CD144 antibody | 123
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Rat anti Pig CD144
- Product Type
- Monoclonal Antibody
- Clone
- 123
- Isotype
- IgG2a
- Specificity
- CD144
Antibody Quality and Performance Guarantee
| Rat anti Pig CD144 antibody, clone 123 recognizes a ~120-130 kDa a single pass type I membrane protein considered to be the porcine homologue of human CD144, also known as VE-Cadherin or Cadherin-5. VE-cadherin is expressed by endothelial cells and is important in cell-cell adhesion, tight junction integrity and vascular permeabiltiy (Tinsley et al. 1999). VE-Cadherin is highly susceptible to proteolytic degradation. Western blotting and immunofluorescence using Rat anti Porcine CD144 antibody, clone 123 demonstrates rearrangement and upregulation of VE-cadherin in porcine endothelial cells cultured as part of tissue engineered blood vessels under shear stress conditions rather than static conditions (Lee et al. 2012). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine VE-cadherin/human IgG Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for CD144 antibody
-
Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
Transplantation. 68 (6): 861-7. -
Hoffmann, J. et al. (2008) Immobilized DNA aptamers used as potent attractors for porcine endothelial precursor cells.
J Biomed Mater Res A. 84 (3): 614-21. -
Allen, J. et al. (2008) Characterization of porcine circulating progenitor cells: toward a functional endothelium.
Tissue Eng Part A. 14 (1): 183-94. -
Zhong, A. et al. (2018) The Roles of Matrix Stiffness and β-Catenin Signaling in Endothelial-to-Mesenchymal Transition of Aortic Valve Endothelial Cells.
Cardiovasc Eng Technol. 9 (2): 158-67. -
Bernardini, C. et al. (2023) Isolation of Vascular Wall Mesenchymal Stem Cells from the Thoracic Aorta of Adult Göttingen Minipigs: A New Protocol for the Simultaneous Endothelial Cell Collection.
Animals (Basel). 13 (16)Aug 12 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- VE-CADHERIN
- UniProt
- O02840
- Entrez Gene
- CDH5
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0007156 homophilic cell adhesion
- GO:0030054 cell junction
MCA1748GA
1607
164614
167470
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