CD301 antibody | ER-MP23








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IF F P ResetRat anti Mouse CD301
- Product Type
- Monoclonal Antibody
- Clone
- ER-MP23
- Isotype
- IgG2a
- Specificity
- CD301
Rat anti Mouse CD301 antibody, clone ER-MP23 recognizes murine CD301, a ~38 kDa cell surface protein, otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR). In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated. Rat anti Mouse CD301 antibody, clone ER-MP23 is reported to block the function of mouse CD301 (Dupasquier et al. 2006). Rat anti Mouse CD301 antibody, clone ER-MP23 binds both MGL1 and MGL2 homologues. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Balb/c macrophage precursor cell hybrids.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/100 | 1/200 |
Immunofluorescence | ![]() |
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Immunohistology - Frozen | ![]() |
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Immunohistology - Paraffin 1 | ![]() |
- 1Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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List Price | Your Price | ||||||
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Description | Rat IgG2a Negative Control |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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Description | Antigen Retrieval Buffer, pH8.0 |
References for CD301 antibody
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Leenen, P.J et al. (1994) Markers of mouse macrophage development detected by monoclonal antibodies.
J Immunol Methods. 174 (1-2): 5-19. -
Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
J Leukoc Biol. 78 (4): 845-52. -
Abadie, V. et al. (2005) Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes.
Blood. 106: 1843-50. -
Dupasquier, M. et al. (2004) Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis.
J Invest Dermatol. 123: 876-9. -
Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
J Clin Invest. 121: 985-97. -
Westcott, D.J. et al. (2009) MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity.
J Exp Med. 206: 3143-56. -
Fischer-Posovszky, P. et al. (2011) Targeted deletion of adipocytes by apoptosis leads to adipose tissue recruitment of alternatively activated m2 macrophages.
Endocrinology. 152: 3074-81. -
Spite, M. et al. (2011) Deficiency of the Leukotriene B4 Receptor, BLT-1, Protects against Systemic Insulin Resistance in Diet-Induced Obesity.
J Immunol. 187: 1942-9.
View The Latest Product References
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Raes, G. et al. (2005) Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation.
J Leukoc Biol. 77: 321-7. -
Freire, T. et al. (2010) Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.
Blood. 116: 3526-36. -
Blyszczuk, P. et al. (2013) Nitric oxide synthase 2 is required for conversion of pro-fibrogenic inflammatory CD133(+) progenitors into F4/80(+) macrophages in experimental autoimmune myocarditis.
Cardiovasc Res. 97 (2): 219-29. -
Ferret-Bernard, S. et al. (2012) Plasma membrane proteomes of differentially matured dendritic cells identified by LC-MS/MS combined with iTRAQ labelling.
J. Proteomics. 75: 938-48. -
Orr, J.S. et al. (2012) Toll-like Receptor 4 Deficiency Promotes the Alternative Activation of Adipose Tissue Macrophages.
Diabetes. 61: 2718-27. -
Wagner, M. et al. (2012) Inflamed tumor-associated adipose tissue is a depot for macrophages that stimulate tumor growth and angiogenesis.
Angiogenesis.15: 481-95 -
Dib, L.H. et al. (2014) Bone marrow leptin signaling mediates obesity-associated adipose tissue inflammation in male mice.
Endocrinology. 155: 40-6. -
Morris, M.E. et al. (2015) Systemically Delivered Adipose Stromal Vascular Fraction Cells Disseminate to Peripheral Artery Walls and Reduce Vasomotor Tone Through a CD11b+ Cell-Dependent Mechanism.
Stem Cells Transl Med. pii: sctm.2014-0252. -
Vukman KV et al. (2013) Mannose receptor and macrophage galactose-type lectin are involved in Bordetella pertussis mast cell interaction.
J Leukoc Biol. 94 (3): 439-48. -
Shah, R. et al. (2015) Metabolic Effects of CX3CR1 Deficiency in Diet-Induced Obese Mice.
PLoS One. 10 (9): e0138317. -
Hartwig, H. et al. (2015) Atherosclerotic Plaque Destabilization in Mice: A Comparative Study.
PLoS One. 10 (10): e0141019. -
Dupasquier, M. et al. (2006) The dermal microenvironment induces the expression of the alternative activation marker CD301/mMGL in mononuclear phagocytes, independent of IL-4/IL-13 signaling.
J Leukoc Biol. 80 (4): 838-49. -
Hanot Mambres, D. et al. (2015) In Situ Characterization of Splenic Brucella melitensis Reservoir Cells during the Chronic Phase of Infection in Susceptible Mice.
PLoS One. 10 (9): e0137835. -
Everts B et al. (2016) Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12.
J Exp Med. 213 (1): 35-51. -
Bartneck, M. et al. (2016) Histidine-rich glycoprotein promotes macrophage activation and inflammation in chronic liver disease.
Hepatology. 63 (4): 1310-24. -
Jha, A.K. et al. (2015) Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization.
Immunity. 42 (3): 419-30. -
Hellmann, J. et al. (2016) CCR7 Maintains Nonresolving Lymph Node and Adipose Inflammation in Obesity.
Diabetes. 65 (8): 2268-81. -
Manning, C.N. et al. (2015) Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro.
Stem Cell Res Ther. 6: 74. -
Braune, J. et al. (2017) IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity.
J Immunol. 198 (7): 2927-34. -
Zhang, H. et al. (2017) Synergistic Modulation of Inflammatory but not Metabolic Effects of High-Fat Feeding by CCR2 and CX3CR1.
Obesity (Silver Spring). 25 (8): 1410-20. -
Wagner, M. et al. (2019) Blockade of Lymphangiogenesis Shapes Tumor-Promoting Adipose Tissue Inflammation.
Am J Pathol. Jul 29 [Epub ahead of print]. -
Shimobayashi, M. et al. (2018) Insulin resistance causes inflammation in adipose tissue.
J Clin Invest. 128 (4): 1538-50. -
Baardman, J. et al. (2020) Macrophage ATP citrate lyase deficiency stabilizes atherosclerotic plaques.
Nat Commun. 11 (1): 6296. -
De La Cruz, A. et al. (2021) Platelet and Erythrocyte Extravasation across Inflamed Corneal Venules Depend on CD18, Neutrophils, and Mast Cell Degranulation.
Int J Mol Sci. 22 (14)Jul 08 [Epub ahead of print]. -
Lindhorst, A. et al. (2021) Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.
Cell Death Dis. 12 (6): 579. -
Huang, Y. et al. (2023) A pref-1-controlled non-inflammatory mechanism of insulin resistance.
iScience26,106923 -
Rigoni, T.S. et al. (2020) RANK Ligand Helps Immunity to Leishmania major by Skewing M2-Like Into M1 Macrophages.
Front Immunol. 11: 886. -
Xavier, M.N. et al. (2013) PPARγ-mediated increase in glucose availability sustains chronic Brucella abortus infection in alternatively activated macrophages.
Cell Host Microbe. 14 (2): 159-70. -
Hsu, S.N. et al. (2022) Increased PHOSPHO1 expression mediates cortical bone mineral density in renal osteodystrophy.
J Endocrinol. 254 (3): 153-67.
- Synonyms
- Macrophage Galactose Specific Lectin
- RRID
- AB_872014
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