IgG2a antibody | AbD24124

Human anti Mouse IgG2a:HRP

Product Type
Monoclonal Antibody
Clone
AbD24124
Isotype
HuCAL Fab bivalent
Specificity
IgG2a

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HCA310P
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Human anti Mouse IgG2a monoclonal antibody, clone AbD24124, recognizes the IgG2a isotype of the primary antibody, with no species cross reactivity against mouse IgG1 and mouse IgG2b primary antibodies. This antibody allows for more than one unlabelled mouse monoclonal antibody to be used at any one time permitting multiplexing.

Target Species
Mouse
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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