CD11b antibody | 198

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Mouse anti Rabbit CD11b:RPE

Product Type: Monoclonal Antibody
Clone: 198
Isotype: IgG1
Specificity: CD11b

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References (14)
Applications
Storage Information
Batch/Lot Specific Datasheets

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MCA802PE Datasheet SDS SDS	F	100 Tests		Log in
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Anti Rabbit CD11b Antibody, clone 198 thumbnail image 1

Anti Rabbit CD11b Antibody, clone 198 thumbnail image 2

Anti Rabbit CD11b Antibody, clone 198 thumbnail image 3

Anti Rabbit CD11b Antibody, clone 198 thumbnail image 4

Anti Rabbit CD11b Antibody, clone 198 gallery image 1 — Flow cytometry analysis of rabbit peripheral blood stained with Anti-Rabbit CD45 PE (MCA808PE) and Mouse IgG1 Negative Control (MCA928A647) (Figure A) or with Anti-Rabbit CD45 PE (MCA808PE) and Anti-Rabbit CD11b A647 (**MCA802A647**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single-cell lymphocytes. Data was acquired on the ZE5 Cell Analyzer.

Anti Rabbit CD11b Antibody, clone 198 gallery image 2 — Flow cytometry analysis of rabbit peripheral blood stained with Anti-Rabbit CD45 Pacific Blue (MCA808PB) and Mouse IgG1 Negative Control (MCA928PE) (Figure A) or with Anti-Rabbit CD45 Pacific Blue (MCA808PB) and Anti-Rabbit CD11b PE (**MCA802PE**) (Figure B). Experiments were performed on red cell lysed blood gated on live, single-cell lymphocytes. Data was acquired on the ZE5 Cell Analyzer.

Anti Rabbit CD11b Antibody, clone 198 gallery image 3 — Flow cytometric staining of rabbit peripheral blood granulocytes with Mouse anti Rabbit CD11b antibody, clone 198 (MCA802GA)

Anti Rabbit CD11b Antibody, clone 198 gallery image 4 — **Published customer image:**
Mouse anti Rabbit CD11b antibody, clone 198 (**MCA802GA**) used to assess CD11b expression levels on rabbit mononuclear cells by flow cytometry.
**Image caption:**
Analysis of *in vivo* BrdU incorporation.
Rabbits were treated as described in Fig. 1. PBMCs were collected at days 11, 15, 17, 20 and 24 post-inoculation, while mononuclear cells were isolated from popliteal lymph node and spleen at the time of death. Cells were labelled with anti-CD11b, IgM, CD5, CD4 and CD8 mAbs as the primary antibodies. Alexa 633-GAM was used as the secondary antibody. *In vivo* BrdU incorporation was revealed by immunofluorescent staining as described in Methods. After staining, cells were analysed by flow cytometry. A. Representative flow cytometry dot plots are shown for each double staining, they illustrate the data obtained at day 17 post-infection for the PBMC of rabbits MR17/1 and IR17/1. The data represent the percentages of BrdU positive cells (y-axis) calculated based on the acquisition of 10,000 cells expressing the indicated cell marker (x-axis). B. The percentage of BrdU positive cells amongst the indicated cellular subset was determined and compared between AlHV-1 infected (left column: bold lines; middle and right columns: hatched bars) and mock infected (left column: dotted lines; middle and right columns: open bars) groups (* P<0.05; **P<0.005, *** P<0.0001). In the left column the following symbols were used: ▴, MR17/1 and IR17/1; ▭, MR17/2 and IR17/2; ●, MR20 and IR20; ○, MR24 and IR24.

From: Dewals B, Boudry C, Farnir F, Drion P-V, Vanderplasschen A (2008)
Malignant Catarrhal Fever Induced by Alcelaphine herpesvirus 1 Is Associated with Proliferation of CD8⁺ T Cells Supporting a Latent Infection.
**PLoS ONE 3(2): e1627**.
doi: 10.1371/journal.pone.0001627.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Rabbit CD11b antibody, clone 198 recognizes the rabbit CD11b cell surface glycoprotein, also known as the integrin alpha M chain and MAC-1. Mouse anti Rabbit CD11b antibody, clone 198 immunoprecipitates two proteins of molecular weight 165 kD and 95 kD from granulocytes. It recognizes monocytes, macrophages and neutrophils by flow cytometry and is thought to be against the homologue of human CD11b. In immunohistochemistry good staining of macrophages is observed.

Target Species: Rabbit
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Immunogen: Rabbit adherent blood leucocytes.
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0.Ag14 mouse myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

RPE 488nm laser 496 578

RPE 561nm laser 546 578
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

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Source Reference

Smet, E.G. et al. (1986) Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit.
Immunology. 59 (3): 419-25.

References for CD11b antibody

Wilkinson, J.M. et al. (1993) Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit.
J Pathol. 170 (3): 315-20.
Brickson, S. et al. (2003) M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
J Appl Physiol. 95: 969-76.
Hoefer, I.E. et al. (2005) Aspirin, but not clopidogrel, reduces collateral conductance in a rabbit model of femoral artery occlusion.
J Am Coll Cardiol. 46 (6): 994-1001.
Fábián, K. et al. (2005) Detection of bovine herpesvirus 4 in CD11b+ leukocytes of experimentally infected rabbits.
Acta Vet Hung. 53 (2): 265-73.
Georgiadis, P. et al. (2008) Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
Stroke. 39: 3378-88.
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLos ONE 3: e1627.
Gillet, L. et al. (2009) Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity.
Vet Res. 40: 51.
Xu, Y. et al. (2010) Adenovirus-mediated overexpression of glutathione-s-transferase mitigates transplant arteriosclerosis in rabbit carotid allografts.
Transplantation. 89: 409-16.

View The Latest Product References

Vinukonda, G. et al. (2010) Neuroprotection in a rabbit model of intraventricular haemorrhage by cyclooxygenase-2, prostanoid receptor-1 or tumour necrosis factor-alpha inhibition.
Brain. 133 (Pt 8): 2264-80.
Vinukonda, G. et al. (2016) Hyaluronidase and Hyaluronan Oligosaccharides Promote Neurological Recovery after Intraventricular Hemorrhage.
J Neurosci. 36 (3): 872-89.
Lin, W. et al. (2020) Rapid identification of anti-idiotypic mAbs with high affinity and diverse epitopes by rabbit single B-cell sorting-culture and cloning technology.
PLoS One. 15 (12): e0244158.
Noreng, S. et al. (2022) Structure of the core human NADPH oxidase NOX2.
Nat Commun. 13 (1): 6079.
Türk, Ü.B. et al. (2024) Alterations in the spinal cord, trigeminal nerve ganglion, and infraorbital nerve through inducing compression of the dorsal horn region at the upper cervical cord in trigeminal neuralgia.
Brain Res. 1832: 148842.