Rituximab antibody | AbD18423

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Human anti Rituximab

Anti-rituximab antibody is a recombinant anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It is recommended as capture antibody in PK bridging ELISA format with MCA2260P for detection.

Product Type
Monoclonal Antibody
Clone
AbD18423
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA186 E 0.1 mg
HCA186G E 1 mg
Human Anti-Rituximab Antibody, clone AbD18423 is a recombinant, anti-idiotypic antibody in monovalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays.

This antibody may be used as the capture antibody in a pharmacokinetic bridging ELISA in conjunction with MCA2260 as the detection antibody.

Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.

View a summary of all Anti-Rituximab Antibodies.

Product Details

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Rituximab
Affinity
The intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Rituximab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Rituximab HCA061 0.1 mg E
Human anti Rituximab:HRP HCA061P 0.1 mg E
Human anti Rituximab HCA062 0.1 mg E
Human anti Rituximab:HRP HCA062P 0.1 mg E
Rat anti Rituximab MCA2260 0.2 mg E F
Rat anti Rituximab:FITC MCA2260F 0.1 mg F
Rat anti Rituximab:HRP MCA2260P 0.1 mg E
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Product Specific References

References for Rituximab antibody

  1. Komori, M. et al. (2016) Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
    Ann Clin Transl Neurol. 3 (3): 166-79.