Omalizumab antibody | AbD20669

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Human anti Omalizumab

Anti-omalizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development for omalizumab and biosimilars. It is suitable as a capture antibody in PK bridging ELISA format with HCA235 for an assay to detect free drug.

Product Type
Monoclonal Antibody
Clone
AbD20669
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
HCA236
Datasheet
E
SDS
0.1 mg loader

Product Details

Product Form
A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Affinity
The monovalent intrinsic affinity of HCA236 was measured as Kd = 1.1 nM by real time, label free molecular interaction analysis on immobilized omalizumab
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

Xolair® is a registered trademark of Novartis.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Omalizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a capture reagent in PK assay development together with HCA235P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049B E IY 125 ml
Recombinant Human IgE Lambda HCA171 E 0.1 mg loader
Recombinant Human IgE Kappa HCA190 E 0.1 mg loader
Human anti Omalizumab HCA236 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK003P CJ 1 Conjugation For 4mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK004P CJ 5 Conjugations For 4mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK005P CJ 1 Conjugation For 20mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK006P CJ 3 Conjugations For 40µg Antibody
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg loader

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