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Rituximab antibody | AbD18423

Human anti Rituximab

Product Type
Monoclonal Antibody
Clone
AbD18423
Isotype
HuCAL Fab monovalent
Specificity
Rituximab

Product Code Applications Pack Size List Price Your Price Qty
HCA186G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader
HCA186
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Rituximab Antibody, clone AbD18423 is a recombinant, anti-idiotypic antibody in monovalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays.

This antibody may be used as the capture antibody in a pharmacokinetic bridging ELISA in conjunction with MCA2260 as the detection antibody.

Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.

View a summary of all Anti-Rituximab Antibodies.

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
HCA186G: Metal chelate affinity chromatography
HCA186: Metal chelate affinity chromatography.
Source
E.coli
Buffer Solution
HCA186G: Phosphate buffered saline
HCA186: Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Rituximab
Affinity
The intrinsic affinity of this antibody is KD=0.13 nM as measured by real time, label-free molecular interaction analysis on immobilized rituximab.
Approx. Protein Concentrations
HCA186G: Antibody concentration 1.0 mg/ml
HCA186: Antibody concentration 0.5 mg/ml.
Regulatory
HCA186G: For research purposes only
HCA186: For research purposes only.
Guarantee
HCA186G: 12 months from date of despatch
HCA186: 12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Human anti Rituximab HCA061 E 0.1 mg
List Price Your Price
Description Human anti Rituximab
Human anti Rituximab HCA062 E 0.1 mg
List Price Your Price
Description Human anti Rituximab
Rat anti Rituximab MCA2260 E F 0.2 mg loader
List Price Your Price
loader
Description Rat anti Rituximab
Rat anti Rituximab:FITC MCA2260F F 0.1 mg loader
List Price Your Price
loader
Description Rat anti Rituximab:FITC
Rat anti Rituximab:HRP MCA2260P E 0.1 mg loader
List Price Your Price
loader
Description Rat anti Rituximab:HRP
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP

References for Rituximab antibody

  1. Komori, M. et al. (2016) Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
    Ann Clin Transl Neurol. 3 (3): 166-79.

Synonyms
Mabthera
Rituxan
Licensed Use
For in vitro. research purposes and for commercial applications for the provision of in vitro. testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA186G

162190 1709 1803

HCA186

148927A 148927B 157580 164659 164970

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with RITUXIMAB specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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