ER-TR7 antibody | ER-TR7
Rat anti mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used for the identification of fibroreticular networks in mouse lymph node cryosections by immunofluorescence.
Image caption:
Immunohistochemical localization of MGL2+ DDCs, LCs and LN conduits.
(B) Distribution of MGL2 (red), PNAd (HEV), Langerin (LC) or ER-TR7 antigen (FRN) (blue in each panel) in the sensitization process. Areas indicated by the squares are magnified in the lower panels. FITC is shown in green. CR associated with HEV in a naive LN is adjacent to MGL2+ DDC only at its follicular side but not at the cortical side. Arrowheads indicate association of MGL2+ DDCs with ER-TR7+ FRN around HEV-associated CR. Scale bar, 100 μm.
From: Kumamoto Y, Denda-Nagai K, Aida S, Higashi N, Irimura T (2009)
MGL2+ Dermal Dendritic Cells Are Sufficient to Initiate Contact Hypersensitivity In Vivo.
PLoS ONE 4(5): e5619.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on formalin fixed, paraffin embedded sections.
Image caption:
ER‐TR7 outlines tissue compartments of the digit. (A) H&E section of PN11 mouse digit tip shows compartments including nail bed (nb), ventral epithelium (ve), eccrine glands (eg), and a P3 rudiment composed of both cortical bone (b) and a proximal cartilaginous (c) growth plate. P3 encloses bone marrow (bm) and ends at the P3−P2 synovial joint (jt). P3 is connected to the proximal musculature through a tendon (tn) and is surrounded by loose dorsal and ventral CT (dct and vct). (B) Adjacent section from (A) stained against ER‐TR7. (C) Representative area captured at 400× from the dct in (B) (white asterisk). The boundary landmarks of the CT (labeled nb and b) are outlined with white dotted lines. ER‐TR7+ FRCs are marked (white + signs on nuclei) and these were discriminated (C, inset) at 1000× magnification by ER‐TR7 expression in membrane extensions (white arrows) or cytosol (white asterisk) of individual cells. Scale bars (A), (B) 50 μm and (C) 25 μm. Serial sections were also co‐immunostained for (D) ER‐TR7, FVIII, and SMA (white × marks negative cells) or (E) ER‐TR7 and osteocalcin OC; scale bars (D)−(E) 10 μm.
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on formalin fixed, paraffin embedded sections.
Image caption:
ER‐TR7 is upregulated during neonatal and adult digit tip blastema formation. ER‐TR7 (red) expression following digit tip amputation of PN3 neonates and 8W adult mice was detected by indirect immunofluorescence counterstained with DAPI (gray), captured at 100× magnification (scale bar 50 μmn . N= 4 animals per group). Images of select timepoints prior to and following amputation are shown for both (A)−(C) neonates and (E)−(G) adult mouse digit tips. The amputation level for both (A) neonate and (E) adult UA0 digits is marked by a yellow line. Scale bars (A)−(D) and (F)−(I) 50 μm.
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on formalin fixed, paraffin embedded sections.
Image caption:
Expansion of ER‐TR7+ FRCs is specific to the blastema stage. In representative (A) UA8 and (C) DPA8 samples, these areas are flanked by the nail bed (nb) and the P3 bone (b) and outlined with a dotted white line (scale bar 50 μm). Proliferating (Ki67+) cells were grouped by ER‐TR7+ or ER‐TR7− expression. (A), (C), insets: ER‐TR7+/Ki67+ cells were discriminated at 1000× magnification and are labeled with white + signs. ER‐TR7−/Ki67+ cells are marked with a − sign (scale bar 10 μm).
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on formalin fixed, paraffin embedded sections.
Image caption:
ER‐TR7 shares an expression trend with COL3. Representative
(A)−(C) UA8 samples from groups co‐stained for ER‐TR7 and COL3. Shown are individual (A) COL3 and (B) ER‐TR7 channels in grayscale with (C) merged with DAPI color images (scale bar 100 μm). (C) insert: Z‐stack captured from region of interest at 600× magnification (white‐outlined rectangles on corresponding low magnification image) in UA8 sample was rendered in 3D format to depict the subcellular distribution of the markers (grid 10 μm). The trend between the two antigens in vivo was measured by PCC‐based analysis with mean ± SE values (n = 4) shown on corresponding (C) merged channel images, where +1.0 is equivalent to a perfect trend and −1.0 equivalent to an absolutely opposite trend.
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on formalin fixed, paraffin embedded sections.
Image caption:
ER‐TR7 shares an expression trend with COL3. Representative
(D)−(F) UA8 samples from groups co‐stained for ER‐TR7 and COL3. Shown are individual (D) COL3 and (E) ER‐TR7 channels in grayscale with (F) merged with DAPI color images (scale bar 100 μm). (F) insert: Z‐stack captured from region of interest at 600× magnification (white‐outlined rectangles on corresponding low magnification image) in UA8 sample was rendered in 3D format to depict the subcellular distribution of the markers (grid 10 μm). The trend between the two antigens in vivo was measured by PCC‐based analysis with mean ± SE values (n = 4) shown on corresponding (F) merged channel image, where +1.0 is equivalent to a perfect trend and −1.0 equivalent to an absolutely opposite trend.
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on isolated P3 cells.
Image caption:
ER‐TR7+ filament induction in P3 cells.
(A) Freshly isolated blastema cells retain the intercellular ER‐TR7 network in vitro (630×; scale bar 10 μm).
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on isolated P3 cells.
Image caption:
ER‐TR7+ filament induction in P3 cells. (B)−(G) Cells isolated from digit tips harvested from eGFP transgenic mice were split into (B)−(D) untreated control and (E)−(G) TNFα + anti‐LTβR induced lines (representative fields at 400×; scale bar 50 μm).
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used to demonstrate ER-TR7 expressing cells in murine digits by immunofluorescence on isolated P3 cells.
Image caption:
Co‐IHC localization studies on P3 cells co‐treated with TNFα and anti‐LTβR. Co‐localization analysis based on the PCC approach over eight high resolution photomicrographs at 400× magnification from each culture was performed (Fig. S3). Trends in localization and intensity level for these two antigens in both uninduced (Fig. S3A−C) and treated (Fig. S3D−F) P3 cells were almost identical gauged by our qualitative observations and strong pixel correlation measurements with overall mean r p values above 0.8.
From: Marrero L, Simkin J, Sammarco M, Muneoka K.
Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 2017 May 3;4(2):69-84.
doi: 10.1002/reg2.75.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Isolated C3H thymic stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of ER-TR7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/50 | 1/100 |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Spleen
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
Source Reference
-
VanVliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
Eur J Immunol. 14 (6): 524-9.
References for ER-TR7 antibody
-
Van Vliet, E. et al. (1986) Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
J Histochem Cytochem. 34 (7): 883-90. -
Kalled, S.L. et al. (1998) Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function.
J Immunol. 160: 2158-65. -
Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
J Histochem Cytochem. 58 (2): 183-93. -
Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
Blood. 116: 3456-64. -
Kumamoto, Y. et al. (2009) MGL2 Dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo.
PLoS One. 4: e5619. -
Fujii, N. et al. (2006) Targeting of interstitial cells using a simple gene-transfer strategy.
Nephrol Dial Transplant. 21: 2745-53. -
Katakai, T. et al. (2003) Th1-biased tertiary lymphoid tissue supported by CXC chemokine ligand 13-producing stromal network in chronic lesions of autoimmune gastritis.
J Immunol. 171: 4359-68. -
Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
Proc Natl Acad Sci U S A. 104:15430-5. -
Bennett, K.M. et al. (2016) Induction of Colonic M Cells during Intestinal Inflammation.
Am J Pathol. 186 (5): 1166-79. -
Burrell, B.E. et al. (2015) Lymph Node Stromal Fiber ER-TR7 Modulates CD4+ T Cell Lymph Node Trafficking and Transplant Tolerance.
Transplantation. 99 (6): 1119-25. -
Umemoto, E. et al. (2012) Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling.
J Immunol. 189 (1): 191-9. -
Watanabe, R. et al. (2016) Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus.
Neuropathology. Apr 28. [Epub ahead of print] -
Marrero, L. et al. (2017) Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 4 (2): 69-84. -
Dawson, L.A. et al. (2020) Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration.
Wound Repair Regen. Aug 19 [Epub ahead of print].
Further Reading
-
Lokmic, Z. et al. (2008) The extracellular matrix of the spleen as a potential organizer of immune cell compartments.
Semin Immunol. 20: 4-13.
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