CD4 antibody | YTS177.9

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Rat anti Mouse CD4:FITC

Rat anti Mouse CD4:Low Endotoxin

Rat anti Mouse CD4:RPE

Product Type
Monoclonal Antibody
Clone
YTS177.9
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1107F F 0.1 mg
MCA1107EL F FN 0.5 mg
MCA1107PE F 100 Tests
Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with the Mouse CD4 antigen, non polymorphic epitope (Qin et al. 1990). Rat anti Mouse CD4 antibody, Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with CD4 transfectants (Cobbold et al. 1990).Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance (Qin et al. 1990) and Cobbold et al. 1990).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Mouse spleen cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P06332 Related reagents
Entrez Gene
Cd4 Related reagents
GO Terms
GO:0001816 cytokine production
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0006955 immune response
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0045058 T cell selection
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050870 positive regulation of T cell activation
GO:0051789 response to protein stimulus
Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/200
Functional Assays
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL 0.5 mg F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for CD4 antibody

  1. Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
    Eur J Immunol. 20 (12): 2737-45.
  2. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
    Eur J Immunol. 20 (12): 2747-55.
  3. Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
    J Immunol. 161 (11): 5813-6.
  4. Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
    Blood. 119 (9): 2056-65.
  5. Agua-Doce, A. and Graca, L. (2011) Prevention of house dust mite induced allergic airways disease in mice through immune tolerance.
    PLoS One. 6: e22320.
  6. Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
    J Immunol. 179: 3648-54.
  7. Saunders, A. et al. (2009) Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria.
    Malar J. 8: 53.
  8. Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
    Blood. 119: 2056-65.
  9. Kim, I. et al. (2017) Ibrutinib suppresses alloantibody responses in a mouse model of allosensitization.
    Transpl Immunol. 45: 59-64.