IL-1 Beta

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IL-1 Beta gallery image 1

Published customer image:
Recombinant Bovine Interleukin-1 beta used to create the standard curve for the measurement of IL-β in mik during experimental S. epidermidisand S.simulans infection by ELISA.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-?-) and S. simulans (-?-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.

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IL-1 Beta gallery image 2

Published customer image:
Recombinant Bovine Interleukin-1 beta used to create the standard curve for the measurement of IL-β in mik during experimental S. epidermidisand S.simulans infection by ELISA.
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-?-) and spontaneously eliminated (-¦-) S. epidermidis and S. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.

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  • Recombinant Bovine Interleukin-1 beta
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP008E, FNdatasheet pdfdatasheet pdf20 µg
    PBP008
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Bovine Interleukin-1 beta is a recombinant peptide corresponding to full-length mature Bovine Interleukin-1 beta (IL-1 beta) expressed by a number of cell types including monocytes, macrophages and endothelial cells.

      Interleukin-1 beta, a pro-inflammatory cytokine has a wide range of effects, such as promoting T and B cell proliferation and inducing neutrophil chemotaxis.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified Recombinant Protein - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water.

      Further dilutions should be made in a buffer containing carrier protein.

      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
      40ug/ml after reconstitution
    • Protein Molecular Weight
      17.7kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.5EU/ug
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      Following reconstitution store at -70oC.
      Storage in frost-free freezers is not recommended.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of cell division
      fever generation
      growth factor activity
      extracellular space
      immune response
      interleukin-1 receptor binding
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used as a standard for ELISA applications with the following interleukin-1 beta antibodies: AHP851Z, AHP851B or MCA1658.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-1 Beta Formats

    Formats Applications Sizes available
    IL-1 Beta : Purified E, FN 20 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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