IgE antibody | 1E7
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Mouse anti Sheep IgE
- Product Type
- Monoclonal Antibody
- Clone
- 1E7
- Isotype
- IgG2a
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Mouse anti Sheep IgE, clone 1E7, recognizes ovine Immunoglobulin E (IgE) and does not cross react with ovine IgM, IgA, IgG1 or IgG2. IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman et al. 1997). IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep by Haemonchus contortus (Vervelde et al. 1997 and Kooyman et al. 1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello et al. 2007 and Kooyman et al. 2002). In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad. |
- Target Species
- Sheep
- Species Cross-Reactivity
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Target Species Cross Reactivity Goat Horse Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant ovine IgE1-2 expressed in E.coli
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/2000 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | C E WB | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Bovine IgA | MCA2438GA | E IP | 0.1 mg |
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| Description | Mouse anti Bovine IgA | ||||||
| Mouse anti Bovine IgA:HRP | MCA2438P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgA:HRP | ||||||
| Mouse anti Bovine IgG | MCA2439GA | E IP | 0.1 mg |
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| Description | Mouse anti Bovine IgG | ||||||
| Mouse anti Bovine IgG:HRP | MCA2439P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgG:HRP | ||||||
| Mouse anti Bovine IgG1 | MCA2440GA | C E F IP | 0.1 mg |
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| Description | Mouse anti Bovine IgG1 | ||||||
| Mouse anti Bovine IgG1:HRP | MCA2440P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgG1:HRP | ||||||
| Mouse anti Bovine IgG2 | MCA2441GA | E F IP | 0.1 mg |
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| Description | Mouse anti Bovine IgG2 | ||||||
| Mouse anti Bovine IgG2:HRP | MCA2441P | E | 0.25 mg |
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| Description | Mouse anti Bovine IgG2:HRP | ||||||
| Mouse anti Bovine IgM | MCA2443GA | E F IP R | 0.1 mg |
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| Description | Mouse anti Bovine IgM | ||||||
| Mouse anti Bovine IgG1 | MCA627GA | E | 0.1 mg |
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| Description | Mouse anti Bovine IgG1 | ||||||
References for IgE antibody
-
Kooyman, F.N. et al. (1997) Production of a monoclonal antibody specifc for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection.
Parasitology 114:395-406 -
Kooyman, F.N. et al. (2002) Serum immunoglobulin E response in calves infected with the lungworm Dictyocaulus viviparus and its correlation with protection.
Parasite Immunol. 24:47-56 -
Vervelde, L. et al. (2003) Vaccination-induced protection of lambs against the parasitic nematode Haemonchus contortus correlates with high IgG antibody responses to the LDNF glycan antigen.
Glycobiology 13:795-804 -
Bricarello, P.A. et al. (2007) Field study on nematode resistance in Nelore-breed cattle.
Vet Parasitol.148:272-8
MCA5941GA
148494
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