Rituximab antibody | AbD02842
Figure A. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD02842 (HCA061) (red) or Human Fab-dHLX-MH isotype control (HCA052) (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD02842 (HCA061) (red) or Human Fab-dHLX-MH isotype control (HCA052) (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix.
Human anti Rituximab:HRP
Anti-rituximab antibody is a recombinant anti-idiotypic antibody in bivalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It has been validated as detection antibody in PK bridging ELISA format.
Human anti Rituximab
Anti-rituximab antibody is a recombinant anti-idiotypic antibody in bivalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It has been validated as detection antibody in PK bridging ELISA format.
- Product Type
- Monoclonal Antibody
- Clone
- AbD02842
- Isotype
- HuCAL Fab bivalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA061P | E | 0.1 mg |
|
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| HCA061 | E | 0.1 mg |
|
Human Anti-Rituximab Antibody, clone AbD02842, is a recombinant, anti-idiotypic antibody in bivalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays.
Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
View a summary of all anti-rituximab antibodies.
Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.
View a summary of all anti-rituximab antibodies.
Product Details
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain, and is conjugated to horseradish peroxidase.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain - liquid.
- Preparation
- Metal chelate affinity chromatography
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Rituximab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=4.9 nM by real time, label-free molecular interaction analysis on immobilized Rituximab.
- Approx. Protein Concentrations
- Antibody concentration 0.1mg/ml
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up tp 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
MabThera® is a registered trademark of Roche in Europe.
His-tag is a registered trademark of EMD Biosciences. - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
MabThera® is a registered trademark of Roche in Europe.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Rituximab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/1000 | |
| ELISA | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA.
Protocol: PK bridging ELISA to measure free drug. - ELISA
- In its HRP conjugated format (HCA061P ), this product may be used as a detection reagent in a sandwich ELISA.
Protocol: PK bridging ELISA to measure free drug.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
|
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| Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
|
|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| Human anti Rituximab | HCA062 | E | 0.1 mg |
|
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| Human anti Rituximab | HCA186 | E | 0.1 mg |
|
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| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
|
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| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
|
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| Human anti Rituximab:HRP | HCA062P | E | 0.1 mg |
|
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| Human anti Rituximab | HCA186 | E | 0.1 mg |
|
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| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
|
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| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
|
Product Specific References
References for Rituximab antibody
-
Warncke, M. et al. (2012) Different adaptations of IgG effector function in human and nonhuman primates and implications for therapeutic antibody treatment.
J Immunol. 188: 4405-11.
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