Rituximab antibody | AbD02842

Human anti Rituximab:HRP

Product Type
Monoclonal Antibody
Clone
AbD02842
Isotype
HuCAL Fab bivalent
Specificity
Rituximab

Product Code Applications Pack Size List Price Your Price Qty
HCA061P
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Human Anti-Rituximab Antibody, clone AbD02842, is a recombinant, anti-idiotypic antibody in bivalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays.

Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.

View a summary of all anti-rituximab antibodies.

Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain, and is conjugated to horseradish peroxidase.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Immunogen
Rituximab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=4.9 nM by real time, label-free molecular interaction analysis on immobilized Rituximab.
Approx. Protein Concentrations
Antibody concentration 0.1mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
MabThera® is a registered trademark of Roche in Europe.
His-tag is a registered trademark of EMD Biosciences.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a sandwich ELISA.
Protocol: PK bridging ELISA to measure free drug.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+
Human anti Rituximab HCA062 E 0.1 mg loader
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Description Human anti Rituximab
Human anti Rituximab HCA186 E 0.1 mg loader
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Description Human anti Rituximab
Rat anti Rituximab MCA2260 E F 0.2 mg loader
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Description Rat anti Rituximab
Rat anti Rituximab:FITC MCA2260F F 0.1 mg loader
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Description Rat anti Rituximab:FITC

References for Rituximab antibody

  1. Warncke, M. et al. (2012) Different adaptations of IgG effector function in human and nonhuman primates and implications for therapeutic antibody treatment.
    J Immunol. 188: 4405-11.

Synonyms
MabThera ®
Rituxan®
RRID
AB_915398
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"