CD81 antibody | Eat2










Hamster anti Mouse CD81:Alexa Fluor® 488
Hamster anti Mouse CD81:Alexa Fluor® 647
Hamster anti Mouse CD81:FITC
Hamster anti Mouse CD81
Hamster anti Mouse CD81:RPE
- Product Type
- Monoclonal Antibody
- Clone
- Eat2
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1846A488T | F | 25 Tests/0.25ml |
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MCA1846A488 | F | 100 Tests/1ml |
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MCA1846A647 | F | 100 Tests/1ml |
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MCA1846F | F | 0.1 mg |
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MCA1846T | C* E F IP WB* | 25 µg |
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MCA1846GA | C* E F IP WB* | 0.1 mg |
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MCA1846 | C* E F IP WB* | 0.25 mg |
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MCA1846PE | F | 100 Tests |
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Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- 38C13, murine B cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 0.25 mgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 0.25 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35762
- Entrez Gene
- Cd81
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0008104 protein localization
- GO:0005887 integral to plasma membrane
- GO:0006661 phosphatidylinositol biosynthetic process
- GO:0030307 positive regulation of cell growth
- GO:0030890 positive regulation of B cell proliferation
- GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD81 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | Neat Pack Size: 25 Tests/0.25ml 1/2 Pack Size: 100 Tests/1ml |
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Flow Cytometry | Neat | ||
Flow Cytometry | |||
ELISA | |||
Flow Cytometry | 1/50 Pack Size: 25 µg, 0.25 mg 1/10 Pack Size: 0.1 mg |
1/100 Pack Size: 25 µg, 0.25 mg 1/50 Pack Size: 0.1 mg |
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Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting 2 | |||
Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2Clone Eat2 recognizes mouse CD81 under non-reducing conditions.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C).- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
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Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | F | 0.4 mg |
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Goat anti Hamster IgG:Biotin | STAR79B | E F | 0.8 mg |
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Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
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Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
References for CD81 antibody
-
Clark, K.L. et al. (2001) PGRL is a major CD81-associated protein on lymphocytes and distinguishes a new family of cell surface proteins.
J Immunol. 167 (9): 5115-21. -
Maecker, H.T. et al. (2000) Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies.
Hybridoma 19: 15-22. -
Conde-Vancells, J. et al. (2010) Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Proteomics Clin Appl. 4 (4): 416-25. -
Conde-Vancells, J. et al. (2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.
J Proteome Res. 7: 5157-66. -
Takeda, Y. et al. (2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.
J Biol Chem. 283: 26089-97. -
Suzuki, M. et al. (2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.
J Immunol. 182: 6485-93. -
Ha, C.T. et al. (2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.
J Leukoc Biol. 77: 948-57. -
Pan, Q. et al. (2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).
Gut. 61: 1330-9. -
Jin, Y. et al. (2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.
PLoS One. 8: e73706. -
Royo, F. et al. (2013) Transcriptome of extracellular vesicles released by hepatocytes.
PLoS One. 8: e68693. -
Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.
Cancer Res. 61: 5248-54. -
Jin, Y. et al. (2018) Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.
Sci Rep. 8 (1): 5145. -
Sosa, L.J. et al. (2013) Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.
PLoS One. 8 (5): e64521.
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