Rituximab antibody | AbD02842
Figure A. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD02842 (HCA061) (red) or Human Fab-dHLX-MH isotype control (HCA052) (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD02842 (HCA061) (red) or Human Fab-dHLX-MH isotype control (HCA052) (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix.
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FN ResetHuman anti Rituximab
- Product Type
- Monoclonal Antibody
- Clone
- AbD02842
- Isotype
- HuCAL Fab bivalent
- Specificity
- Rituximab
Antibody Quality and 
Unrivaled 
| Human Anti-Rituximab Antibody, clone AbD02842, is a recombinant, anti-idiotypic antibody in bivalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells. View a summary of all anti-rituximab antibodies. |
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain - liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Rituximab
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=4.9 nM as measurred by real time, label-free molecular interaction analysis on immobilized Rituximab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
MabThera® is a registered trademark of Roche in Europe.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- In its HRP conjugated format (HCA061P ), this product may be used as a detection reagent in a sandwich ELISA.
Protocol: PK bridging ELISA to measure free drug.
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References for Rituximab antibody
-
Warncke, M. et al. (2012) Different adaptations of IgG effector function in human and nonhuman primates and implications for therapeutic antibody treatment.
J Immunol. 188: 4405-11.
- Synonyms
- MabThera ®
- Rituxan®
- RRID
- AB_915387
- Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Rituximab specificity
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