CD4 antibody | YTS177.9

Rat anti Mouse CD4:FITC

Product Type
Monoclonal Antibody
Clone
YTS177.9
Isotype
IgG2a
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA1107F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
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Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with the Mouse CD4 antigen, non polymorphic epitope (Qin et al. 1990). Rat anti Mouse CD4 antibody, Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with CD4 transfectants (Cobbold et al. 1990).Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance (Qin et al. 1990) and Cobbold et al. 1990).

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Mouse spleen cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
List Price Your Price
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Description Rat IgG2a Negative Control:FITC

References for CD4 antibody

  1. Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
    Eur J Immunol. 20 (12): 2737-45.
  2. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
    Eur J Immunol. 20 (12): 2747-55.
  3. Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
    J Immunol. 161 (11): 5813-6.
  4. Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
    Blood. 119 (9): 2056-65.
  5. Agua-Doce, A. and Graca, L. (2011) Prevention of house dust mite induced allergic airways disease in mice through immune tolerance.
    PLoS One. 6: e22320.
  6. Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
    J Immunol. 179: 3648-54.
  7. Saunders, A. et al. (2009) Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria.
    Malar J. 8: 53.
  8. Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
    Blood. 119: 2056-65.
  9. View The Latest Product References
  10. Kim, I. et al. (2017) Ibrutinib suppresses alloantibody responses in a mouse model of allosensitization.
    Transpl Immunol. 45: 59-64.
  11. Agnieszka, S. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model
    Journal of Immunology Research. 2019: 1-19.

Flow Cytometry

Functional Assays

Synonyms
L3T4 Antigen
LY-4
RRID
AB_321372
UniProt
P06332
Entrez Gene
Cd4
GO Terms
GO:0001816 cytokine production
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0006955 immune response
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0045058 T cell selection
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050870 positive regulation of T cell activation
GO:0051789 response to protein stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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