CD8 Alpha antibody | KT15
Rat anti Mouse CD4 antibody, clone KT15 (MCA609) used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide boost leads to loss of high avidity responses in vivo. A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFNγ elispot assay, D) normalization of responses to increasing peptide concentration in IFNγ elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments.
From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012)
High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment.
PLoS ONE 7(7): e41112.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes in a mouse model of colorectal liver metastasis by immunohistochemistry and immunofluorescence on acetone treated liver cryosections.
Image caption:
(a) Immunohistochemistry and (b) immunofluorescence double staining: Representative image of CD4 (red)/CD8 (blue) (top) expression and Foxp3 (FITC)/CD4 (Cy3) (bottom) expression in liver metastases on day 10 (n = 7) compared to increased CD4 (red)/CD8 (blue) expression and Foxp3 (FITC)/CD4 (Cy3) expression on day 20. Immunohistochemistry: Nova red brick red color, Vector Blue blue color. Haemalaun blue color-nuclear counterstaining. Asterisk with indication lines show metastases next to normal tissue (×100 and ×400). Immunofluorescence: FITC green fluorescein isothiocyanate, Cy3 red and DAPI 4′,6-diamidino-2-phenylindole blue - nuclear counterstaining (×250).
From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A.
Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
BMC Cancer. 2010 Mar 7;10:82.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes in a mouse model of colorectal liver metastasis by immunofluorescence on acetone treated liver cryosections.
Image caption:
Immunofluorescence: Representative images of increased CD8+/FAS (a) and CD8+/TUNEL (b) expression on tumor infiltrating lymphocytes at the margin of liver metastases on day 20. FITC green Fluorescein isothiocyanate, Cy3 red and DAPI 4′,6-Diamidino-2-phenylindoldihydrochlorid blue - nuclear countersaining. Case demonstrates area of the TUNEL staining in serial cryostat sections. To adjust a better contrast TUNEL stained sections were not counterstained with DAPI. Asterisk with indication line shows metastatic tumor cells. Magnifications ×250 (top) and ×400 (bottom).
From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A.
Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
BMC Cancer. 2010 Mar 7;10:82.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD8α antibody, clone KT15 use for the detection of CD8 positive T cells from tumors by flow cytometry and immunofluorescence.
Image caption:
Vaccination with pFap suppresses TAM, MDSC, Treg, and enhances DC and CTL recruitment. Primary tumors were isolated from mice 25 days after orthotopic challenge and immune cells were identified in primary tumors using antibodies for the following: (A) tumor-associated macrophages (TAMs): F4/80 (red), (B) myeloid derived suppressor cells (MDSCs): CD11b (red, nuclear) and Gr-1 (green, nuclear), (C) T regulatory cells (Tregs, white arrowheads): CD4 (red, cell surface) and FOXP3 (green, nuclear), (D) dendritic cells (DC): 33D1 (red), and (E) cytotoxic T lymphocytes (CTL): CD8 (red) Scale bar for all panels=100 μm.
From: Liao D, Luo Y, Markowitz D, Xiang R, Reisfeld RA (2009)
Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model.
PLoS ONE 4(11): e7965.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
sIM-1.6.α induces enduring CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Lymphocytes were stimulated in vitro with PPD (50 &mu:g/ml) for 48 h and later stained with fluorochrome conjugated antibodies and analyzed by flowcytometry. A, flow contours depict percentage of gated CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD4 T cells (lower panel). C, gated CD8 T cells expressing IL-7R and CD44 (upper panel) and CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. "*" and "**" indicate p<0.05 and p<0.01 respectively.
From: Singh V, Jain S, Gowthaman U, Parihar P, Gupta P, et al. (2011)
Co-Administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis Infected Macrophages Vaccine Induces Better Protective T Cell Memory than BCG.
PLoS ONE 6(1): e16097.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Vaccination with xIM-1.6.α induces CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Pooled lymphocytes from spleen and lymphnodes of immunized animals were stimulated in vitro with PPD (50 μg/ml) for 48 h and later stained with fluorochrome tagged antibodies and analyzed by flowcytometry. A, flow contours depict percentage of CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L expression on CD4 T cells (lower panel). C, CD8 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. "*" indicates p<0.05.
From: Singh V, Jain S, Gowthaman U, Parihar P, Gupta P, et al. (2011)
Co-Administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis Infected Macrophages Vaccine Induces Better Protective T Cell Memory than BCG.
PLoS ONE 6(1): e16097.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide immunization induces low avidity functionally impaired CTL. Splenocytes from mice immunized with peptide or DNA were analyzed at day 20 for A, Presence of antigen specific CTL by pentamer and CD8 staining. B, Frequency of epitope specific responses in IFNγ elispot assay C, Avidity and frequency of epitope specific responses by measuring responses to increasing peptide concentration in IFNγ elispot assay, D, Analysis of the avidity by normalization of responses. E, The ability of CTL lines to lyse tumor cells in chromium release assay. Data is representative of at least three independent experiments.
From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012)
High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD4 antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.
Image caption:
Peptide boost leads to loss of high avidity responses in vivo. A, schematic of immunization regime. Mice immunized with DNA were boosted at day 63 with DNA or peptide, or with peptide at day 42 followed by DNA at day 63. Responses were analyzed at day 70 for frequency (B) and avidity (C) by measuring responses to increasing peptide concentration in IFNγ elispot assay, D) normalization of responses to increasing peptide concentration in IFNγ elispot assay. E, Analysis of antigen specific CTL by pentamer and CD8 staining of immunized splenocytes. F, Analysis of memory phenotype of antigen specific CTL by combination staining for CD62L and CD127 markers. Data is representative of at least three independent experiments.
From: Brentville VA, Metheringham RL, Gunn B, Durrant LG (2012)
High Avidity Cytotoxic T Lymphocytes Can Be Selected into the Memory Pool but They Are Exquisitely Sensitive to Functional Impairment. PLoS ONE 7(7): e41112.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse CD8 alpha antibody, clone YTS191.1 (MCA609G) used to identify CD8 T lymphocytes in murine optic nerve tissue by immunofluorescence.
Image caption:
Therapeutic fingolimod and teriflunomide treatment attenuates neuroinflammation in CLN1 mice.
(A) Representative immune fluorescence microscopy and quantification of CD8+ and (B) CD4+ T-lymphocytes in longitudinal optic nerve sections from 7-month-old Ppt1+/+ , Ppt1-/-, fingolimod-treated Ppt1-/- (+ FTY720) and teriflunomide-treated Ppt1-/- (+ Teriflunomide) mice (circle = mean value of one mouse; n = 5 mice per group, One-way ANOVA and Tukey's post hoc test). Scale bars: 20 μm.
From: Janos Groh, Kristina Berve, Rudolf Martini,
Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset,
Brain Communications, 2021;, fcab047,
doi: 10.1093/braincomms/fcab047
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD8 antibody, clone KT15 (MCA609G) used to identify CD8 expressing cells in mouse brain by immunohistochemistry on cryosections.
Image caption:
CD28SA reduces neuroinflammation in the nigrostriatal tract of hαSyn PD mice. Immunohistochemical stainings and quantification for CD8+ (D–F). Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: SN CD8+, F(3, 35) = 9.74, P < 0.0001; Striatum CD8+ count analyzed by Kruskal–Wallis test followed by Dunn’s multiple comparisons test, H = 10.56, P = 0.0144. *P < 0.05, **P < 0.01, ***P < 0.001. All data are shown as mean ± SEM. n = number of biologically independent animals. Scale bars: 20 μm each
From: Badr M, McFleder RL, Wu J, Knorr S, Koprich JB, Hünig T, Brotchie JM, Volkmann J, Lutz MB, Ip CW.
Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice.
J Neuroinflammation. 2022 Dec 31;19(1):319.
doi: 10.1186/s12974-022-02685-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD8 antibody, clone KT15 (MCA609G) used to identify CD8 expressing cells in mouse brain by immunohistochemistry on cryosections.
Image caption:
Adoptive transfer of Treg diminishes neuroinflammation in the nigrostriatal tract of hαSyn PD mice; CD28SA decreases early T cell activation in brain and modulates early IL-2 response in peripheral lymphatic organs of hαSyn PD mice; delayed treatment with CD28SA fails to reduce SN dopaminergic neurodegeneration in hαSyn PD mice. A-D Immunohistochemical stainings and quantification for CD4+ (A, B) and CD8+ (C, D) T cells in the SN and striatum of hαSyn-injected mice with either PBS or Treg delivery. Statistical analysis by unpaired two-tailed t-test: SN CD4+: t(14) = 2.367, P = 0.0329; striatum CD4+: t(14) = 2.601, P = 0.0209; SN CD8+: t(14) = 2.835, P = 0.0132; striatum CD8+: t(14) = 5.2, P = 0.0001. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars: 20 μm each.
From: Badr M, McFleder RL, Wu J, Knorr S, Koprich JB, Hünig T, Brotchie JM, Volkmann J, Lutz MB, Ip CW.
Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice.
J Neuroinflammation. 2022 Dec 31;19(1):319.
doi: 10.1186/s12974-022-02685-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD8 Alpha
- Product Type
- Monoclonal Antibody
- Clone
- KT15
- Isotype
- IgG2a
- Specificity
- CD8 Alpha
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD8α, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8α and CD8β chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987). Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- T cell clone, C6
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized SD rats were fused with cells of the NS0 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 Pack Size: 0.25 mg, 25 µg 1/100 Pack Size: 0.1 mg |
1/100 Pack Size: 0.25 mg, 25 µg 1/200 Pack Size: 0.1 mg |
| Immunofluorescence | |
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| Immunohistology - Frozen 1 | |
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| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD8 Alpha antibody
-
Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
Immunogenetics. 28 (6): 445-51. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Lee, Y.L. et al (2003) Oral administration of Agaricus blazei (H1 strain) inhibited tumor growth in a sarcoma
180 inoculation model.
Exp Anim. 52: 371-5. -
Eller, K. et al. (2011) IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression.
J Immunol. 186: 83-91. -
Grimm, M. et al. (2010) Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
BMC Cancer. 10: 82. -
Liao, D. et al. (2009) Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model
PLoS One. 4: e7965. -
Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice.
Arterioscler Thromb Vasc Biol. 25: 2386-91. -
Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
J Gen Virol. 83: 1735-43.
View The Latest Product References
-
Wang, X. et al. (2011) Quercetin and Bornyl Acetate Regulate T-Lymphocyte Subsets and INF-γ/IL-4 Ratio In Utero in Pregnant Mice.
Evid Based Complement Alternat Med. 2011: 745262. -
Zeis, M. et al. (2002) Idiotype protein-pulsed dendritic cells produce strong anti-myeloma effects after syngeneic stem cell transplantation in mice.
Bone Marrow Transplant. 29: 213-21. -
Ideguchi, M. et al. (2008) Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell-derived neural precursor cells.
J Neurosci Res. 86: 1936-43. -
Wolf, D. et al. (2005) CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice.
J Am Soc Nephrol. 16: 1360-70. -
Severinova, J. et al. (2005) Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.
Folia Microbiol (Praha). 50: 457-63. -
Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
J Clin Invest. 117: 3330-8. -
Meyer, C. et al. (2011) Chronic inflammation promotes myeloid-derived suppressor cell activation blocking antitumor immunity in transgenic mouse melanoma model.
Proc Natl Acad Sci U S A. 108: 17111-6. -
Zitt, E. et al. (2011) The selective mineralocorticoid receptor antagonist eplerenone is protective in mild anti-GBM glomeru-lonephritis.
Int J Clin Exp Pathol. 4:606-15. -
Singh, V. et al. (2011) Co-administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis infected macrophages vaccine induces better protective T cell memory than BCG.
PLoS One. 6: e16097. -
Kalyanasundaram Bhanumathy, K. et al. (2015) Potent immunotherapy against well-established thymoma using adoptively transferred transgene IL-6-engineered dendritic cell-stimulated CD8(+) T-cells with prolonged survival and enhanced cytotoxicity.
J Gene Med. 17 (8-9): 153-60. -
Abiko K et al. (2015) IFN-γ from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer.
Br J Cancer. 112 (9): 1501-9. -
Phan-Lai, V. et al. (2016) The Antitumor Efficacy of IL2/IL21-Cultured Polyfunctional Neu-Specific T Cells Is TNFα/IL17 Dependent.
Clin Cancer Res. 22 (9): 2207-16. -
Kajiwara, T. et al. (2016) Hypoxia augments MHC class I antigen presentation via facilitation of ERO1-α-mediated oxidative folding in murine tumor cells.
Eur J Immunol. 46 (12): 2842-51. -
Srivastava, A.K. et al. (2016) Co-transplantation of syngeneic mesenchymal stem cells improves survival of allogeneic glial-restricted precursors in mouse brain.
Exp Neurol. 275 Pt 1: 154-61. -
Meier, R.P. et al. (2014) Survival of free and encapsulated human and rat islet xenografts transplanted into the mouse bone marrow.
PLoS One. 9 (3): e91268. -
Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
Brain Communications. 3(2): fcab047. -
Cecil, D.L. et al. (2022) COX-2 inhibitors decrease expression of PD-L1 in colon tumors and increase the influx of Type I tumor infiltrating lymphocytes.
Cancer Prev Res (Phila). canprevres.0227.2021. -
Karikari, A.A. et al. (2022) Neurodegeneration by α-synuclein-specific T cells in AAV-A53T-α-synuclein Parkinson's disease mice.
Brain Behav Immun. 101: 194-210. -
Badr, M. et al. (2022) Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice.
J Neuroinflammation. 19 (1): 319. -
McFleder, R.L. et al. (2023) Brain-to-gut trafficking of alpha-synuclein by CD11c(+) cells in a mouse model of Parkinson's disease.
Nat Commun. 14 (1): 7529. -
Aloui, A. et al. (2023) AFM1 Exposure in Male Balb/c Mice and Intervention Strategies Against Its Immuno-physiological toxicity using Clay Mineral and Lactic Acid Bacteria Alone or in Combination.
Immunopharmacol Immunotoxicol. : 1-32. -
Aringer, I. et al. (2021) Agonism of Prostaglandin E2 Receptor 4 Ameliorates Tubulointerstitial Injury in Nephrotoxic Serum Nephritis in Mice.
J Clin Med. 10 (4) :832.
- Synonyms
- LY-2
- RRID
- AB_321407
- UniProt
- P01731
- Entrez Gene
- Cd8a
- GO Terms
- GO:0002456 T cell mediated immunity
- GO:0016021 integral to membrane
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0042803 protein homodimerization activity
- GO:0045065 cytotoxic T cell differentiation
- GO:0050850 positive regulation of calcium-mediated signaling
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