Secukinumab antibody | AbD36949
Human anti Secukinumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD36949
- Isotype
- HuCAL Fab monovalent
- Specificity
- Secukinumab
Human Anti-Secukinumab (Drug/Target Complex) Antibody, clone AbD36949 specifically recognizes the human monoclonal antibody drug secukinumab when in complex with its target, interleukin 17A (IL-17A). The antibody does not recognize free secukinumab or unbound IL-17A. It can be used to measure levels of secukinumab or its biosimilars in patient samples. Clone AbD36949 is a monovalent Fab format antibody that can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free secukinumab that has been captured via immobilized IL-17A. Secukinumab (Cosentyx) is a human IgG1/kappa antibody generated using transgenic mouse technology. It has been approved for the treatment of plaque psoriasis, ankylosing spondylitis, psoriatic arthritis and non-radiographic axial spondyloarthritis. Secukinumab inhibits interleukin 17A (IL-17A) produced mainly by inflammatory T helper 17 cells (Lubrano and Perrotta 2016). View a summary of all anti- secukinumab antibodies |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli.The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Secukinumab/IL-17A complex
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Secukinumab antibody, clone AbD36949 is KD = 7 nM as measured by real time, label free molecular interaction analysis on immobilized hIL-17A/secukinumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Cosentyx is a trademark of Novartis. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Secukinumab antibody, clone AbD36949 is suitable for use as a detection antibody for Secukinumab captured via immobilized hIL-17A.
Protocol: PK antigen capture ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Secukinumab | HCA370 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Secukinumab | ||||||
Human anti Secukinumab | HCA371 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Secukinumab | ||||||
Human anti Secukinumab | HCA372 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Secukinumab | ||||||
Human anti Secukinumab | HCA373 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Secukinumab | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | LYNX Rapid HRP Antibody Conjugation Kit |
Further Reading
-
Lubrano, E. & Perrotta, F.M. (2016) Secukinumab for ankylosing spondylitis and psoriatic arthritis.
Ther Clin Risk Manag. 12: 1587-92.
- Synonyms
- Cosentyx
HCA375
160409If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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