Rat anti Mouse TNF alpha antibody, clone MP6-XT3
recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.
Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Recombinant murine TNF-alpha expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
negative regulation of transcription from RNA polymerase II promoter
positive regulation of chronic inflammatory response to antigenic stimulus
positive regulation of humoral immune response mediated by circulating immunoglobulin
integral to plasma membrane
tumor necrosis factor receptor binding
stored secretory granule
glucose metabolic process
transformed cell apoptosis
humoral immune response
defense response to Gram-positive bacterium
induction of apoptosis via death domain receptors
external side of plasma membrane
extracellular matrix organization
positive regulation of fever generation
positive regulation of interferon-gamma production
positive regulation of interleukin-6 production
tumor necrosis factor-mediated signaling pathway
negative regulation of growth of symbiont in host
positive regulation of I-kappaB kinase/NF-kappaB cascade
regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of translational initiation by iron
negative regulation of glucose import
positive regulation of JNK cascade
regulation of immunoglobulin secretion
positive regulation of hair follicle development
epithelial cell proliferation involved in salivary gland morphogenesis
regulation of branching involved in salivary gland morphogenesis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of TNF Alpha antibody
|Flow Cytometry 1
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
References for TNF Alpha antibody
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Bauman, S.K. et al. (2003) Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.
Infect Immun. 71 (1): 68-74.
Hostager BS, & Bishop GA. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b.
J Immunol. 168: 3318-22.
Huffnagle, G.B. et al. (1996) Afferent phase production of TNF-alpha is required for the development of protective T cell immunity to Cryptococcus neoformans.
J Immunol. 157: 4529-36.
Reljic, R. et al. (2004) Mouse IgA inhibits cell growth by stimulating tumor necrosis factor-alpha production and apoptosis of macrophage cell lines.
Int Immunol. 16 (4): 607-14.
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