Dectin-1 antibody | 2A11
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|Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.
As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line
- For research purposes only
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
|Immunohistology - Resin|
- 1 The epitope recognized by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for Dectin-1 antibody
Goodridge, H.S. et al. (2009) Differential use of CARD9 by dectin-1 in macrophages and dendritic cells.
J Immunol. 182: 1146-54.
Brown, G.D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.
J Exp Med. 196 (3): 407-12.
Taylor, P.R. et al. (2002) The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.
J Immunol. 169 (7): 3876-82.
Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.
J Leukoc Biol. 76 (1): 86-94.
Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.
Blood. 106 (7): 2543-50.
Lefevre,L. et al. (2010) PPARc Ligands Switched High Fat Diet-Induced Macrophage M2b Polarization toward M2a Thereby Improving Intestinal Candida Elimination
PLoS One. 5(9):e12828.
Fei, M. et al. (2011) TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection.
Proc Natl Acad Sci U S A. 108 (13): 5360-5.
Gazi, U. et al. (2011) Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement.
J Biol Chem. 286: 7822-9.
View The Latest Product References
McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
FASEB J. 25 (6): 1972-82.
Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4: e689.
Galès, A. et al. (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.
PLoS Pathog. 6: e1000714.
Coates, P.J. et al. (2008) Indirect macrophage responses to ionizing radiation: implications for genotype-dependent bystander signaling.
Cancer Res. 68: 450-6.
Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
J Immunol. 181: 6514-24.
Hohl, T.M. (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure.
J Infect Dis. 198: 176-85.
Palma, A.S. et al. (2006) Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides.
J Biol Chem. 281: 5771-9.
Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
J Clin Invest. 121: 985-97.
Anandasabapathy, N. et al. (2011) Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain.
J Exp Med. 208 (8): 1695-705.
Takahara, K. et al. (2012) Efficient capture of Candida albicans and zymosan by SIGNR1 augments TLR2-dependent TNF-α production.
Int Immunol. 24 (2): 89-96.
Fransen F et al. (2015) BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.
Immunity. 43 (3): 527-40.
Urso, K. et al. (2016) Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS One. 11 (7): e0158893.
Zhou, J. et al. (2016) Therapeutic targeting of myeloid-derived suppressor cells involves a novel mechanism mediated by clusterin.
Sci Rep. 6: 29521.
Pinke, K.H. et al. (2016) Mast cells phagocyte Candida albicans and produce nitric oxide by mechanisms involving TLR2 and Dectin-1.
Immunobiology. 221 (2): 220-7.
Berven, L. et al. (2015) Particulate yeast β-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain
Bioactive Carbohydrates and Dietary Fibre. 6 (1): 15-23.
Walachowski, S. et al. (2016) Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages.
PLoS One. 11 (2): e0148464.
Ferguson, B.J. et al. (2015) The Schistosoma mansoni T2 ribonuclease omega-1 modulates inflammasome-dependent IL-1β secretion in macrophages.
Int J Parasitol. 45 (13): 809-13.
Masuda, Y. et al. (2015) Soluble β-glucan from Grifola frondosa induces tumor regression in synergy with TLR9 agonist via dendritic cell-mediated immunity.
J Leukoc Biol. 98 (6): 1015-25.
Baldwin, K.T. et al. (2015) Neuroinflammation triggered by β-glucan/dectin-1 signaling enables CNS axon regeneration.
Proc Natl Acad Sci U S A. 112 (8): 2581-6.
Quayle K et al. (2015) The TLR2 agonist in polysaccharide-K is a structurally distinct lipid which acts synergistically with the protein-bound β-glucan.
J Nat Med. 69 (2): 198-208.
Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
PLoS Pathog. 13 (7): e1006485.
Seo, B.S. et al. (2016) Dectin-1 agonist selectively induces IgG1 class switching by LPS-activated mouse B cells.
Immunol Lett. 178: 114-21.
Thompson, A. et al. (2019) The protective effect of inflammatory monocytes during systemic C. albicans. infection is dependent on collaboration between C-type lectin-like receptors.
PLoS Pathog. 15 (6): e1007850.
Uno, A. et al. (2021) A novel β-glucan-oligonucleotide complex selectively delivers siRNA to APCs via Dectin-1.
J Control Release. 338: 792-803.
Deerhake, M.E. et al. (2021) Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M.
Immunity. 54 (3): 484-498.e8.
Niekamp, P. et al. (2021) Sphingomyelin Biosynthesis Is Essential for Phagocytic Signaling during Mycobacterium tuberculosis. Host Cell Entry.
mBio. 12 (1): e03141-20.
Oh, S. et al. (2022) Pathogen size alters C-type lectin receptor signaling in dendritic cells to influence CD4 Th9 cell differentiation.
Cell Rep. 38 (13): 110567.
- Entrez Gene
- GO Terms
- GO:0001846 opsonin binding
- GO:0001874 1,3-beta-D-glucan receptor activity
- GO:0001879 detection of yeast
- GO:0002238 response to molecule of fungal origin
- GO:0002752 cell surface pattern recognition receptor signaling pathway
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- View More GO Terms
- GO:0006954 inflammatory response
- GO:0009897 external side of plasma membrane
- GO:0016337 cell-cell adhesion
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0050766 positive regulation of phagocytosis
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