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IgG2a antibody | AbD24124

Human anti Mouse IgG2a:DyLight®550

Product Type
Monoclonal Antibody
HuCAL Fab bivalent

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
EN F IF WB 0.1 mg loader
List Price Your Price

Human anti Mouse IgG2a monoclonal antibody, clone AbD24124, recognizes the IgG2a isotype of the primary antibody, with no species cross reactivity against mouse IgG1 and mouse IgG2b primary antibodies. This antibody allows for more than one unlabelled mouse monoclonal antibody to be used at any one time permitting multiplexing.

Human anti Mouse IgG2a antibody, clone AbD24124, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology.
Rare Cell/CTC Enumeration

Target Species
Product Form
A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied conjugated to DyLight 550 - liquid.
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Dylight®550 562 576
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a trademark of EMD Biosciences.
DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
CelSelect Slides is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/500 1/10,000
Immunofluorescence 1/100 1/1000
Rare Cell / CTC Enumeration 1/100 1/500
Western Blotting 1/1000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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