Rituximab antibody | AbD18423_hIgG1
Human anti Rituximab
Anti-rituximab antibody is a recombinant anti-idiotypic antibody in fully human immunoglobulin format for bioanalytical method development. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD18423_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA201 | E | 0.1 mg |
|
Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. Clone AbD18423_hIgG1 is an anti-idiotypic antibody that specifically recognises the rituximab humanised monoclonal antibody. This fully human antibody can be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera
Product Details
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Rituximab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Rituximab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP in a sandwich ELISA .
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay. Protocol: ADA bridging ELISA
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Recombinant Human IgG1 Lambda Allotype G1m3 | HCA049 | E | 0.1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Rituximab | HCA061 | E | 0.1 mg |
|
|
| Human anti Rituximab:HRP | HCA061P | E | 0.1 mg |
|
|
| Human anti Rituximab | HCA062 | E | 0.1 mg |
|
|
| Human anti Rituximab:HRP | HCA062P | E | 0.1 mg |
|
|
| Human anti Rituximab | HCA186 | E | 0.1 mg |
|
|
| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
|
|
| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
|
|
| Rat anti Rituximab:HRP | MCA2260P | E | 0.1 mg |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
