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CD45R antibody | RA3-6B2

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Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 1 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 2 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 3 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 4 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 5 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 6 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 7 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 8 Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 9
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 1
Figure A. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and Amethyst Orange conjugated Rat IgG2a isotype control (MCA6005AMO). Figure B. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and Amethyst Orange conjugated Rat anti Mouse CD45R (MCA1258AMO). All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 2
Published customer image:
Rat anti Mouse CD45R antibody, clone RA3-6B2 (MCA1258) used for the enumeration of B cells in the intestinal mucosa by immunofluorescence.
Image caption:
Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 μg/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistochemistry. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.

From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F (2009)
Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation.
PLoS ONE 4(12): e8291.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 3
Figure A. Alexa647® conjugated hamster anti mouse CD79b (MCA1821A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. Alexa647® conjugated hamster anti mouse CD79b (MCA1821A647) and RPE conjugated rat anti mouse CD45R (MCA1258PE). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 4
Figure A. RPE conjugated mouse anti mouse CD28 (MCA2473PE) and Alexa647® conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated mouse anti mouse CD28 (MCA2473PE) and Alexa647® conjugated rat anti mouse CD45 (MCA1258A647). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 5
Figure A. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat IgG2a isotype control (MCA1212A488). Figure B. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat anti Mouse CD45R (MCA1258A488). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 6
Published customer image:
Rat anti Mouse CD45R antibody, clone RA3-6B2 (MCA1258G) used for the identification of marginal zone B cells in mouse spleen by immunofluorescence on cryostat sections.
Image caption:
Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.

From: Zhou Z, Niu H, Zheng YY, Morel L.
Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 2011 Jan 20;12:7.
This image is from an open access article distributed under the terms of Creative Commons Attribution License.
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 7
Published customer image:
Rat anti MouseCD45R antibody, clone RA3-6B2 (MCA1258G) used for the demonstration of B cells in murine spinal column by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative images of key immunohistochemical markers run on a longitudinally sectioned, decalcified spinal column from an EAE mouse with a clinical EAE score of 4.
(A) HE, (B) B220 immunohistochemistry for (B) cells, (C) F4/80 immunohistochemistry for macrophages (arrow indicates area highlighted in inset, inset bar = 20 μm), (D) CD3 immunohistochemistry for T cells. Bars = 200 μm.

From: Gibson-Corley KN, Boyden AW, Leidinger MR, Lambertz AM, Ofori-Amanfo G, Naumann PW, Goeken JA, Karandikar NJ.
A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.
PeerJ. 2016 Jan 26;4:e1600.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 8
Figure A. FITC conjugated Rat anti Mouse CD19 (MCA1439F). Figure B. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and SBV515 conjugated Rat anti Mouse CD45R (MCA1258SBV515). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 9
Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD45R (MCA1258A488). Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD45R (MCA1258A488) and SBV515 conjugated Rat anti Mouse CD19 (MCA1439SBV515). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.

Rat anti Mouse CD45R:Alexa Fluor® 488

Rat anti Mouse CD45R:Alexa Fluor® 647

Rat anti Mouse CD45R:FITC

Rat anti Mouse CD45R

Rat anti Mouse CD45R:RPE

Rat anti Mouse CD45R:RPE-Alexa Fluor® 647

Rat anti Mouse CD45R:RPE-Alexa Fluor® 750

Rat anti Mouse CD45R:RPE-Cy7

Rat anti Mouse CD45R:Alexa Fluor® 700

Rat anti Mouse CD45R:Starbright Blue 700

Rat anti Mouse CD45R:Amethyst Orange

Rat anti Mouse CD45R:Starbright Violet 515

Product Type
Monoclonal Antibody
Clone
RA3-6B2
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1258A488T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA1258A488
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1258A647T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA1258A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1258F
Datasheet
F
MSDS
0.1 mg loader

MCA1258GT
Datasheet
C F IP P*
MSDS
25 µg loader

MCA1258G
Datasheet
C F IF IP P*
MSDS
0.25 mg loader

MCA1258PE
Datasheet
F
MSDS
100 Tests loader

MCA1258P647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1258P750
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1258PECY7
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1258A700
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1258SBB700
Datasheet
F
MSDS
100 Tests/0.5ml loader

MCA1258AMO
Datasheet
F
MSDS
0.1 mg loader

MCA1258SBV515
Datasheet
F
MSDS
100 Tests/0.5ml loader

Rat anti Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220 kDa).

Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin -Cy7 (RPE-Cy7) - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Product Form
Purified IgG conjugated to StarBright Blue 700 - liquid
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Product Form
Purified IgG conjugated to StarBright Violet 515 - liquid
Reconstitution
Reconstitute with 1 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Stabilizing agent
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
Carrier Free
Yes
Immunogen
Murine leukemia-induced pre-B tumor cells (RAW112)
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P06800
Entrez Gene
Ptprc
GO Terms
GO:0000187 activation of MAPK activity
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0051726 regulation of cell cycle
GO:0004725 protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007159 leukocyte cell-cell adhesion
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0031953 negative regulation of protein autophosphorylation
GO:0034113 heterotypic cell-cell adhesion
GO:0042098 T cell proliferation
GO:0042100 B cell proliferation
GO:0043065 positive regulation of apoptosis
GO:0043395 heparan sulfate proteoglycan binding
GO:0043410 positive regulation of MAPKKK cascade
GO:0045059 positive thymic T cell selection
GO:0045060 negative thymic T cell selection
GO:0045121 membrane raft
GO:0045577 regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050855 regulation of B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
Acknowledgements
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Regulatory
For research purposes only

Applications of CD45R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry 1/100 1/200
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry neat 1/5
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
  1. 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C).
Flow Cytometry
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 488 MCA1212A488 F 100 Tests/1ml loader
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 F 100 Tests/1ml loader
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader
Rat IgG2a Negative Control:RPE-Alexa Fluor® 647 MCA1212P647 F 100 Tests/1ml loader
Rat IgG2a Negative Control:Alexa Fluor® 700 MCA1212A700 F 100 Tests/1ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD45R antibody

  1. Coffman, R.L. (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
    Immunol Rev. 69: 5-23.
  2. Rosmalen, J.G. et al. (2000) Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes.
    Lab Invest. 80 (1): 23-30.
  3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  4. Spangrude, G.J. et al. (1988) Purification and characterization of mouse hematopoietic stem cells.
    Science. 241: 58-62.
  5. Spangrude, G.J. et al. (1988) Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus.
    J Exp Med. 167 (5): 1671-83.
  6. Holmes, K.L. et al. (1986) Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts.
    J Immunol. 137 (2): 679-88.
  7. Ankeny, D.P. et al. (2009) B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice.
    J Clin Invest. 119: 2990-9.
  8. Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
    Infect Immun. 78 (5): 1924-30.
  9. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
    Infect Immun. 74: 4841-8.
  10. Kleiter, I. et al. (2010) Smad7 in T cells drives T helper 1 responses in multiple sclerosis and experimental autoimmune encephalomyelitis.
    Brain. 133: 1067-81.
  11. Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
    PLoS One. 4: e8291.
  12. Bertilaccio, M.T. et al. (2011) Lack of TIR8/SIGIRR triggers progression of chronic lymphocytic leukemia in mouse models.
    Blood. 118: 660-9.
  13. Gengozian, N. et al. (2005) Characterization of a monoclonal antibody identifying a CD45RA antigen on feline leukocytes.
    Vet Immunol Immunopathol. 108: 253-64.
  14. Giuriato, S. et al. (2010) Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.
    Blood. 115: 4061-70.
  15. Hawke, S. et al. (1998) Long-term persistence of activated cytotoxic T lymphocytes after viral infection of the central nervous system.
    J Exp Med. 187: 1575-82.
  16. Nakaya, T. et al. (2010) Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression.
    J Immunol. 185: 5180-7.
  17. Perry, M.J. et al. (2000) Effects of high-dose estrogen on murine hematopoietic bone marrow precede those on osteogenesis.
    Am J Physiol Endocrinol Metab. 279: E1159-65.
  18. Gibson-Corley, K.N. et al. (2016) A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.
    PeerJ. 4: e1600.
  19. Soejima, M. et al. (2011) Role of innate immunity in a murine model of histidyl-transfer RNA synthetase (Jo-1)-mediated myositis.
    Arthritis Rheum. 63: 479-87.
  20. Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
    J Gen Virol. 83: 1735-43.
  21. Fanning, S. et al. (2012) Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection.
    Proc Natl Acad Sci U S A. 109 (6): 2108-13.
  22. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
    Antimicrob Agents Chemother. 56: 687-96.
  23. Carpenter, R.S. et al. (2015) Traumatic spinal cord injury in mice with human immune systems.
    Exp Neurol. 271: 432-44.
  24. Lastrucci, C. et al. (2015) Molecular and cellular profiles of the resolution phase in a damage-associated molecular pattern (DAMP)-mediated peritonitis model and revelation of leukocyte persistence in peritoneal tissues.
    FASEB J. 29 (5): 1914-29.
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