CD4 antibody | YTS177.9
Rat anti Mouse CD4:FITC
Rat anti Mouse CD4:Low Endotoxin
Rat anti Mouse CD4:RPE
- Product Type
- Monoclonal Antibody
- Clone
- YTS177.9
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1107F | F | 0.1 mg |
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MCA1107EL | F FN | 0.5 mg |
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MCA1107PE | F | 100 Tests |
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Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P06332
- Entrez Gene
- Cd4
- GO Terms
- GO:0001816 cytokine production
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0045058 T cell selection
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050870 positive regulation of T cell activation
- GO:0051789 response to protein stimulus
- Regulatory
- For research purposes only
Applications of CD4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/50 | 1/200 | |
Functional Assays | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
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Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | F | 0.5 mg |
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Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
References for CD4 antibody
-
Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
Eur J Immunol. 20 (12): 2737-45. -
Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
Eur J Immunol. 20 (12): 2747-55. -
Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
J Immunol. 161 (11): 5813-6. -
Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
Blood. 119 (9): 2056-65. -
Agua-Doce, A. and Graca, L. (2011) Prevention of house dust mite induced allergic airways disease in mice through immune tolerance.
PLoS One. 6: e22320. -
Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
J Immunol. 179: 3648-54. -
Saunders, A. et al. (2009) Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria.
Malar J. 8: 53. -
Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
Blood. 119: 2056-65. -
Kim, I. et al. (2017) Ibrutinib suppresses alloantibody responses in a mouse model of allosensitization.
Transpl Immunol. 45: 59-64. -
Agnieszka, S. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model
Journal of Immunology Research. 2019: 1-19.
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