Rituximab antibody | AbD18423
Human anti Rituximab
Anti-rituximab antibody is a recombinant anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It is recommended as capture antibody in PK bridging ELISA format with MCA2260P for detection.
- Product Type
- Monoclonal Antibody
- HuCAL Fab monovalent
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This antibody may be used as the capture antibody in a pharmacokinetic bridging ELISA in conjunction with MCA2260 as the detection antibody.
Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
View a summary of all Anti-Rituximab Antibodies.
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Metal chelate affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- The intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- For research purposes only
Applications of Rituximab antibody
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- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Human anti Rituximab||HCA061||0.1 mg||E|
|Human anti Rituximab:HRP||HCA061P||0.1 mg||E|
|Human anti Rituximab||HCA062||0.1 mg||E|
|Human anti Rituximab:HRP||HCA062P||0.1 mg||E|
|Rat anti Rituximab||MCA2260||0.2 mg||E F|
|Rat anti Rituximab:FITC||MCA2260F||0.1 mg||F|
|Rat anti Rituximab:HRP||MCA2260P||0.1 mg||E|
|Mouse anti Penta Histidine Tag:HRP||MCA5995P||125 µl||E WB|
Product Specific References
References for Rituximab antibody
Komori, M. et al. (2016) Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
Ann Clin Transl Neurol. 3 (3): 166-79.