IgE antibody | 1E7
IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman, F.N. et al. 1997).
IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used in a number of studies involving parasite nematode infections of sheep by Haemonchus contortus (Vervelde, L. et al. 1997 and Kooyman, F.N. et al. 1997). Clone 1E7 also recognizes bovine IgE, and as such it is useful for studies of infection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello, P.A. et al. 2007 and Kooyman, F.N. et al. 2002).
In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Bovine Goat Horse Human
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant ovine IgE1-2 expressed in E.coli
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch
- For research purposes only
Applications of IgE antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
Secondary Antibodies Available
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse anti Bovine IgA||MCA2438GA||E IP||0.1 mg|
|Mouse anti Bovine IgA:HRP||MCA2438P||E||0.25 mg|
|Mouse anti Bovine IgG||MCA2439GA||E IP||0.1 mg|
|Mouse anti Bovine IgG:HRP||MCA2439P||E||0.25 mg|
|Mouse anti Bovine IgG1||MCA2440GA||C E F IP||0.1 mg|
|Mouse anti Bovine IgG1:HRP||MCA2440P||E||0.25 mg|
|Mouse anti Bovine IgG2||MCA2441GA||E F IP||0.1 mg|
|Mouse anti Bovine IgG2:HRP||MCA2441P||E||0.25 mg|
|Mouse anti Bovine IgM||MCA2443GA||E F IP R||0.1 mg|
|Mouse anti Bovine IgG1||MCA627GA||E||0.1 mg|
Product Specific References
References for IgE antibody
Bricarello, P.A. et al. (2007) Field study on nematode resistance in Nelore-breed cattle.
Vervelde, L. et al. (2003) Vaccination-induced protection of lambs against the parasitic nematode Haemonchus contortus correlates with high IgG antibody responses to the LDNF glycan antigen.
Kooyman, F.N. et al. (2002) Serum immunoglobulin E response in calves infected with the lungworm Dictyocaulus viviparus and its correlation with protection.
Parasite Immunol. 24:47-56
Kooyman, F.N. et al. (1997) Production of a monoclonal antibody specifc for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection.