Omalizumab antibody | AbD20669

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Human anti Omalizumab

Anti-omalizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development for omalizumab and biosimilars. It is suitable as a capture antibody in PK bridging ELISA format with HCA235 for an assay to detect free drug.

Product Type
Monoclonal Antibody
Clone
AbD20669
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA236 E 0.1 mg
Omalizumab (brand name Xolair®) is a recombinant DNA-derived humanized IgG1 kappa monoclonal antibody used in the treatment of patients with moderate or severe asthma who have demonstrated a positive allergy skin test and whose symptoms are not controlled by inhaled corticosteroids.

Allergic asthma is mediated by IgE released by B cells in response to allergen. Circulating IgE binds to the high-affinity IgE Fc receptor (FcεRI) expressed on basophils and mast cells, triggering the release of histamine, leukotrienes and other mediators associated with the pathophysiology of asthma. Omalizumab is directed against the Fc region of human immunoglobulin E (IgE). By binding to circulating IgE at the site of FcεRI binding, this therapeutic antibody prevents the interaction of IgE with its receptor thus limiting mediator release. Treatment with omalizumab has also been demonstrated to reduce the expression of FcεRI on mast cells and basophils, providing additional clinical benefit.

Human anti omalizumab, clone AbD20669 is a paratope specific anti-idiotypic (Type 1) antibody that binds specifically to omalizumab but not to free IgE or the drug/immunoglobulin complex. This antibody can be used to measure omalizumab levels in serum from patients. Clone AbD20669 is particularly suited for use as a capture reagent in Pharmacokinetic (PK) assay development.

Other Type 1 (HCA235) and Type 3 (HCA237, HCA238) antibodies to omalizumab are also available from Bio-Rad in IgG1 and Fab formats. This range of anti omalizumab antibodies forms part of a wider and expanding portfolio of anti-idiotypic antibodies that have been developed for the detection of biotherapeutics.

Product Details

Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Omalizumab.
Affinity
The monovalent intrinsic affinity of HCA236 was measured as Kd = 1.1 nM by real time, label free molecular interaction analysis on immobilized omalizumab
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

Xolair® is a registered trademark of Novartis.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Omalizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a capture reagent in PK assay development together with HCA235P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049B 125 ml E IY
Recombinant Human IgE Lambda HCA171 0.1 mg E
Recombinant Human IgE Kappa HCA190 0.1 mg E
Human anti Omalizumab HCA236 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK001P 1 Conjugation For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK003P 1 Conjugation For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK004P 5 Conjugations For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK005P 1 Conjugation For 20mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK006P 3 Conjugations For 40µg Antibody CJ
Rat anti DYKDDDDK Tag:HRP MCA4764P 0.1 mg E P WB