CD4 antibody | YTS191.1
Rat anti Mouse CD4 antibody, clone YTS191.1 (MCA1767GA) used for the detection of CD4 positive lymphocytes in mouse liver metastases by immunohistochemistry and immunofluorescence on acetone trated cryosections.
Image caption:
(a) Immunohistochemistry and (b) immunofluorescence double staining: Representative image of CD4 (red)/CD8 (blue) (top) expression and Foxp3 (FITC)/CD4 (Cy3) (bottom) expression in liver metastases on day 10 (n = 7) compared to increased CD4 (red)/CD8 (blue) expression and Foxp3 (FITC)/CD4 (Cy3) expression on day 20. Immunohistochemistry: Nova red brick (red color), Vector Blue (blue color). Heamalum blue color-nuclear counterstaining. Asterisk with indication lines show metastases next to normal tissue (×100 and ×400). Immunofluorescence: FITC green fluorescein isothiocyanate, Cy3 red and DAPI 4',6-diamidino-2-phenylindole blue - nuclear counterstaining (×250).
From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A.
Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.>BMC Cancer. 2010 Mar 7;10:82.
Rat anti Mouse CD4 antibody, clone YTS191.1 (MCA1767) used to identify CD4 T helper lymphocytes in murine optic nerve tissue by immunofluorescence.
Image caption:
Therapeutic fingolimod and teriflunomide treatment attenuates neuroinflammation in CLN1 mice.
(A) Representative immune fluorescence microscopy and quantification of CD8+ and (B) CD4+ T-lymphocytes in longitudinal optic nerve sections from 7-month-old Ppt1+/+ , Ppt1-/-, fingolimod-treated Ppt1-/- (+ FTY720) and teriflunomide-treated Ppt1-/- (+ Teriflunomide) mice (circle = mean value of one mouse; n = 5 mice per group, One-way ANOVA and Tukey's post hoc test). Scale bars: 20 μm.
From: Janos Groh, Kristina Berve, Rudolf Martini,
Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset,
Brain Communications, 2021;, fcab047,
doi: 10.1093/braincomms/fcab047
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD4 antibody, clone YTS191.1 (MCA1767) used to identify CD4 expressing cells in mouse brain by immunohistochemistry on cryosections.
Image caption:
Adoptive transfer of Treg diminishes neuroinflammation in the nigrostriatal tract of hαSyn PD mice; CD28SA decreases early T cell activation in brain and modulates early IL-2 response in peripheral lymphatic organs of hαSyn PD mice; delayed treatment with CD28SA fails to reduce SN dopaminergic neurodegeneration in hαSyn PD mice. A-D Immunohistochemical stainings and quantification for CD4+ (A, B) and CD8+ (C, D) T cells in the SN and striatum of hαSyn-injected mice with either PBS or Treg delivery. Statistical analysis by unpaired two-tailed t-test: SN CD4+: t(14) = 2.367, P = 0.0329; striatum CD4+: t(14) = 2.601, P = 0.0209; SN CD8+: t(14) = 2.835, P = 0.0132; striatum CD8+: t(14) = 5.2, P = 0.0001. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars: 20 μm each.
From: Badr M, McFleder RL, Wu J, Knorr S, Koprich JB, Hünig T, Brotchie JM, Volkmann J, Lutz MB, Ip CW.
Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice.
J Neuroinflammation. 2022 Dec 31;19(1):319.
doi: 10.1186/s12974-022-02685-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD4 antibody, clone YTS191.1 (MCA1767) used to identify CD4 expressing cells in mouse brain by immunohistochemistry on cryosections.
Image caption:
CD28SA reduces neuroinflammation in the nigrostriatal tract of hαSyn PD mice. A-F Immunohistochemical stainings and quantification for CD4+ (A–C). Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: SN CD4+, F(3, 36) = 10.16, P < 0.0001; striatum CD4+, F(3, 21) = 4.263, P = 0.0168.
From: Badr M, McFleder RL, Wu J, Knorr S, Koprich JB, Hünig T, Brotchie JM, Volkmann J, Lutz MB, Ip CW.
Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice.
J Neuroinflammation. 2022 Dec 31;19(1):319.
doi: 10.1186/s12974-022-02685-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD4 antibody, clone YTS191.1 (MCA1767) used to detect CD4 expressing cells in murine spinal cord sections by immunofluorescence.
Image caption:
Disruption of the blood spinal cord barrier (BSCB) by low intensity pulsed ultrasound (US) enhances CNS lymphocyte infiltration in ALS mice. (a–l) Images of spinal cord sections of symptomatic stage ALS mice in the four different groups, immunostained for the lymphocyte pan-T CD3 (a,d,g,j, green) and specific lymphocyte T CD4 (b,e,h,k, red) markers and merged (c,f,i,l). Scale bars: 50 μm in (a) for (a–l) and 20 μm in inset of (a) for insets in (a–l). (m,n) Number of lymphocytes in the lumbar spinal cords of the different groups at the symptomatic stage (left graphs) or at disease end stage (right graphs), measured by counting the total number of CD3+ lymphocytes (m) or CD4+ cells (n) per spinal cord section (symptomatic, control n = 3, US n = 4, IGF1 n = 4, US IGF1 n = 5, end stage, control n = 6, US n = 5, IGF1 n = 4, US IGF1 n = 5). Bars represent mean with 95% CI. p values were determined by ANOVA followed by a post hoc Šídák multiple comparison test. (o–q) Images of a spinal cord section of a symptomatic stage ALS mouse that underwent US treatment, immunostained for the lymphocyte pan-T CD3 (o,q, green) and the cell proliferation marker Ki67 (p,q, red). Note that lymphocytes (arrowheads) are not Ki67 positive (arrows). Scale bar: 50 μm in (o) for (o–q).
From: Montero AS, Aliouat I, Ribon M, Canney M, Goldwirt L, Mourah S, Berriat F, Lobsiger CS, Pradat PF, Salachas F, Bruneteau G, Carpentier A, Boillée S.
Effect of ultrasound-mediated blood-spinal cord barrier opening on survival and motor function in females in an amyotrophic lateral sclerosis mouse model.
EBioMedicine. 2024 Jul 11;106:105235.
doi: 10.1016/j.ebiom.2024.105235.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD4
- Product Type
- Monoclonal Antibody
- Clone
- YTS191.1
- Isotype
- IgG2b
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD4 antibody, clone YTS191.1 recognizes the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes. Rat anti Mouse CD4 antibody, clone YTS191.1 exhibits depleting activity when used in vivo ( |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- MCA1767GA: IgG concentration 1 mg/ml
- MCA1767T, MCA1767: IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 Pack Size: 0.1 mg 1/100 Pack Size: 25 µg, 0.25 mg |
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD4 antibody
-
Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
Eur J Immunol. 20 (12): 2747-55. -
Bemelman, F. et al. (1998) Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose.
J Immunol. 160 (6): 2645-8. -
Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
J Immunol. 162 (1): 486-93. -
Croxford, J.L. et al. (2001) Different therapeutic outcomes in experimental allergic encephalomyelitis dependent upon the mode of delivery of IL-10: a comparison of the effects of protein, adenoviral or retroviral IL-10 delivery into the central nervous system.
J Immunol. 166: 4124-30. -
Eller, K. et al. (2011) IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression.
J Immunol. 186: 83-91. -
Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
Am J Pathol. 173: 119-29. -
Grimm, M.et al. (2010) Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
BMC Cancer. 10: 82. -
Jégou, J.F. et al. (2007) C3d Binding to the Myelin Oligodendrocyte Glycoprotein Results in an Exacerbated Experimental Autoimmune Encephalomyelitis
J Immunol. 178: 3323-31.
View The Latest Product References
-
Huber, J.M. et al. (2009) The proteasome inhibitor bortezomib aggravates renal ischemia-reperfusion injury.
Am J Physiol Renal Physiol. 297: F451-60. -
Wolf, D. et al. (2005) CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice.
J Am Soc Nephrol. 16: 1360-70. -
Abdulreda, M.H. et al. (2011) High-resolution, noninvasive longitudinal live imaging of immune responses.
Proc Natl Acad Sci U S A. 108: 12863-8. -
Nakashima, H. et al. (2011) A Novel Combination Immunotherapy for Cancer by IL-13Rα2-Targeted DNA Vaccine and Immunotoxin in Murine Tumor Models.
J Immunol. 187: 4935-46. -
Scotland, R.S. et al. (2011) Sex-differences in resident immune cell phenotype underlies more efficient acute inflammatory responses in female mice.
Blood. 118: 5918-27. -
Zitt, E. et al. (2011) The selective mineralocorticoid receptor antagonist eplerenone is protective in mild anti-GBM glomeru-lonephritis.
Int J Clin Exp Pathol. 4:606-15. -
Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
Sci Rep. 10 (1): 15157. -
Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
Brain Communications. fcab047. -
Karikari, A.A. et al. (2022) Neurodegeneration by α-synuclein-specific T cells in AAV-A53T-α-synuclein Parkinson's disease mice.
Brain Behav Immun. 101: 194-210. -
Badr, M. et al. (2022) Expansion of regulatory T cells by CD28 superagonistic antibodies attenuates neurodegeneration in A53T-α-synuclein Parkinson's disease mice.
J Neuroinflammation. 19 (1): 319. -
McFleder, R.L. et al. (2023) Brain-to-gut trafficking of alpha-synuclein by CD11c(+) cells in a mouse model of Parkinson's disease.
Nat Commun. 14 (1): 7529. -
Griffiths, M.R. et al. (2018) CD93 regulates central nervous system inflammation in two mouse models of autoimmune encephalomyelitis.
Immunology. 155 (3): 346-55. -
Mahadevan, K.K. et al. (2024) Type I conventional dendritic cells facilitate immunotherapy in pancreatic cancer.
Science. 384 (6703): eadh4567. -
Aringer, I. et al. (2021) Agonism of Prostaglandin E2 Receptor 4 Ameliorates Tubulointerstitial Injury in Nephrotoxic Serum Nephritis in Mice.
J Clin Med. 10 (4): 832. -
Rauschenberger, L. et al. (2022) Age-dependent neurodegeneration and neuroinflammation in a genetic A30P/A53T double-mutated α-synuclein mouse model of Parkinson's disease.
Neurobiol Dis. 171: 105798. -
Karikari, A.A. et al. (2022) Neurodegeneration by α-synuclein-specific T cells in AAV-A53T-α-synuclein Parkinson's disease mice.
Brain Behav Immun. 101: 194-210. -
Montero, A.S. et al. (2024) Effect of ultrasound-mediated blood-spinal cord barrier opening on survival and motor function in females in an amyotrophic lateral sclerosis mouse model.
EBioMedicine. 106: 105235.
- Synonyms
- L3T4 Antigen
- LY-4
- RRID
- AB_322769
- UniProt
- P06332
- Entrez Gene
- Cd4
- GO Terms
- GO:0001816 cytokine production
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0045058 T cell selection
- View More GO Terms
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050870 positive regulation of T cell activation
- GO:0051789 response to protein stimulus
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