Rituximab antibody | AbD18423_hIgG1
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E F ResetHuman anti Rituximab
- Product Type
- Monoclonal Antibody
- Clone
- AbD18423_hIgG1
- Isotype
- IgG1
- Specificity
- Rituximab
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| Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. Clone AbD18423_hIgG1 is an anti-idiotypic antibody that specifically recognises the rituximab humanised monoclonal antibody. This fully human antibody can be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera |
- Product Form
- Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Rituximab
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=0.13 nM as measured by real time, label-free molecular interaction analysis on immobilized rituximab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP in a sandwich ELISA .
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
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| Description | Rat anti Rituximab:FITC | ||||||
| Rat anti Rituximab:HRP | MCA2260P | E | 0.1 mg |
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| Description | Rat anti Rituximab:HRP | ||||||
| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
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| Human anti Rituximab | HCA186 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| Human anti Rituximab | HCA061 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| Human anti Rituximab | HCA062 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
- Synonyms
- MabThera®
- Rituxan®
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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