CD4 antibody | YTS177.9

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Flow Cytometry

Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Rat IgG2a Negative Control:FITC	MCA1212F	F	100 Tests		Log in	- +	Add to Cart Add to Quote Get Quote
List Price	Your Price
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Description	Rat IgG2a Negative Control:FITC

References for CD4 antibody

1. Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
   Eur J Immunol. 20 (12): 2737-45.
2. Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
   Eur J Immunol. 20 (12): 2747-55.
3. Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
   J Immunol. 161 (11): 5813-6.
4. Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
   Blood. 119 (9): 2056-65.
5. Agua-Doce, A. and Graca, L. (2011) Prevention of house dust mite induced allergic airways disease in mice through immune tolerance.
   PLoS One. 6: e22320.
6. Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
   J Immunol. 179: 3648-54.
7. Saunders, A. et al. (2009) Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria.
   Malar J. 8: 53.
8. Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
   Blood. 119: 2056-65.
View The Latest Product References
9. Kim, I. et al. (2017) Ibrutinib suppresses alloantibody responses in a mouse model of allosensitization.
   Transpl Immunol. 45: 59-64.
10. Agnieszka, S. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model
    Journal of Immunology Research. 2019: 1-19.

Flow Cytometry

Functional Assays

Synonyms

L3T4 Antigen

LY-4

RRID

AB_321372

UniProt

P06332

Entrez Gene

Cd4

GO Terms

GO:0001816 cytokine production

GO:0007155 cell adhesion

GO:0016021 integral to membrane

GO:0005788 endoplasmic reticulum lumen

GO:0005789 endoplasmic reticulum membrane

GO:0006955 immune response

GO:0007166 cell surface receptor linked signaling pathway

GO:0009897 external side of plasma membrane

GO:0045058 T cell selection

View More GO Terms

GO:0045121 membrane raft

GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation

GO:0050850 positive regulation of calcium-mediated signaling

GO:0050870 positive regulation of T cell activation

GO:0051789 response to protein stimulus