CD8 Alpha antibody | KT15
Rat anti Mouse CD4 antibody, clone KT15 (MCA609) used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide boost leads to loss of high avidity responses in vivo. A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFNγ elispot assay, D) normalization of responses to increasing peptide concentration in IFNγ elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments.
From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes in a mouse model of colorectal liver metastasis by immunohistochemistry and immunofluorescence on acetone treated liver cryosections.
Image caption:
(a) Immunohistochemistry and (b) immunofluorescence double staining: Representative image of CD4 (red)/CD8 (blue) (top) expression and Foxp3 (FITC)/CD4 (Cy3) (bottom) expression in liver metastases on day 10 (n = 7) compared to increased CD4 (red)/CD8 (blue) expression and Foxp3 (FITC)/CD4 (Cy3) expression on day 20. Immunohistochemistry: Nova red brick red color, Vector Blue blue color. Haemalaun blue color-nuclear counterstaining. Asterisk with indication lines show metastases next to normal tissue (×100 and ×400). Immunofluorescence: FITC green fluorescein isothiocyanate, Cy3 red and DAPI 4′,6-diamidino-2-phenylindole blue - nuclear counterstaining (×250).
From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A. Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases. BMC Cancer. 2010 Mar 7;10:82.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes in a mouse model of colorectal liver metastasis by immunofluorescence on acetone treated liver cryosections.
Image caption:
Immunofluorescence: Representative images of increased CD8+/FAS (a) and CD8+/TUNEL (b) expression on tumor infiltrating lymphocytes at the margin of liver metastases on day 20. FITC green Fluorescein isothiocyanate, Cy3 red and DAPI 4′,6-Diamidino-2-phenylindoldihydrochlorid blue - nuclear countersaining. Case demonstrates area of the TUNEL staining in serial cryostat sections. To adjust a better contrast TUNEL stained sections were not counterstained with DAPI. Asterisk with indication line shows metastatic tumor cells. Magnifications ×250 (top) and ×400 (bottom).
From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A. Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases. BMC Cancer. 2010 Mar 7;10:82.
Rat anti Mouse CD8α antibody, clone KT15 use for the detection of CD8 positive T cells from tumors by flow cytometry and immunofluorescence.
Image caption:
Vaccination with pFap suppresses TAM, MDSC, Treg, and enhances DC and CTL recruitment. Primary tumors were isolated from mice 25 days after orthotopic challenge and immune cells were identified in primary tumors using antibodies for the following: (A) tumor-associated macrophages (TAMs): F4/80 (red), (B) myeloid derived suppressor cells (MDSCs): CD11b (red, nuclear) and Gr-1 (green, nuclear), (C) T regulatory cells (Tregs, white arrowheads): CD4 (red, cell surface) and FOXP3 (green, nuclear), (D) dendritic cells (DC): 33D1 (red), and (E) cytotoxic T lymphocytes (CTL): CD8 (red) Scale bar for all panels=100 μm.
From: Liao D, Luo Y, Markowitz D, Xiang R, Reisfeld RA (2009) Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model. PLoS ONE 4(11): e7965.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
sIM-1.6.α induces enduring CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Lymphocytes were stimulated in vitro with PPD (50 &mu:g/ml) for 48 h and later stained with fluorochrome conjugated antibodies and analyzed by flowcytometry. A, flow contours depict percentage of gated CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD4 T cells (lower panel). C, gated CD8 T cells expressing IL-7R and CD44 (upper panel) and CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. "*" and "**" indicate p<0.05 and p<0.01 respectively.
From: Singh V, Jain S, Gowthaman U, Parihar P, Gupta P, et al. (2011) Co-Administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis Infected Macrophages Vaccine Induces Better Protective T Cell Memory than BCG. PLoS ONE 6(1): e16097.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Vaccination with xIM-1.6.α induces CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Pooled lymphocytes from spleen and lymphnodes of immunized animals were stimulated in vitro with PPD (50 μg/ml) for 48 h and later stained with fluorochrome tagged antibodies and analyzed by flowcytometry. A, flow contours depict percentage of CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L expression on CD4 T cells (lower panel). C, CD8 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. "*" indicates p<0.05.
From: Singh V, Jain S, Gowthaman U, Parihar P, Gupta P, et al. (2011) Co-Administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis Infected Macrophages Vaccine Induces Better Protective T Cell Memory than BCG. PLoS ONE 6(1): e16097.
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide immunization induces low avidity functionally impaired CTL. Splenocytes from mice immunized with peptide or DNA were analyzed at day 20 for A, Presence of antigen specific CTL by pentamer and CD8 staining. B, Frequency of epitope specific responses in IFNγ elispot assay C, Avidity and frequency of epitope specific responses by measuring responses to increasing peptide concentration in IFNγ elispot assay, D, Analysis of the avidity by normalization of responses. E, The ability of CTL lines to lyse tumor cells in chromium release assay. Data is representative of at least three independent experiments.
From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide boost leads to loss of high avidity responses in vivo. A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFNγ elispot assay, D) normalization of responses to increasing peptide concentration in IFNγ elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments.
From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012) High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.
Rat anti Mouse CD8 Alpha:Alexa Fluor® 488
Rat anti Mouse CD8 Alpha:Alexa Fluor® 647
Rat anti Mouse CD8 Alpha:APC
Rat anti Mouse CD8 Alpha:FITC
Rat anti Mouse CD8 Alpha:Pacific Blue®
Rat anti Mouse CD8 Alpha
Rat anti Mouse CD8 Alpha:RPE
Rat anti Mouse CD8 Alpha:RPE-Alexa Fluor® 647
Rat anti Mouse CD8 Alpha:RPE-Alexa Fluor® 750
Rat anti Mouse CD8 Alpha:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- KT15
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA609A488 | F | 100 Tests/1ml |
|
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| MCA609A647T | F | 25 Tests/0.25ml |
|
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| MCA609A647 | F | 100 Tests/1ml |
|
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| MCA609APCT | F | 25 Tests |
|
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| MCA609APC | F | 100 Tests |
|
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| MCA609F | F | 0.1 mg |
|
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| MCA609FB | F | 0.5 mg |
|
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| MCA609PBT | F | 25 Tests/0.25ml |
|
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| MCA609PB | F | 100 Tests/1ml |
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| MCA609GT | C* F IF | 25 µg |
|
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| MCA609GA | C* F IF | 0.1 mg |
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| MCA609G | C* F IF | 0.25 mg |
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| MCA609PE | F | 100 Tests |
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| MCA609P647 | F | 100 Tests/1ml |
|
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| MCA609P750 | F | 100 Tests/1ml |
|
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| MCA609A700 | F | 100 Tests/1ml |
|
Rat anti mouse CD8α, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8α and CD8β chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).
Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).
Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Pack Size: 25 TestsReconstitute with 0.25ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- T cell clone, C6
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised SD rats were fused with cells of the NS0 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 0.25 mgStore at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 18 months from date of despatch
More Information
- UniProt
- P01731 Related reagents
- Entrez Gene
- Cd8a Related reagents
- GO Terms
- GO:0002456 T cell mediated immunity
- GO:0016021 integral to membrane
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0042803 protein homodimerization activity
- GO:0045065 cytotoxic T cell differentiation
- GO:0050850 positive regulation of calcium-mediated signaling
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat Pack Size: 0.1 mg 1/50 Pack Size: 0.5 mg |
1/10 Pack Size: 0.1 mg 1/100 Pack Size: 0.5 mg |
|
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | 1/50 Pack Size: 25 µg, 0.25 mg 1/100 Pack Size: 0.1 mg |
1/100 Pack Size: 25 µg, 0.25 mg 1/200 Pack Size: 0.1 mg |
|
| Immunofluorescence | |||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Alexa Fluor® 488 | MCA1212A488 | F | 100 Tests/1ml |
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| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
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| Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
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| Rat IgG2a Negative Control:Pacific Blue® | MCA1212PB | F | 100 Tests/1ml |
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| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
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| Rat IgG2a Negative Control:RPE-Alexa Fluor® 647 | MCA1212P647 | F | 100 Tests/1ml |
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| Rat IgG2a Negative Control:Alexa Fluor® 700 | MCA1212A700 | F | 100 Tests/1ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Application Based External Images
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for CD8 Alpha antibody
-
Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
Immunogenetics. 28 (6): 445-51. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Lee, Y.L. et al (2003) Oral administration of Agaricus blazei (H1 strain) inhibited tumor growth in a sarcoma
180 inoculation model.
Exp Anim. 52: 371-5. -
Eller, K. et al. (2011) IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression.
J Immunol. 186: 83-91. -
Grimm, M. et al. (2010) Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
BMC Cancer. 10: 82. -
Liao, D. et al. (2009) Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model
PLoS One. 4: e7965. -
Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice.
Arterioscler Thromb Vasc Biol. 25: 2386-91. -
Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
J Gen Virol. 83: 1735-43. -
Wang, X. et al. (2011) Quercetin and Bornyl Acetate Regulate T-Lymphocyte Subsets and INF-γ/IL-4 Ratio In Utero in Pregnant Mice.
Evid Based Complement Alternat Med. 2011: 745262. -
Zeis, M. et al. (2002) Idiotype protein-pulsed dendritic cells produce strong anti-myeloma effects after syngeneic stem cell transplantation in mice.
Bone Marrow Transplant. 29: 213-21. -
Ideguchi, M. et al. (2008) Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell-derived neural precursor cells.
J Neurosci Res. 86: 1936-43. -
Wolf, D. et al. (2005) CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice.
J Am Soc Nephrol. 16: 1360-70. -
Severinova, J. et al. (2005) Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.
Folia Microbiol (Praha). 50: 457-63. -
Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
J Clin Invest. 117: 3330-8. -
Meyer, C. et al. (2011) Chronic inflammation promotes myeloid-derived suppressor cell activation blocking antitumor immunity in transgenic mouse melanoma model.
Proc Natl Acad Sci U S A. 108: 17111-6. -
Zitt, E. et al. (2011) The selective mineralocorticoid receptor antagonist eplerenone is protective in mild anti-GBM glomeru-lonephritis.
Int J Clin Exp Pathol. 4:606-15. -
Singh, V. et al. (2011) Co-administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis infected macrophages vaccine induces better protective T cell memory than BCG.
PLoS One. 6: e16097. -
Kalyanasundaram Bhanumathy, K. et al. (2015) Potent immunotherapy against well-established thymoma using adoptively transferred transgene IL-6-engineered dendritic cell-stimulated CD8(+) T-cells with prolonged survival and enhanced cytotoxicity.
J Gene Med. 17 (8-9): 153-60. -
Abiko K et al. (2015) IFN-γ from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer.
Br J Cancer. 112 (9): 1501-9. -
Phan-Lai, V. et al. (2016) The Antitumor Efficacy of IL2/IL21-Cultured Polyfunctional Neu-Specific T Cells Is TNFα/IL17 Dependent.
Clin Cancer Res. 22 (9): 2207-16. -
Kajiwara, T. et al. (2016) Hypoxia augments MHC class I antigen presentation via facilitation of ERO1-α-mediated oxidative folding in murine tumor cells.
Eur J Immunol. Sep 26. [Epub ahead of print] -
Srivastava, A.K. et al. (2016) Co-transplantation of syngeneic mesenchymal stem cells improves survival of allogeneic glial-restricted precursors in mouse brain.
Exp Neurol. 275 Pt 1: 154-61.
