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ER-TR7 antibody | ER-TR7

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Anti Mouse ER-TR7 Antibody, clone ER-TR7 gallery image 1
Published customer image:
Rat anti mouse ER-TR7 antibody, clone ER-TR7 (MCA2402) used for the identification of fibroreticular networks in mouse lymph node cryosections by immunofluorescence.
Image caption:
Immunohistochemical localization of MGL2+ DDCs, LCs and LN conduits.
(B) Distribution of MGL2 (red), PNAd (HEV), Langerin (LC) or ER-TR7 antigen (FRN) (blue in each panel) in the sensitization process. Areas indicated by the squares are magnified in the lower panels. FITC is shown in green. CR associated with HEV in a naive LN is adjacent to MGL2+ DDC only at its follicular side but not at the cortical side. Arrowheads indicate association of MGL2+ DDCs with ER-TR7+ FRN around HEV-associated CR. Scale bar, 100 μm.

From: Kumamoto Y, Denda-Nagai K, Aida S, Higashi N, Irimura T (2009)
MGL2+ Dermal Dendritic Cells Are Sufficient to Initiate Contact Hypersensitivity In Vivo.
PLoS ONE 4(5): e5619.

Rat anti Mouse ER-TR7

Product Type
Monoclonal Antibody
Clone
ER-TR7
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2402
Datasheet
C F* IF
MSDS
0.25 mg loader

Rat anti Mouse ER-TR7 antibody, clone ER-TR7 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Isolated C3H thymic stromal cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of ER-TR7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Spleen
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

Source Reference

  1. VanVliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
    Eur J Immunol. 14 (6): 524-9.

References for ER-TR7 antibody

  1. Van Vliet, E. et al. (1986) Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
    J Histochem Cytochem. 34 (7): 883-90.
  2. Kalled, S.L. et al. (1998) Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function.
    J Immunol. 160: 2158-65.
  3. Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
    J Histochem Cytochem. 58 (2): 183-93.
  4. Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
    Blood. 116: 3456-64.
  5. Kumamoto, Y. et al. (2009) MGL2 Dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo.
    PLoS One. 4: e5619.
  6. Fujii, N. et al. (2006) Targeting of interstitial cells using a simple gene-transfer strategy.
    Nephrol Dial Transplant. 21: 2745-53.
  7. Katakai, T. et al. (2003) Th1-biased tertiary lymphoid tissue supported by CXC chemokine ligand 13-producing stromal network in chronic lesions of autoimmune gastritis.
    J Immunol. 171: 4359-68.
  8. Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
    Proc Natl Acad Sci U S A. 104:15430-5.
  9. Bennett, K.M. et al. (2016) Induction of Colonic M Cells during Intestinal Inflammation.
    Am J Pathol. 186 (5): 1166-79.
  10. Burrell, B.E. et al. (2015) Lymph Node Stromal Fiber ER-TR7 Modulates CD4+ T Cell Lymph Node Trafficking and Transplant Tolerance.
    Transplantation. 99 (6): 1119-25.
  11. Umemoto, E. et al. (2012) Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling.
    J Immunol. 189 (1): 191-9.
  12. Watanabe, R. et al. (2016) Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus.
    Neuropathology. Apr 28. [Epub ahead of print]

Further Reading

  1. Lokmic, Z. et al. (2008) The extracellular matrix of the spleen as a potential organizer of immune cell compartments.
    Semin Immunol. 20: 4-13.
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