CD69 antibody | H1.2F3

Hamster anti Mouse CD69

Product Type
Monoclonal Antibody
Clone
H1.2F3
Isotype
IgG
Specificity
CD69

Product Code Applications Pack Size List Price Your Price Qty
MCA1580R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Hamster anti Mouse CD69 antibody, clone H1.2F3 recognizes the murine CD69 cell surface antigen, an ~85 kDa dimeric glycoprotein. CD69 is expressed rapidly on the surface of T cells, B cells and NK cells following activation.

Target Species
Mouse
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Murine dendritic epidermal Y245 cell line.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2 1/20
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.

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References for CD69 antibody

  1. Yokoyama, W.M. et al. (1988) Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.
    J Immunol. 141 (2): 369-76.
  2. Hickman, H.D. et al. (2008) Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
    Nat Immunol. 9 (2): 155-65.
  3. Chalifour, A. et al. (2004) Direct bacterial protein PAMP recognition by human NK cells involves TLRs and triggers alpha-defensin production.
    Blood. 104 (6): 1778-83.
  4. Cohen-sfady, M. et al. (2005) Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway.
    J Immunol. 175 (6): 3594-602.
  5. Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
    Proc Natl Acad Sci U S A. 98 (16): 9226-30.
  6. Karrer, U. et al. (2003) Memory inflation: continuous accumulation of antiviral CD8+ T cells over time.
    J Immunol. 170 (4): 2022-9.
  7. Kvakan, H. et al. (2009) Regulatory T cells ameliorate angiotensin II-induced cardiac damage.
    Circulation. 119 (22): 2904-12.
  8. Marshall-clarke, S. et al. (2003) A differential requirement for phosphoinositide 3-kinase reveals two pathways for inducible upregulation of major histocompatibility complex class II molecules and CD86 expression by murine B lymphocytes.
    Immunology. 109 (1): 102-8.
  9. View The Latest Product References

Flow Cytometry

Synonyms
AIM
RRID
AB_2260066
UniProt
P37217
Entrez Gene
Cd69
GO Terms
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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