ER-TR7 antibody | ER-TR7









Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Isolated C3H thymic stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of ER-TR7 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | |||
Immunofluorescence | |||
Immunohistology - Frozen | 1/50 | 1/100 |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Spleen
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Product Specific References
Source Reference
-
VanVliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
Eur J Immunol. 14 (6): 524-9.
References for ER-TR7 antibody
-
Van Vliet, E. et al. (1986) Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
J Histochem Cytochem. 34 (7): 883-90. -
Kalled, S.L. et al. (1998) Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function.
J Immunol. 160: 2158-65. -
Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
J Histochem Cytochem. 58 (2): 183-93. -
Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
Blood. 116: 3456-64. -
Kumamoto, Y. et al. (2009) MGL2 Dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo.
PLoS One. 4: e5619. -
Fujii, N. et al. (2006) Targeting of interstitial cells using a simple gene-transfer strategy.
Nephrol Dial Transplant. 21: 2745-53. -
Katakai, T. et al. (2003) Th1-biased tertiary lymphoid tissue supported by CXC chemokine ligand 13-producing stromal network in chronic lesions of autoimmune gastritis.
J Immunol. 171: 4359-68. -
Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
Proc Natl Acad Sci U S A. 104:15430-5. -
Bennett, K.M. et al. (2016) Induction of Colonic M Cells during Intestinal Inflammation.
Am J Pathol. 186 (5): 1166-79. -
Burrell, B.E. et al. (2015) Lymph Node Stromal Fiber ER-TR7 Modulates CD4+ T Cell Lymph Node Trafficking and Transplant Tolerance.
Transplantation. 99 (6): 1119-25. -
Umemoto, E. et al. (2012) Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling.
J Immunol. 189 (1): 191-9. -
Watanabe, R. et al. (2016) Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus.
Neuropathology. Apr 28. [Epub ahead of print] -
Marrero, L. et al. (2017) Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
Regeneration (Oxf). 4 (2): 69-84. -
Dawson, L.A. et al. (2020) Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration.
Wound Repair Regen. Aug 19 [Epub ahead of print].
Further Reading
-
Lokmic, Z. et al. (2008) The extracellular matrix of the spleen as a potential organizer of immune cell compartments.
Semin Immunol. 20: 4-13.
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