CD41 antibody | MWReg30

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Rat anti Mouse CD41:Alexa Fluor® 488

Rat anti Mouse CD41:Alexa Fluor® 647

Rat anti Mouse CD41:Biotin

Rat anti Mouse CD41:FITC

Rat anti Mouse CD41

Rat anti Mouse CD41:RPE

Rat anti Mouse CD41:RPE-Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
MWReg30
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2245A488 F 100 Tests/1ml
MCA2245A647 F 100 Tests/1ml
MCA2245B F 0.1 mg
MCA2245F F 0.1 mg
MCA2245T C F IP 25 µg
MCA2245GA C F IP 0.1 mg
MCA2245 C F IP 0.25 mg
MCA2245PET F 25 Tests
MCA2245PE F 100 Tests
MCA2245P647 F 100 Tests
Rat anti Mouse CD41 antibody, clone MWReg30 recognizes the mouse integrin alpha IIb subunit CD41. CD41 is a ~125 kDa single pass type 1 transmembrane glycoprotein expressed by platelets, megakaryocytes (Zhang et al. 2007), mast cells (Berlanga et al. 2005) and hematopoietic progenitors (Mitjavila-Garcia et al. 2002). CD41 forms a heterodimer with CD61. The CD41/CD61 complex is important for platelet adhesion and aggregation (Patel et al. 2003) acting as a receptor for many extracellular matrix proteins including fibronectin, thrombospondin and vitronectin (Weisel et al. 1992).

Rat anti mouse CD41, clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo (Nieswandt et al. 1999).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Purified murine platelets
Approx. Protein Concentrations
Ig concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
Q9QUM0 Related reagents
Entrez Gene
Itga2b Related reagents
GO Terms
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0005925 focal adhesion
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
GO:0050840 extracellular matrix binding
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD41 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B)
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:Alexa Fluor® 488 MCA1211A488 100 Tests/1ml F
Rat IgG1 Negative Control:Alexa Fluor® 647 MCA1211A647 100 Tests/1ml F
Rat IgG1 Negative Control:FITC MCA1211F 100 Tests F
Rat IgG1 Negative Control MCA1211 1 ml C E F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

Source Reference

  1. Nieswandt, B. et al. (1999) Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice.
    Blood. 94 (2): 684-93.

References for CD41 antibody

  1. Winter, O. et al. (2010) Megakaryocytes constitute a functional component of a plasma cell niche in the bone marrow.
    Blood. 116: 1867-75.
  2. Tamagawa-Mineoka, R. et al. (2007) The role of platelets in leukocyte recruitment in chronic contact hypersensitivity induced by repeated elicitation.
    Am J Pathol. 170: 2019-29.
  3. Takayama, M. et al. (2010) Genetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis.
    Mol Cell Biol. 30: 2668-80.
  4. Larson, M.K. and Watson, S.P. (2006) Regulation of proplatelet formation and platelet release by integrin alpha IIb beta3.
    Blood. 108: 1509-14.
  5. Zanzinger, K. et al. (2009) Regulation of triggering receptor expressed on myeloid cells 1 expression on mouse inflammatory monocytes.
    Immunology. 128: 185-95.
  6. Lutskiy, M.I. et al. (2007) WASP localizes to the membrane skeleton of platelets.
    Br J Haematol. 139: 98-105.
  7. Sullivan, B.P. et al. (2010) Protective and damaging effects of platelets in acute cholestatic liver injury revealed by depletion and inhibition strategies.
    Toxicol Sci. 115: 286-94.
  8. Fujita, R. et al. (2013) NF-E2 p45 Is Important for Establishing Normal Function of Platelets.
    Mol Cell Biol. 33: 2659-70.
  9. Perez, L.E. et al. (2008) SH2-inositol phosphatase 1 negatively influences early megakaryocyte progenitors.
    PLoS One. 3: e3565.
  10. Teeling,,J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction
    Acta Neuropathol. 124: 479-90.
  11. Motohashi, H. et al. (2010) NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation.
    Blood. 115 (3): 677-86.
  12. Flierl, U. et al. (2015) Phosphorothioate backbone modifications of nucleotide-based drugs are potent platelet activators.
    J Exp Med. 212 (2): 129-37.
  13. Devanathan, V. et al. (2015) Platelet Gi protein Gαi2 is an essential mediator of thrombo-inflammatory organ damage in mice.
    Proc Natl Acad Sci U S A. 112 (20): 6491-6.
  14. Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.
    Exp Hematol. 43 (10): 891-900.e4.
  15. Goggs, R. et al. (2013) The small GTPase Rif is dispensable for platelet filopodia generation in mice.
    PLoS One. 8 (1): e54663.
  16. Williams CM et al. (2015) Identification of roles for the SNARE-associated protein, SNAP29, in mouse platelets.
    Nov 20. [Epub ahead of print]
  17. Cuccurullo, A. et al. (2016) Blockade of Thrombopoietin Reduces Organ Damage in Experimental Endotoxemia and Polymicrobial Sepsis.
    PLoS One. 11 (3): e0151088.
  18. Criel, M. et al. (2016) Absence of Pear1 does not affect murine platelet function in vivo.
    Thromb Res. 146: 76-83.
  19. Ryan, J. et al. (2016) Myeloid cell-mediated renal injury in rapidly progressive glomerulonephritis depends upon spleen tyrosine kinase.
    J Pathol. 238 (1): 10-20.
  20. Woods SJ et al. (2015) Kinetic profiling of in vivo lung cellular inflammatory responses to mechanical ventilation.
    Am J Physiol Lung Cell Mol Physiol. 308 (9): L912-21.
  21. Asai, J. et al. (2016) Platelets Regulate the Migration of Keratinocytes via Podoplanin/CLEC-2 Signaling during Cutaneous Wound Healing in Mice.
    Am J Pathol. 186 (1): 101-8.
  22. Thomson, A.K. et al. (2017) Survival of motor neurone protein is required for normal postnatal development of the spleen.
    J Anat. 230 (2): 337-46.