42-51.
  2. Leite, F. et al. (2005) Incubation of bovine PMNs with conditioned medium from BHV-1 infected peripheral blood mononuclear cells increases their susceptibility to Mannheimia haemolytica leukotoxin.
    Vet Immunol Immunopathol. 103 (3-4): 187-93.
  3. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  4. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  5. Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
    Clin Vaccine Immunol. 17 (11): 1797-809.
  6. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  7. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  8. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  9. Karisnan K et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
    PLoS One. 10 (4): e0124390.
  10. Dernfalk, J. et al. (2007) The xMAP technique can be used for detection of the inflammatory cytokines IL-1beta, IL-6 and TNF-alpha in bovine samples.
    Vet Immunol Immunopathol. 118 (1-2): 40-9.
  11. Bannerman, D.D. et al. (2004) Characterization of the bovine innate immune response to intramammary infection with Klebsiella pneumoniae.
    J Dairy Sci. 87 (8): 2420-32.
  12. Jacobsen, S. et al. (2007) The cytokine response of circulating peripheral blood mononuclear cells is changed after intravenous injection of lipopolysaccharide in cattle.
    Vet J. 174 (1): 170-5.
  13. Cox, R.A. et al. (2007) Production of pro-inflammatory polypeptides by airway mucous glands and its potential significance.
    Pulm Pharmacol Ther. 20 (2): 172-7.
  14. Matthews, K. et al. (2007) The effect of gene gun-delivered pGM-CSF on the immunopathology of the vaccinated skin.
    Scand J Immunol. 65 (3): 298-307.
  15. Rainard P et al. (2008) Staphylococcus aureus lipoteichoic acid triggers inflammation in the lactating bovine mammary gland.
    Vet Res. 39 (5): 52.
  16. Doull, L. et al. (2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
    Vet Immunol Immunopathol. 168 (1-2): 97-102.
  17. Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
    Reprod Sci. 22 (11): 1409-20.
  18. Sobotta, K. et al. (2016) Coxiella burnetii. Infects Primary Bovine Macrophages and Limits Their Host Cell Response.
    Infect Immun. 84 (6): 1722-34.
  19. Cortes, M. et al. (2017) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: A glial in vivo-in vitro multi-hit paradigm in large mammalian brain.
    J Neurosci Methods. 276: 23-32.
  20. Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  21. Cao, M. et al. (2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.
    Front Immunol. 10: 1063.
  22. Stassi, A.F. et al. (2019) Follicular structures of cows with cystic ovarian disease present altered expression of cytokines.
    Zygote. 15: 1-14.

Further Reading

  1. Rothel, J.S. et al. (1997) Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry.
    Vet Immunol Immunopathol. 57 (3-4): 267-78.

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