ER-TR7 antibody | ER-TR7

Rat anti Mouse ER-TR7

Product Type
Monoclonal Antibody
Clone
ER-TR7
Isotype
IgG2a
Specificity
ER-TR7

Product Code Applications Pack Size List Price Your Price Qty
MCA2402
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SDS Safety Datasheet SDS
C F * IF 0.25 mg loader
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Rat anti Mouse ER-TR7 antibody, clone ER-TR7 recognizes ER-TR7, an antigen located in the cytoplasm of reticular fibroblasts, a component of the extracellular matrix of lymphoid and non-lymphoid organs.

Rat anti Mouse ER-TR7 antibody, clone ER-TR7 recognizes Collagen type VI (Schiavinato et al. 2021) and has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Isolated C3H thymic stromal cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Spleen

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Source Reference

  1. VanVliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
    Eur J Immunol. 14 (6): 524-9.

References for ER-TR7 antibody

  1. Van Vliet, E. et al. (1986) Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
    J Histochem Cytochem. 34 (7): 883-90.
  2. Kalled, S.L. et al. (1998) Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function.
    J Immunol. 160: 2158-65.
  3. Köhler, C.N. (2010) The actin-binding protein caldesmon is in spleen and lymph nodes predominately expressed by smooth-muscle cells, reticular cells, and follicular dendritic cells.
    J Histochem Cytochem. 58 (2): 183-93.
  4. Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
    Blood. 116: 3456-64.
  5. Kumamoto, Y. et al. (2009) MGL2 Dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo.
    PLoS One. 4: e5619.
  6. Fujii, N. et al. (2006) Targeting of interstitial cells using a simple gene-transfer strategy.
    Nephrol Dial Transplant. 21: 2745-53.
  7. Katakai, T. et al. (2003) Th1-biased tertiary lymphoid tissue supported by CXC chemokine ligand 13-producing stromal network in chronic lesions of autoimmune gastritis.
    J Immunol. 171: 4359-68.
  8. Mueller, S.N. et al. (2007) Viral targeting of fibroblastic reticular cells contributes to immunosuppression and persistence during chronic infection.
    Proc Natl Acad Sci U S A. 104:15430-5.
  9. View The Latest Product References
  10. Bennett, K.M. et al. (2016) Induction of Colonic M Cells during Intestinal Inflammation.
    Am J Pathol. 186 (5): 1166-79.
  11. Burrell, B.E. et al. (2015) Lymph Node Stromal Fiber ER-TR7 Modulates CD4+ T Cell Lymph Node Trafficking and Transplant Tolerance.
    Transplantation. 99 (6): 1119-25.
  12. Umemoto, E. et al. (2012) Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling.
    J Immunol. 189 (1): 191-9.
  13. Watanabe, R. et al. (2016) Formation of fibroblastic reticular network in the brain after infection with neurovirulent murine coronavirus.
    Neuropathology. Apr 28. [Epub ahead of print]
  14. Marrero, L. et al. (2017) Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
    Regeneration (Oxf). 4 (2): 69-84.
  15. Dawson, L.A. et al. (2020) Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration.
    Wound Repair Regen. Aug 19 [Epub ahead of print].

Further Reading

  1. Lokmic, Z. et al. (2008) The extracellular matrix of the spleen as a potential organizer of immune cell compartments.
    Semin Immunol. 20: 4-13.
  2. Schiavinato, A. et al. (2021) Collagen type VI is the antigen recognized by the ER-TR7 antibody.
    Eur J Immunol. 51 (9): 2345-7.

Flow Cytometry

Immunofluorescence

Synonyms
Collagen alpha-1(VI) Chain
RRID
AB_915429
UniProt
Q04857
Entrez Gene
Col6a1
GO Terms
GO:0007155 cell adhesion
GO:0005578 proteinaceous extracellular matrix
GO:0042383 sarcolemma
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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