CD4 antibody | YTS191.1

Rat anti Mouse CD4

Product Type
Monoclonal Antibody
Clone
YTS191.1
Isotype
IgG2b
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA1767GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.1 mg loader
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loader
MCA1767T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 25 µg loader
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loader
MCA1767
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD4 antibody, clone YTS191.1 recognizes the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes.

Rat anti Mouse CD4 antibody, clone YTS191.1 exhibits depleting activity when used in vivo (Bemelman et al. 1998).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Pack Size: 25 µg, 0.25 mg
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200 Pack Size: 0.1 mg
1/100 Pack Size: 25 µg, 0.25 mg
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD4 antibody

  1. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
    Eur J Immunol. 20 (12): 2747-55.
  2. Bemelman, F. et al. (1998) Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose.
    J Immunol. 160 (6): 2645-8.
  3. Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
    J Immunol. 162 (1): 486-93.
  4. Croxford, J.L. et al. (2001) Different therapeutic outcomes in experimental allergic encephalomyelitis dependent upon the mode of delivery of IL-10: a comparison of the effects of protein, adenoviral or retroviral IL-10 delivery into the central nervous system.
    J Immunol. 166: 4124-30.
  5. Eller, K. et al. (2011) IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression.
    J Immunol. 186: 83-91.
  6. Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
    Am J Pathol. 173: 119-29.
  7. Grimm, M.et al. (2010) Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
    BMC Cancer. 10: 82.
  8. Jégou, J.F. et al. (2007) C3d Binding to the Myelin Oligodendrocyte Glycoprotein Results in an Exacerbated Experimental Autoimmune Encephalomyelitis
    J Immunol. 178: 3323-31.
  9. View The Latest Product References
  10. Huber, J.M. et al. (2009) The proteasome inhibitor bortezomib aggravates renal ischemia-reperfusion injury.
    Am J Physiol Renal Physiol. 297: F451-60.
  11. Wolf, D. et al. (2005) CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice.
    J Am Soc Nephrol. 16: 1360-70.
  12. Abdulreda, M.H. et al. (2011) High-resolution, noninvasive longitudinal live imaging of immune responses.
    Proc Natl Acad Sci U S A. 108: 12863-8.
  13. Nakashima, H. et al. (2011) A Novel Combination Immunotherapy for Cancer by IL-13Rα2-Targeted DNA Vaccine and Immunotoxin in Murine Tumor Models.
    J Immunol. 187: 4935-46.
  14. Scotland, R.S. et al. (2011) Sex-differences in resident immune cell phenotype underlies more efficient acute inflammatory responses in female mice.
    Blood. 118: 5918-27.
  15. Zitt, E. et al. (2011) The selective mineralocorticoid receptor antagonist eplerenone is protective in mild anti-GBM glomeru-lonephritis.
    Int J Clin Exp Pathol. 4:606-15.
  16. Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
    Sci Rep. 10 (1): 15157.
  17. Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
    Brain Communications. fcab047.
  18. Karikari, A.A. et al. (2022) Neurodegeneration by α-synuclein-specific T cells in AAV-A53T-α-synuclein Parkinson's disease mice.
    Brain Behav Immun. 101: 194-210.

Flow Cytometry

Immunohistology - Frozen

Synonyms
L3T4 Antigen
LY-4
RRID
AB_322769
UniProt
P06332
Entrez Gene
Cd4
GO Terms
GO:0001816 cytokine production
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0006955 immune response
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0045058 T cell selection
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050870 positive regulation of T cell activation
GO:0051789 response to protein stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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