Secukinumab antibody | AbD37052

Human anti Secukinumab

Product Type
Monoclonal Antibody
Clone
AbD37052
Isotype
HuCAL Fab monovalent
Specificity
Secukinumab

Product Code Applications Pack Size List Price Your Price Qty
New HCA370
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Human Anti-Secukinumab Antibody, clone AbD37052 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the human monoclonal antibody secukinumab. It does not recognize free human interleukin 17A (IL-17A) nor secukinumab in complex with human IL-17A. The antibody can be used to measure secukinumab or its biosimilars in patient samples.

Clone AbD37052 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture antibody for secukinumab in a pharmacokinetic (PK) bridging ELISA to measure free drug when paired with either clone AbD37054ia (HCA371) or clone AbD37091ia (HCA372), in IgG1 format, as the detection reagent.

Secukinumab (Cosentyx) is a human IgG1/kappa antibody generated using transgenic mouse technology. It has been approved for the treatment of plaque psoriasis, ankylosing spondylitis, psoriatic arthritis and non-radiographic axial spondyloarthritis. Secukinumab inhibits IL-17A produced mainly by inflammatory T helper 17 cells (Lubrano and Perrotta 2016).

View a summary of all anti- secukinumab antibodies

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Secukinumab
Affinity
The monovalent intrinsic affinity of AbD37052 was measured as KD = 5 nM by real time, label free molecular interaction analysis on immobilized secukinumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Cosentyx is a trademark of Novartis.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD37052 is suitable as a capture antibody for secukinumab in a bridging ELISA together with HCA371 or HCA372 as the detection reagent.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Secukinumab HCA371 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Secukinumab
Human anti Secukinumab HCA372 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Secukinumab
Human anti Secukinumab HCA373 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Secukinumab
Human anti Secukinumab (Drug/Target Complex) HCA375 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Secukinumab (Drug/Target Complex)
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Recombinant Human Interleukin-17A PHP294 E 0.2 mg loader
List Price Your Price
loader
Description Recombinant Human Interleukin-17A

Further Reading

  1. Lubrano, E. & Perrotta, F.M. (2016) Secukinumab for ankylosing spondylitis and psoriatic arthritis.
    Ther Clin Risk Manag. 12: 1587-1592.

Synonyms
Cosentyx
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Secukinumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"