CD144 antibody | 123
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Rat anti Pig CD144 antibody, clone 123 recognizes a ~120-130 kDa a single pass type I membrane protein considered to be the porcine homologue of human CD144, also known as VE-Cadherin or Cadherin-5.
VE-cadherin is expressed by endothelial cells and is important in cell-cell adhesion, tight junction integrity and vascular permeabiltiy (Tinsley et al. 1999). VE-Cadherin is highly susceptible to proteolytic degradation.
Western blotting and immunofluorescence studies with Rat anti Porcine CD144 antibody, clone 123 demonstrate rearrangement and upregulation of VE-cadherin in porcine endothelial cells cultured as part of tissue engineered blood vessels under shear stress conditions rather than static conditions (Lee et al. 2012).
VE-cadherin is expressed by endothelial cells and is important in cell-cell adhesion, tight junction integrity and vascular permeabiltiy (Tinsley et al. 1999). VE-Cadherin is highly susceptible to proteolytic degradation.
Western blotting and immunofluorescence studies with Rat anti Porcine CD144 antibody, clone 123 demonstrate rearrangement and upregulation of VE-cadherin in porcine endothelial cells cultured as part of tissue engineered blood vessels under shear stress conditions rather than static conditions (Lee et al. 2012).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine VE-cadherin/human IgG Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O02840
- Entrez Gene
- CDH5
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0007156 homophilic cell adhesion
- GO:0030054 cell junction
- Regulatory
- For research purposes only
Applications of CD144 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Product Specific References
References for CD144 antibody
-
Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
Transplantation. 68 (6): 861-7. -
Allen, J. et al. (2008) Characterization of porcine circulating progenitor cells: toward a functional endothelium.
Tissue Eng Part A. 14 (1): 183-94. -
Hoffmann, J. et al. (2008) Immobilized DNA aptamers used as potent attractors for porcine endothelial precursor cells.
J Biomed Mater Res A. 84 (3): 614-21. -
Tsai, S.H. et al. (2007) Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering.
Biochem Biophys Res Commun. 357 (4): 984-90. -
Zhong, A. et al. (2018) The Roles of Matrix Stiffness and β-Catenin Signaling in Endothelial-to-Mesenchymal Transition of Aortic Valve Endothelial Cells.
Cardiovasc Eng Technol. 9 (2): 158-67.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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