CD45R antibody | RA3-6B2

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Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 1

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 2

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 3

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 4

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 5

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 6

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 7

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 8

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 9

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 10

Anti Mouse CD45R Antibody, clone RA3-6B2 thumbnail image 11

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 1 — Figure A. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and Amethyst Orange conjugated Rat IgG2a isotype control (MCA6005AMO). Figure B. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and Amethyst Orange conjugated Rat anti Mouse CD45R (**MCA1258AMO**). All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 2 — **Published customer image:**
Rat anti Mouse CD45R antibody, clone RA3-6B2 (**MCA1258**) used for the enumeration of B cells in the intestinal mucosa by immunofluorescence.
**Image caption:**
Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 μg/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistochemistry. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.

From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F (2009)
Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation.
PLoS ONE 4(12): e8291.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 3 — Figure A. Alexa647® conjugated hamster anti mouse CD79b (MCA1821A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. Alexa647® conjugated hamster anti mouse CD79b (MCA1821A647) and RPE conjugated rat anti mouse CD45R (**MCA1258PE**). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 4 — Figure A. RPE conjugated mouse anti mouse CD28 (MCA2473PE) and Alexa647® conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated mouse anti mouse CD28 (MCA2473PE) and Alexa647® conjugated rat anti mouse CD45 (**MCA1258A647**). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 5 — Figure A. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat IgG2a isotype control (MCA1212A488). Figure B. RPE conjugated Rat anti Mouse CD36 (MCA2748PE) and A488 conjugated Rat anti Mouse CD45R (**MCA1258A488**). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 6 — **Published customer image:**
Rat anti Mouse CD45R antibody, clone RA3-6B2 (**MCA1258G**) used for the identification of marginal zone B cells in mouse spleen by immunofluorescence on cryostat sections.
**Image caption:**
Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.

From: Zhou Z, Niu H, Zheng YY, Morel L.
Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
BMC Immunol. 2011 Jan 20;12:7.
This image is from an open access article distributed under the terms of Creative Commons Attribution License.

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 7 — Figure A. FITC conjugated Rat anti Mouse CD19 (MCA1439F). Figure B. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and SBV515 conjugated Rat anti Mouse CD45R (**MCA1258SBV515**). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 8 — Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD45R **(MCA1258A488**). Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD45R (**MCA1258A488**) and SBV515 conjugated Rat anti Mouse CD19 (MCA1439SBV515). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 9 — **Published customer image:**
Rat anti MouseCD45R antibody, clone RA3-6B2 (**MCA1258G**) used for the demonstration of B cells in murine spinal column by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
**Image caption:**
Representative images of key immunohistochemical markers run on a longitudinally sectioned, decalcified spinal column from an EAE mouse with a clinical EAE score of 4.
(A) HE, (B) B220 immunohistochemistry for (B) cells, (C) F4/80 immunohistochemistry for macrophages (arrow indicates area highlighted in inset, inset bar = 20 μm), (D) CD3 immunohistochemistry for T cells. Bars = 200 μm.

From: Gibson-Corley KN, Boyden AW, Leidinger MR, Lambertz AM, Ofori-Amanfo G, Naumann PW, Goeken JA, Karandikar NJ.
A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.
PeerJ. 2016 Jan 26;4:e1600.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 10 — Figure A. FITC conjugated Rat anti Mouse CD19 (MCA1439F). Figure B. FITC conjugated Rat anti Mouse CD19 (MCA1439F) and SBV440 conjugated Rat anti Mouse CD45R (**MCA1258SBV440**). All experiments performed on mouse splenocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.

Anti Mouse CD45R Antibody, clone RA3-6B2 gallery image 11 — Figure A. StarBright Blue 700 conjugated Rat anti Mouse CD45R (**MCA1258SBB700**). Figure B. StarBright Blue 700 conjugated Rat anti Mouse CD45R (**MCA1258SBB700**) and StarBright Violet 610 conjugated Rat anti Mouse CD19 (MCA1439SBV610). All experiments performed on red blood lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.

Rat anti Mouse CD45R:Alexa Fluor® 488

Rat anti Mouse CD45R:Alexa Fluor® 647

Rat anti Mouse CD45R:FITC

Rat anti Mouse CD45R

Rat anti Mouse CD45R:RPE

Rat anti Mouse CD45R:RPE-Alexa Fluor® 647

Rat anti Mouse CD45R:RPE-Alexa Fluor® 750

Rat anti Mouse CD45R:RPE-Cy7

Rat anti Mouse CD45R:Alexa Fluor® 700

Rat anti Mouse CD45R:Starbright Blue 700

Rat anti Mouse CD45R:Amethyst Orange

Rat anti Mouse CD45R:Starbright Violet 440

Rat anti Mouse CD45R:Starbright Violet 515

Rat anti Mouse CD45R:Starbright Violet 610

Product Type: Monoclonal Antibody
Clone: RA3-6B2
Isotype: IgG2a

Product Code	Applications	Pack Size	Your Price	Quantity
MCA1258A488T Datasheet	F SDS	25 Tests/0.25ml	Log in	Get Quote
MCA1258A488 Datasheet	F SDS	100 Tests/1ml	Log in	Get Quote
MCA1258A647T Datasheet	F SDS	25 Tests/0.25ml	Log in	Get Quote
MCA1258A647 Datasheet	F SDS	100 Tests/1ml	Log in	Get Quote
MCA1258F Datasheet	F SDS	0.1 mg	Log in	Get Quote
MCA1258GT Datasheet	C F IP P* SDS	25 µg	Log in	Get Quote
MCA1258G Datasheet	C F IF IP P* SDS	0.25 mg	Log in	Get Quote
MCA1258PE Datasheet	F SDS	100 Tests	Log in	Get Quote
MCA1258P647 Datasheet	F SDS	100 Tests/1ml	Log in	Get Quote
MCA1258P750 Datasheet	F SDS	100 Tests/1ml	Log in	Get Quote
MCA1258PECY7 Datasheet	F SDS	100 Tests/1ml	Log in	Get Quote
MCA1258A700 Datasheet	F SDS	100 Tests/1ml	Log in	Get Quote
MCA1258SBB700 Datasheet	F SDS	100 Tests/0.5ml	Log in	Get Quote
MCA1258AMO Datasheet	F SDS	0.1 mg	Log in	Get Quote
MCA1258SBV440 Datasheet	F SDS	100 Tests/0.5ml	Log in	Get Quote
MCA1258SBV515 Datasheet	F SDS	100 Tests/0.5ml	Log in	Get Quote
MCA1258SBV610 Datasheet	F SDS	100 Tests/0.5ml	Log in	Get Quote

Rat anti Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220 kDa).

Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Human
Cat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized

Product Form

Purified IgG conjugated to R. Phycoerythrin -Cy7 (RPE-Cy7) - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 700 - liquid

Product Form

Purified IgG conjugated to StarBright Blue 700 - liquid

Product Form

Purified IgG conjugated to Amethyst Orange - liquid

Product Form

Purified IgG conjugated to StarBright Violet 440 - liquid

Product Form

Purified IgG conjugated to StarBright Violet 515 - liquid

Product Form

Purified IgG conjugated to StarBright Violet 610 - liquid

Reconstitution

Reconstitute with 1 ml distilled water

Reconstitution

Reconstitute with 1.0 ml distilled water

Reconstitution

Reconstitute with 1.0 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G.

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)
Stabilizing agent

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20

Carrier Free

Yes

Immunogen

Murine leukemia-induced pre-B tumor cells (RAW112)

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Fusion Partners

Spleen cells from immunized Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC.

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage: Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage: Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: Guaranteed until date of expiry. Please see product label.
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

UniProt: P06800
Entrez Gene: Ptprc
GO Terms: GO:0000187 activation of MAPK activity; GO:0001915 negative regulation of T cell mediated cytotoxicity; GO:0001916 positive regulation of T cell mediated cytotoxicity; GO:0001960 negative regulation of cytokine-mediated signaling pathway; GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin; GO:0051726 regulation of cell cycle; GO:0004725 protein tyrosine phosphatase activity; GO:0005887 integral to plasma membrane; GO:0005925 focal adhesion; GO:0006469 negative regulation of protein kinase activity; GO:0006470 protein dephosphorylation; GO:0007159 leukocyte cell-cell adhesion; GO:0008201 heparin binding; GO:0009897 external side of plasma membrane; GO:0019901 protein kinase binding; GO:0030183 B cell differentiation; GO:0030890 positive regulation of B cell proliferation; GO:0031953 negative regulation of protein autophosphorylation; GO:0034113 heterotypic cell-cell adhesion; GO:0042098 T cell proliferation; GO:0042100 B cell proliferation; GO:0043065 positive regulation of apoptosis; GO:0043395 heparan sulfate proteoglycan binding; GO:0043410 positive regulation of MAPKKK cascade; GO:0045059 positive thymic T cell selection; GO:0045060 negative thymic T cell selection; GO:0045121 membrane raft; GO:0045577 regulation of B cell differentiation; GO:0045588 positive regulation of gamma-delta T cell differentiation; GO:0046641 positive regulation of alpha-beta T cell proliferation; GO:0048304 positive regulation of isotype switching to IgG isotypes; GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation; GO:0050852 T cell receptor signaling pathway; GO:0050853 B cell receptor signaling pathway; GO:0050855 regulation of B cell receptor signaling pathway; GO:0050857 positive regulation of antigen receptor-mediated signaling pathway; GO:0051209 release of sequestered calcium ion into cytosol; GO:0051607 defense response to virus
Acknowledgements: The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Acknowledgements: Cy® and CyDye® are registered trademarks of GE Healthcare
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Regulatory: For research purposes only

Applications of CD45R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	Neat	1/10
Flow Cytometry	Neat	1/10
Flow Cytometry	1/100	1/200
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin ¹
Immunoprecipitation
Flow Cytometry		Neat
Flow Cytometry		Neat
Flow Cytometry	Neat	1/10
Flow Cytometry	neat	1/5
Flow Cytometry		Neat
Flow Cytometry	Neat
Flow Cytometry	Neat
Flow Cytometry	Neat
Flow Cytometry	Neat
Flow Cytometry	Neat

¹PLP fixation is recommended for optimal results, see Whiteland et al. for details

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C).
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 5ul of the suggested working dilution to label 10⁶ cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry: Use 5ul of the suggested working dilution to label 10⁶ cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry: Use 5ul of the suggested working dilution to label 10⁶ cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry: Use 5ul of the suggested working dilution to label 10⁶ cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	C E P WB	1 ml	Log in	Get Quote
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	C E IF P WB	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	C E P RE	1 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	F	0.5 ml	Log in	Get Quote
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	F	0.5 ml	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Rat IgG2a Negative Control:Alexa Fluor® 488	MCA1212A488	F	100 Tests/1ml	Log in	Get Quote
Rat IgG2a Negative Control:Alexa Fluor® 647	MCA1212A647	F	100 Tests/1ml	Log in	Get Quote
Rat IgG2a Negative Control:FITC	MCA1212F	F	100 Tests	Log in	Get Quote
Rat IgG2a Negative Control	MCA1212	E F	1 ml	Log in	Get Quote
Rat IgG2a Negative Control:RPE	MCA1212PE	F	100 Tests	Log in	Get Quote
Rat IgG2a Negative Control:RPE-Alexa Fluor® 647	MCA1212P647	F	100 Tests/1ml	Log in	Get Quote
Rat IgG2a Negative Control:Alexa Fluor® 700	MCA1212A700	F	100 Tests/1ml	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD45R antibody

Coffman, R.L. (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
Immunol Rev. 69: 5-23.
Rosmalen, J.G. et al. (2000) Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes.
Lab Invest. 80 (1): 23-30.
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20.
Spangrude, G.J. et al. (1988) Purification and characterization of mouse hematopoietic stem cells.
Science. 241: 58-62.
Spangrude, G.J. et al. (1988) Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus.
J Exp Med. 167 (5): 1671-83.
Holmes, K.L. et al. (1986) Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts.
J Immunol. 137 (2): 679-88.
Ankeny, D.P. et al. (2009) B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice.
J Clin Invest. 119: 2990-9.
Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
Infect Immun. 78 (5): 1924-30.
Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
Infect Immun. 74: 4841-8.
Kleiter, I. et al. (2010) Smad7 in T cells drives T helper 1 responses in multiple sclerosis and experimental autoimmune encephalomyelitis.
Brain. 133: 1067-81.
Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
PLoS One. 4: e8291.
Bertilaccio, M.T. et al. (2011) Lack of TIR8/SIGIRR triggers progression of chronic lymphocytic leukemia in mouse models.
Blood. 118: 660-9.
Gengozian, N. et al. (2005) Characterization of a monoclonal antibody identifying a CD45RA antigen on feline leukocytes.
Vet Immunol Immunopathol. 108: 253-64.
Giuriato, S. et al. (2010) Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.
Blood. 115: 4061-70.
Hawke, S. et al. (1998) Long-term persistence of activated cytotoxic T lymphocytes after viral infection of the central nervous system.
J Exp Med. 187: 1575-82.
Nakaya, T. et al. (2010) Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression.
J Immunol. 185: 5180-7.
Perry, M.J. et al. (2000) Effects of high-dose estrogen on murine hematopoietic bone marrow precede those on osteogenesis.
Am J Physiol Endocrinol Metab. 279: E1159-65.
Gibson-Corley, K.N. et al. (2016) A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.
PeerJ. 4: e1600.
Soejima, M. et al. (2011) Role of innate immunity in a murine model of histidyl-transfer RNA synthetase (Jo-1)-mediated myositis.
Arthritis Rheum. 63: 479-87.
Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
J Gen Virol. 83: 1735-43.
Fanning, S. et al. (2012) Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection.
Proc Natl Acad Sci U S A. 109 (6): 2108-13.
Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
Antimicrob Agents Chemother. 56: 687-96.
Carpenter, R.S. et al. (2015) Traumatic spinal cord injury in mice with human immune systems.
Exp Neurol. 271: 432-44.
Lastrucci, C. et al. (2015) Molecular and cellular profiles of the resolution phase in a damage-associated molecular pattern (DAMP)-mediated peritonitis model and revelation of leukocyte persistence in peritoneal tissues.
FASEB J. 29 (5): 1914-29.

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CD45R antibody | RA3-6B2

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Storage Information

More Information

Applications of CD45R antibody

Secondary Antibodies Available

Negative Isotype Controls Available

Useful Reagents Available

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD45R antibody

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

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