ER-TR7 antibody | ER-TR7
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Rat anti Mouse ER-TR7
- Product Type
- Monoclonal Antibody
|Rat anti Mouse ER-TR7 antibody, clone ER-TR7 recognizes ER-TR7, an antigen located in the cytoplasm of reticular fibroblasts, a component of the extracellular matrix of lymphoid and non-lymphoid organs.
Rat anti Mouse ER-TR7 antibody, clone ER-TR7 recognizes collagen type VI (Schiavinato et al. 2021) and has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Isolated C3H thymic stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1|
|Immunohistology - Frozen||1/50||1/100|
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Histology Positive Control Tissue
- Mouse spleen
VanVliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
Eur J Immunol. 14 (6): 524-9.
References for ER-TR7 antibody
Van Vliet, E. et al. (1986) Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
J Histochem Cytochem. 34 (7): 883-90.
Kalled, S.L. et al. (1998) Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function.
J Immunol. 160: 2158-65.
Katakai, T. et al. (2003) Th1-biased tertiary lymphoid tissue supported by CXC chemokine ligand 13-producing stromal network in chronic lesions of autoimmune gastritis.
J Immunol. 171: 4359-68.
Fujii, N. et al. (2006) Targeting of interstitial cells using a simple gene-transfer strategy.
Nephrol Dial Transplant. 21: 2745-53.
Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
Proc Natl Acad Sci U S A. 104:15430-5.
Kumamoto, Y. et al. (2009) MGL2 Dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo.
PLoS One. 4: e5619.
Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
Blood. 116: 3456-64.
Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
J Histochem Cytochem. 58 (2): 183-93.
View The Latest Product References
Umemoto, E. et al. (2012) Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling.
J Immunol. 189 (1): 191-9.
Burrell, B.E. et al. (2015) Lymph Node Stromal Fiber ER-TR7 Modulates CD4+ T Cell Lymph Node Trafficking and Transplant Tolerance.
Transplantation. 99 (6): 1119-25.
Bennett, K.M. et al. (2016) Induction of Colonic M Cells during Intestinal Inflammation.
Am J Pathol. 186 (5): 1166-79.
Watanabe, R. et al. (2016) Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus.
Neuropathology. 36 (6): 513-26.
Marrero, L. et al. (2017) Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 4 (2): 69-84.
Dawson, L.A. et al. (2020) Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration.
Wound Repair Regen. Aug 19 [Epub ahead of print].
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