Dectin-1 antibody | 2A11
Rat ani Mouse Dectin 1 antibody, clone 2A11 (MCA2289) used for the evaluation of Dectin-1 expression on neutrophils by flow cytometry.
Image caption:
Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a−/−) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/Mø. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean±95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (‘C’) and the use of different fungal particle used (‘F’) were assessed by Two-way ANOVA (‘I’ = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.
From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289) used for the evaluation of dectin-1 expression on wild type and knockout mouse macrophages by flow cytometry.
Image caption:
Dectin-1 response and expression is Ae2-dependent.
(A and B) Tnfα mRNA expression relative to Hprt in Ae2wt/wt and Ae2Δ/Δ macrophages stimulated for 6h with Zymosan (5 ppc) (A) or PAM3CK4 (B) (n = 8–10, ** p<0.01, Mann-Whitney test). (C) Ae2 mRNA expression relative to Hprt by WT macrophages stimulated for 6h with Zymosan (Zym, 5 ppc) or PAM3CK4 (100 ng/ml) (n = 8–12, * p<0.05, *** p<0.001, Mann-Whitney test). (D) Representative flow cytometry histogram with quantification by mean fluorescence intensity (MFI) of Dectin-1 expression on cell surface of Ae2wt/wt and Ae2Δ/Δ macrophages (n = 8, *** p<0.001, Mann-Whitney test). (E) Representative flow cytometry histogram and MFI of CD64 expression on cell surface of Ae2wt/wt and Ae2Δ/Δ macrophages (n = 5).
From: Urso K, Charles JF, Shull GE, Aliprantis AO, Balestrieri B (2016)
Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS ONE 11(7): e0158893.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289) used for the evaluation of dectin-1 expression on wild type and treated mouse macrophages by flow cytometry.
Image caption:
Pharmacological alkalinization of pHi inhibits Dectin-1 expression.
(A) Dectin-1 expression relative to Hprt by wild-type macrophages treated with bafilomycin A (Baf A, 25 nM) or DMSO control for 6 and 16 h. (B) Representative flow cytometry histogram and (C) quantification by MFI showing decreased Dectin-1 in wild-type macrophages treated with Baf A for 6 and 16 h compared to DMSO control (n = 6, ** p<0.01, Mann-Whitney test).
From: Urso K, Charles JF, Shull GE, Aliprantis AO, Balestrieri B (2016)
Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS ONE 11(7): e0158893.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Dectin-1 antibody, clone 2A11 (MCA2289) used for the evaluation of surface dectin-1 expression by murine peritoneal and bone marrow derived macrophages by flow cytometry.
Image caption:
Murine primary macrophages including peritoneal macrophages and BMDM express similar levels of the BG receptor dectin-1 and exhibit the same ability to respond to Sc BG extracts.
(A-B) Freshly isolated 4-days thioglycollate-elicited peritoneal macrophages (TEPM), resident peritoneal macrophages (RPM) and BMDM from WT and Clec7a-/- C57Bl/6, and DBA/2 mice, and the macrophages cell line RAW-Blue, used as control, were assessed for surface expression of dectin-1 by flow cytometry (MACSQuant®, Miltenyi Biotech, Germany). Doublets of cells were excluded at the beginning of the acquisition and a 7-AAD staining was used to discriminate death cells. The surface expression of dectin-1 was determined for each type of primary macrophages on CD115+ cells. (A) Data are expressed as the frequency of dectin-1+ among CD115+ cells. (B) The mean of fluorescence intensity (MFI) of dectin-1 was established on CD115+ cells. Results are presented as the mean ± SD MFI of dectin-1 corrected by either the MFI of isotype control for RAW macrophages, or the MFI of Clec7a-/- C57Bl/6 for WT C57Bl/6 or DBA/2 primary macrophages. (C) Remaining TEPM and BMDM from WT and Clec7a-/- C57Bl/6 mice isolated from these experiments were subcultured and stimulated with 100 μg/mL of crude or enriched Sc BG CW extracts (BG15, BG65 or BG75) or zymosan for 8 h (37°C, 5% CO2). Supernatants were collected immediately after incubation and were assessed for TNFα production by ELISA. Results are shown as the mean ± SD. All data are representative of three mice per group from three independent experiments performed in triplicate. *p <0.05 (two-tailed unpaired Student's t-test); mean values not sharing the same letter are significantly different.
From: Walachowski S, Tabouret G, Foucras G (2016) Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages. PLoS ONE 11(2): e0148464.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289F) used to label RAW264.7 cells to evaluate dectin-1 expression by flow cytometry.
Image caption:
(A) WT RAW264.7 cells transfected with GFP-tagged dectin-1 (green) were infected with mCherry-expressing M. marinum (red) as for Fig. 4A. Bar, 5μm. Data are means ± SD (n = 3). (B) No difference in subcellular localization of CD45 between WT and Sptlc2−/− cells. Wild-type and Sptlc2−/− RAW264.7 cells transfected with GFP-tagged dectin-1 (green) were incubated with zymosan A particles (blue) on ice, warmed to 37°C for 2 min to initiate phagocytosis, fixed, and then stained with antibodies against CD45 (purple). Boxed areas are magnified at right. Arrowheads mark sites of zymosan A binding. Bar, 5μm. (C) WT and Sptlc2−/− RAW264.7 cells were stained with APC-conjugated anti-CD45 antibody, fixed, and subjected to flow cytometry analysis. (D) Quantitative analysis of median fluorescence intensity of cells treated as for panel C. (E) Wild-type and Sptlc2−/− RAW264.7 cells were stained with FITC-conjugated anti-dectin-1 antibody, fixed, and subjected to flow cytometry analysis. (F) Quantitative analysis of median fluorescence intensity of cells treated as for panel E. (G) Immunoblot analysis of total cell lysates collected from wild-type and two independent UGCG−/− KO clones of THP-1 cells using antibodies against UGCG and GAPDH.
From:Niekamp P, Guzman G, Leier HC, Rashidfarrokhi A, Richina V, Pott F, Barisch C, Holthuis JCM, Tafesse FG.
Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis Host Cell Entry.
mBio. 2021 Jan 26;12(1):e03141-20.
doi: 10.1128/mBio.03141-20.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289) used to demonstrate dectin-1 expression in murine brain by immunofluorescence.
Image caption:
Confirmation of several genes in Figure 1E by immunohistochemistry. Lumbar spinal cord sections of symptomatic mutant SOD1 mice (SOD1G37R or SOD1G85R) and control mice were immunostained. (A) Astrogliosis was revealed by GFAP immunostaining (red) in symptomatic mutant SOD1 mice, and oncostatin M receptor (green) encoded by Osmr was mainly upregulated in GFAP-positive astrocytes. (B) Calponin-3 (green) encoded by Cnn3 was mainly upregulated in GFAP-positive astrocytes (red) in symptomatic mutant SOD1 mice. (C) C-type lectin domain family 7 member A (green, alternative name; Dectin-1) encoded by Clec7a was mainly upregulated in Iba1-positive microglia (red) in symptomatic mutant SOD1 mice. Microgliosis was evident in the symptomatic stage.
From: Yamashita H, Komine O, Fujimori-Tonou N, Yamanaka K.
Comprehensive expression analysis with cell-type-specific transcriptome in ALS-linked mutant SOD1 mice: Revisiting the active role of glial cells in disease.
Front Cell Neurosci. 2023 Jan 4;16:1045647.
doi: 10.3389/fncel.2022.1045647.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Dectin-1
- Product Type
- Monoclonal Antibody
- Clone
- 2A11
- Isotype
- IgG2b
- Specificity
- Dectin-1
Antibody Quality and Performance Guarantee
| Rat anti Mouse dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as dectin-1 or CD369. Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Fischer rats were fused with cells of the rat Y3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |
- 1The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Source Reference
-
Brown, G.D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.
J Exp Med. 196 (3): 407-12.
References for Dectin-1 antibody
-
Taylor, P.R. et al. (2002) The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.
J Immunol. 169 (7): 3876-82. -
Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.
J Leukoc Biol. 76 (1): 86-94. -
Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.
Blood. 106 (7): 2543-50. -
Lefevre,L. et al. (2010) PPARc Ligands Switched High Fat Diet-Induced Macrophage M2b Polarization toward M2a Thereby Improving Intestinal Candida Elimination
PLoS One. 5(9):e12828. -
Fei, M. et al. (2011) TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection.
Proc Natl Acad Sci U S A. 108 (13): 5360-5. -
Gazi, U. et al. (2011) Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement.
J Biol Chem. 286: 7822-9. -
McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
FASEB J. 25 (6): 1972-82. -
Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4: e689.
View The Latest Product References
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Galès, A. et al. (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.
PLoS Pathog. 6: e1000714. -
Goodridge, H.S. et al. (2009) Differential use of CARD9 by dectin-1 in macrophages and dendritic cells.
J Immunol. 182: 1146-54. -
Coates, P.J. et al. (2008) Indirect macrophage responses to ionizing radiation: implications for genotype-dependent bystander signaling.
Cancer Res. 68: 450-6. -
Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
J Immunol. 181: 6514-24. -
Hohl, T.M. (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure.
J Infect Dis. 198: 176-85. -
Palma, A.S. et al. (2006) Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides.
J Biol Chem. 281: 5771-9. -
Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
J Clin Invest. 121: 985-97. -
Anandasabapathy, N. et al. (2011) Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain.
J Exp Med. 208 (8): 1695-705. -
Takahara, K. et al. (2012) Efficient capture of Candida albicans and zymosan by SIGNR1 augments TLR2-dependent TNF-α production.
Int Immunol. 24 (2): 89-96. -
Fransen F et al. (2015) BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.
Immunity. 43 (3): 527-40. -
Urso, K. et al. (2016) Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS One. 11 (7): e0158893. -
Zhou, J. et al. (2016) Therapeutic targeting of myeloid-derived suppressor cells involves a novel mechanism mediated by clusterin.
Sci Rep. 6: 29521. -
Pinke, K.H. et al. (2016) Mast cells phagocyte Candida albicans and produce nitric oxide by mechanisms involving TLR2 and Dectin-1.
Immunobiology. 221 (2): 220-7. -
Berven, L. et al. (2015) Particulate yeast β-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain
Bioactive Carbohydrates and Dietary Fibre. 6 (1): 15-23. -
Walachowski, S. et al. (2016) Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages.
PLoS One. 11 (2): e0148464. -
Ferguson, B.J. et al. (2015) The Schistosoma mansoni T2 ribonuclease omega-1 modulates inflammasome-dependent IL-1β secretion in macrophages.
Int J Parasitol. 45 (13): 809-13. -
Masuda, Y. et al. (2015) Soluble β-glucan from Grifola frondosa induces tumor regression in synergy with TLR9 agonist via dendritic cell-mediated immunity.
J Leukoc Biol. 98 (6): 1015-25. -
Baldwin, K.T. et al. (2015) Neuroinflammation triggered by β-glucan/dectin-1 signaling enables CNS axon regeneration.
Proc Natl Acad Sci U S A. 112 (8): 2581-6. -
Quayle K et al. (2015) The TLR2 agonist in polysaccharide-K is a structurally distinct lipid which acts synergistically with the protein-bound β-glucan.
J Nat Med. 69 (2): 198-208. -
Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
PLoS Pathog. 13 (7): e1006485. -
Seo, B.S. et al. (2016) Dectin-1 agonist selectively induces IgG1 class switching by LPS-activated mouse B cells.
Immunol Lett. 178: 114-21. -
Thompson, A. et al. (2019) The protective effect of inflammatory monocytes during systemic C. albicans. infection is dependent on collaboration between C-type lectin-like receptors.
PLoS Pathog. 15 (6): e1007850. -
Uno, A. et al. (2021) A novel β-glucan-oligonucleotide complex selectively delivers siRNA to APCs via Dectin-1.
J Control Release. 338: 792-803. -
Deerhake, M.E. et al. (2021) Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M.
Immunity. 54 (3): 484-498.e8. -
Niekamp, P. et al. (2021) Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis. Host Cell Entry.
mBio. 12 (1): e03141-20. -
Oh, S. et al. (2022) Pathogen size alters C-type lectin receptor signaling in dendritic cells to influence CD4 Th9 cell differentiation.
Cell Rep. 38 (13): 110567. -
Masuda, Y. et al. (2023) Maitake α-glucan promotes differentiation of monocytic myeloid-derived suppressor cells into M1 macrophages.
Life Sci. 317: 121453. -
Yamashita, H. et al. (2022) Comprehensive expression analysis with cell-type-specific transcriptome in ALS-linked mutant SOD1 mice: Revisiting the active role of glial cells in disease.
Front Cell Neurosci. 16: 1045647. -
Erkelens, M.N. et al. (2020) Intestinal Macrophages Balance Inflammatory Expression Profiles via Vitamin A and Dectin-1-Mediated Signaling.
Front Immunol. 11: 551. -
Zimara, N. et al. (2018) Dectin-1 Positive Dendritic Cells Expand after Infection with Leishmania major Parasites and Represent Promising Targets for Vaccine Development.
Front Immunol. 9: 263.
- Synonyms
- CD369
- RRID
- AB_324904
- UniProt
- Q6QLQ4
- Entrez Gene
- Clec7a
- GO Terms
- GO:0001846 opsonin binding
- GO:0001874 1,3-beta-D-glucan receptor activity
- GO:0001879 detection of yeast
- GO:0002238 response to molecule of fungal origin
- GO:0002752 cell surface pattern recognition receptor signaling pathway
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- View More GO Terms
- GO:0006954 inflammatory response
- GO:0009897 external side of plasma membrane
- GO:0016337 cell-cell adhesion
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0050766 positive regulation of phagocytosis
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