TNF Alpha antibody | MP6-XT3
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003).
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant murine TNF-alpha expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P06804 Related reagents
- Entrez Gene
- Tnf Related reagents
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0001891 phagocytic cup
- GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- GO:0006006 glucose metabolic process
- GO:0006927 transformed cell apoptosis
- GO:0006959 humoral immune response
- GO:0007254 JNK cascade
- GO:0009887 organ morphogenesis
- GO:0050830 defense response to Gram-positive bacterium
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0009897 external side of plasma membrane
- GO:0030198 extracellular matrix organization
- GO:0030316 osteoclast differentiation
- GO:0031622 positive regulation of fever generation
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045121 membrane raft
- GO:0045670 regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0045994 positive regulation of translational initiation by iron
- GO:0046325 negative regulation of glucose import
- GO:0046330 positive regulation of JNK cascade
- GO:0051023 regulation of immunoglobulin secretion
- GO:0051798 positive regulation of hair follicle development
- GO:0055037 recycling endosome
- GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
- GO:0060693 regulation of branching involved in salivary gland morphogenesis
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | 5ug/ml | |
| Flow Cytometry 1 | |||
| Functional Assays | |||
| Western Blotting | |||
| ELISA | 5ug/ml | ||
| Flow Cytometry 1 | |||
| Western Blotting |
- 1Membrane permeabilisation is required prior to staining in Flow Cytometry. We recommend the use of Leucoperm™ (BUF09A) for this purpose.
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG1 Negative Control:Low Endotoxin | MCA1211EL | 0.5 mg | C E F | ||
| Rat IgG1 Negative Control | MCA1211 | 1 ml | C E F |
Product Specific References
References for TNF Alpha antibody
-
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24. -
Bauman, S.K. et al. (2003) Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.
Infect Immun. 71 (1): 68-74. -
Hostager BS, & Bishop GA. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b.
J Immunol. 168: 3318-22. -
Huffnagle, G.B. et al. (1996) Afferent phase production of TNF-alpha is required for the development of protective T cell immunity to Cryptococcus neoformans.
J Immunol. 157: 4529-36. -
Reljic, R. et al. (2004) Mouse IgA inhibits cell growth by stimulating tumor necrosis factor-alpha production and apoptosis of macrophage cell lines.
Int Immunol. 16 (4): 607-14.
