Secukinumab antibody | AbD37091ia
Human anti Secukinumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD37091ia
- Isotype
- IgG1
- Specificity
- Secukinumab
Human Anti-Secukinumab Antibody, clone AbD37091ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the human monoclonal antibody secukinumab. It does not recognize free human interleukin 17A (IL-17A) nor secukinumab in complex with human IL-17A. The antibody can be used to measure secukinumab or its biosimilars in patient samples. A pair of anti-secukinumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. Either this antibody, or clone AbD37054ia (HCA371), is recommended as the detection antibody, paired with an anti-secukinumab antibody in monovalent Fab format, clone AbD37052 (HCA370), as the capture antibody. Clone AbD37091ia is a fully human recombinant monoclonal antibody with IgG1 isotype and can be used as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 5 nM, and is one of three antibodies with different affinities offered for ADA assays. Secukinumab (Cosentyx) is a human IgG1/kappa antibody generated using transgenic mouse technology. It has been approved for the treatment of plaque psoriasis, ankylosing spondylitis, psoriatic arthritis and non-radiographic axial spondyloarthritis. Secukinumab inhibits IL-17A produced mainly by inflammatory T helper 17 cells (Lubrano and Perrotta 2016). View a summary of all anti- secukinumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Secukinumab
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Secukinumab antibody, clone AbD37091ia is KD = 5 nM as measured by real time, label free molecular interaction analysis on immobilized Secukinumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Cosentyx is a trademark of Novartis. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Secukinumab antibody, clone AbD37091ia is suitable for use as a detection antibody for Secukinumab in a bridging ELISA together with HCA370 as the capture reagent. It can also be used as a surrogate positive control or calibrator in an anti-drug antibody assay.
Protocol: PK bridging ELISA.
Protocol: ADA bridging ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Secukinumab | HCA370 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Secukinumab | ||||||
Human anti Secukinumab | HCA371 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Secukinumab | ||||||
Human anti Secukinumab | HCA373 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Secukinumab | ||||||
Human anti Secukinumab (Drug/Target Complex) | HCA375 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Secukinumab (Drug/Target Complex) | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
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Description | LYNX Rapid HRP Antibody Conjugation Kit |
Further Reading
-
Lubrano, E. & Perrotta, F.M. (2016) Secukinumab for ankylosing spondylitis and psoriatic arthritis.
Ther Clin Risk Manag. 12: 1587-1592.
- Synonyms
- Cosentyx
HCA372
160407 164784If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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