CD69 antibody | H1.2F3

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Hamster anti Mouse CD69:FITC

Hamster anti Mouse CD69

Hamster anti Mouse CD69:RPE

Product Type
Monoclonal Antibody
Clone
H1.2F3
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1580FT F 0.1 mg
MCA1580R C F IP 0.2 mg
MCA1580PE F 100 Tests/0.5ml
Hamster anti Mouse CD69 antibody, clone H1.2F3 recognizes the murine CD69 cell surface antigen, an ~85 kDa dimeric glycoprotein. CD69 is expressed rapidly on the surface of T cells, B cells and NK cells following activation.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Murine dendritic epidermal Y245 cell line.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P37217 Related reagents
Entrez Gene
Cd69 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
Regulatory
For research purposes only

Applications of CD69 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/2 1/20
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F 0.1 mg F
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD69 antibody

  1. Yokoyama, W.M. et al. (1988) Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.
    J Immunol. 141 (2): 369-76.
  2. Hickman, H.D. et al. (2008) Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
    Nat Immunol. 9 (2): 155-65.
  3. Chalifour, A. et al. (2004) Direct bacterial protein PAMP recognition by human NK cells involves TLRs and triggers alpha-defensin production.
    Blood. 104 (6): 1778-83.
  4. Cohen-sfady, M. et al. (2005) Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway.
    J Immunol. 175 (6): 3594-602.
  5. Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
    Proc Natl Acad Sci U S A. 98 (16): 9226-30.
  6. Karrer, U. et al. (2003) Memory inflation: continuous accumulation of antiviral CD8+ T cells over time.
    J Immunol. 170 (4): 2022-9.
  7. Kvakan, H. et al. (2009) Regulatory T cells ameliorate angiotensin II-induced cardiac damage.
    Circulation. 119 (22): 2904-12.
  8. Marshall-clarke, S. et al. (2003) A differential requirement for phosphoinositide 3-kinase reveals two pathways for inducible upregulation of major histocompatibility complex class II molecules and CD86 expression by murine B lymphocytes.
    Immunology. 109 (1): 102-8.
  9. Zajac, A.J. et al. (1998) Viral immune evasion due to persistence of activated T cells without effector function.
    J Exp Med. 188 (12): 2205-13.
  10. Everitt, A.R. et al. (2012) IFITM3 restricts the morbidity and mortality associated with influenza.
    Nature. 484: 519-23.