Dectin-1 antibody | 2A11

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Rat anti Mouse Dectin-1:Alexa Fluor® 647

Rat anti Mouse Dectin-1:Biotin

Rat anti Mouse Dectin-1:FITC

Rat anti Mouse Dectin-1:Low Endotoxin

Rat anti Mouse Dectin-1

Rat anti Mouse Dectin-1:RPE

Product Type
Monoclonal Antibody
Clone
2A11
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2289A647T F 25 Tests/0.25ml
MCA2289A647 F 100 Tests/1ml
MCA2289B F 0.1 mg
MCA2289BT F 25 µg
MCA2289F F 0.1 mg
MCA2289FA F 50 µg
MCA2289FB F 0.5 mg
MCA2289FT F 25 µg
MCA2289EL C* F FN IP 0.5 mg
MCA2289GA C* F IP 0.1 mg
MCA2289T C* F IP 25 µg
MCA2289 C* F IP 0.25 mg
MCA2289PET F 25 Tests
MCA2289PE F 100 Tests
Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.

As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.

Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 25 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute with 0.25ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg, 50 µg, 25 µg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 0.1 mg, 0.5 mg, 25 µg
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 50 µg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of reconstitution.

More Information

UniProt
Q6QLQ4 Related reagents
Entrez Gene
Clec7a Related reagents
GO Terms
GO:0001846 opsonin binding
GO:0001874 1,3-beta-D-glucan receptor activity
GO:0001879 detection of yeast
GO:0002238 response to molecule of fungal origin
GO:0002752 cell surface pattern recognition receptor signaling pathway
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006954 inflammatory response
GO:0009897 external side of plasma membrane
GO:0016337 cell-cell adhesion
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0050766 positive regulation of phagocytosis
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Dectin-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat Pack Size: 0.1 mg, 25 µg
1/5 Pack Size: 0.5 mg
1/10 Pack Size: 0.1 mg, 25 µg
1/50 Pack Size: 0.5 mg
Flow Cytometry 1/10 1/50
Functional Assays
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Flow Cytometry Neat 1/10
  1. 1The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  1. 1The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Functional Assays

Immunofluorescence

Product Specific References

References for Dectin-1 antibody

  1. Brown, G.D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages.
    J Exp Med. 196 (3): 407-12.
  2. Taylor, P.R. et al. (2002) The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.
    J Immunol. 169 (7): 3876-82.
  3. Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions.
    J Leukoc Biol. 76 (1): 86-94.
  4. Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.
    Blood. 106 (7): 2543-50.
  5. Lefevre,L. et al. (2010) PPARc Ligands Switched High Fat Diet-Induced Macrophage M2b Polarization toward M2a Thereby Improving Intestinal Candida Elimination
    PLoS One. 5(9):e12828.
  6. Fei, M. et al. (2011) TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection.
    Proc Natl Acad Sci U S A. 108 (13): 5360-5.
  7. Gazi, U. et al. (2011) Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement.
    J Biol Chem. 286: 7822-9.
  8. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
    FASEB J. 25 (6): 1972-82.
  9. Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
    PLoS Negl Trop Dis. 4: e689.
  10. Galès, A. et al. (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.
    PLoS Pathog. 6: e1000714.
  11. Coates, P.J. et al. (2008) Indirect macrophage responses to ionizing radiation: implications for genotype-dependent bystander signaling.
    Cancer Res. 68: 450-6.
  12. Dioszeghy, V. et al. (2008) 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo.
    J Immunol. 181: 6514-24.
  13. Hohl, T.M. (2008) Caspofungin modulates inflammatory responses to Aspergillus fumigatus through stage-specific effects on fungal beta-glucan exposure.
    J Infect Dis. 198: 176-85.
  14. Palma, A.S. et al. (2006) Ligands for the beta-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides.
    J Biol Chem. 281: 5771-9.
  15. Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
    J Clin Invest. 121: 985-97.
  16. Anandasabapathy, N. et al. (2011) Flt3L controls the development of radiosensitive dendritic cells in the meninges and choroid plexus of the steady-state mouse brain.
    J Exp Med. 208 (8): 1695-705.
  17. Takahara, K. et al. (2012) Efficient capture of Candida albicans and zymosan by SIGNR1 augments TLR2-dependent TNF-α production.
    Int Immunol. 24 (2): 89-96.
  18. Fransen F et al. (2015) BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.
    Immunity. 43 (3): 527-40.
  19. Urso, K. et al. (2016) Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
    PLoS One. 11 (7): e0158893.
  20. Zhou, J. et al. (2016) Therapeutic targeting of myeloid-derived suppressor cells involves a novel mechanism mediated by clusterin.
    Sci Rep. 6: 29521.
  21. Pinke, K.H. et al. (2016) Mast cells phagocyte Candida albicans and produce nitric oxide by mechanisms involving TLR2 and Dectin-1.
    Immunobiology. 221 (2): 220-7.
  22. Berven, L. et al. (2015) Particulate yeast β-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain
    Bioactive Carbohydrates and Dietary Fibre. 6 (1): 15-23.
  23. Walachowski, S. et al. (2016) Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages.
    PLoS One. 11 (2): e0148464.
  24. Ferguson, B.J. et al. (2015) The Schistosoma mansoni T2 ribonuclease omega-1 modulates inflammasome-dependent IL-1β secretion in macrophages.
    Int J Parasitol. 45 (13): 809-13.
  25. Masuda, Y. et al. (2015) Soluble β-glucan from Grifola frondosa induces tumor regression in synergy with TLR9 agonist via dendritic cell-mediated immunity.
    J Leukoc Biol. 98 (6): 1015-25.
  26. Baldwin, K.T. et al. (2015) Neuroinflammation triggered by β-glucan/dectin-1 signaling enables CNS axon regeneration.
    Proc Natl Acad Sci U S A. 112 (8): 2581-6.
  27. Quayle K et al. (2015) The TLR2 agonist in polysaccharide-K is a structurally distinct lipid which acts synergistically with the protein-bound β-glucan.
    J Nat Med. 69 (2): 198-208.
  28. Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
    PLoS Pathog. 13 (7): e1006485.
  29. Seo, B.S. et al. (2016) Dectin-1 agonist selectively induces IgG1 class switching by LPS-activated mouse B cells.
    Immunol Lett. 178: 114-21.