Rituximab antibody | AbD02842
Human anti Rituximab:HRP
Anti-rituximab antibody is a recombinant anti-idiotypic antibody in bivalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It has been validated as detection antibody in PK bridging ELISA format.
Human anti Rituximab
Anti-rituximab antibody is a recombinant anti-idiotypic antibody in bivalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It has been validated as detection antibody in PK bridging ELISA format.
- Product Type
- Monoclonal Antibody
- Clone
- AbD02842
- Isotype
- HuCAL Fab bivalent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA061P | E | 0.1 mg |
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HCA061 | E | 0.1 mg |
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Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
View a summary of all anti-rituximab antibodies.
Product Details
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain, and is conjugated to horseradish peroxidase.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain - liquid.
- Preparation
- Metal chelate affinity chromatography
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Rituximab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=4.9 nM by real time, label-free molecular interaction analysis on immobilized Rituximab.
- Approx. Protein Concentrations
- Antibody concentration 0.1mg/ml
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
MabThera® is a registered trademark of Roche in Europe.
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
MabThera® is a registered trademark of Roche in Europe.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Rituximab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100 | 1/1000 | |
ELISA | 1/100 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA.
Protocol: PK bridging ELISA to measure free drug. - ELISA
- In its HRP conjugated format (HCA061P ), this product may be used as a detection reagent in a sandwich ELISA.
Protocol: PK bridging ELISA to measure free drug.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml | ||
Human anti Rituximab | HCA062 | E | 0.1 mg |
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Human anti Rituximab | HCA186 | E | 0.1 mg |
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Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
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Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
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Human anti Rituximab:HRP | HCA062P | E | 0.1 mg |
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Human anti Rituximab | HCA186 | E | 0.1 mg |
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Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
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Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
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Product Specific References
References for Rituximab antibody
-
Warncke, M. et al. (2012) Different adaptations of IgG effector function in human and nonhuman primates and implications for therapeutic antibody treatment.
J Immunol. 188: 4405-11.
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