IL-1 Beta Antibody

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IL-1 Beta Antibody gallery image 1

Published customer image:
Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1&beta antibody (MCA1658) as a capture reagent
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-?-) and S. simulans (-?-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis. Vet Res. 2011 Mar 17;42:49.

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IL-1 Beta Antibody gallery image 2

Published customer image:
Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1&beta antibody (MCA1658) as a capture reagent
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-?-) and spontaneously eliminated (-¦-) S. epidermidis and S. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.

From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S. Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis. Vet Res. 2011 Mar 17;42:49.

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  • Rabbit anti Bovine Interleukin-1 beta:Biotin
  • Rabbit anti Bovine Interleukin-1 beta:Preservative Free
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP851BEdatasheet pdfdatasheet pdf0.25 mg
    AHP851B
    AHP851ZEdatasheet pdfdatasheet pdf0.5 mg
    AHP851Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Bovine Interleukin-1 beta antibody recognizes bovine interleukin-1 beta (IL-1 beta).

      IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.

      Rabbit anti Bovine Interleukin-1 beta antibody recognizes both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanno
      Mouseno
      Ratno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1.0ml distilled water
      Care should be taken during reconstitution as the antibody may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
      Recombinant bovine IL-1 beta.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.25mg/ml after reconstitution
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      extracellular space
      immune response
      positive regulation of cell division
      fever generation
      interleukin-1 receptor binding
      growth factor activity
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This anti interleukin-1β:biotin conjugate may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with recombinant IL-1β (PBP008) as the standard and either anti IL-1β:azide free (AHP851Z) or anti IL-1β:purified (MCA1658) as the capture reagents.
    • ELISA
      This antibody may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA system, in combination with AHP851B as the detection antibody. PBP008 may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Beta Antibody Formats

    Formats Applications Sizes available
    IL-1 Beta Antibody : Biotin E 0.25 mg
    IL-1 Beta Antibody : Preservative Free E 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Bovine Interleukin-1 beta:Preservative FreeAHP851Z0.5 mgE
          AHP851Z
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Bovine Interleukin-1 beta:BiotinAHP851B0.25 mgE
          AHP851B

          Recommended Positive Controls

            Histology Controls

              References

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