CD301 antibody | ER-MP23

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MCA2392T
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C F IF P * 25 µg loader
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MCA2392
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C F IF P * 0.25 mg loader
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Rat anti Mouse CD301 antibody, clone ER-MP23 recognizes murine CD301, a ~38 kDa cell surface protein, otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR).

In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated.

Rat anti Mouse CD301 antibody, clone ER-MP23 is reported to block the function of mouse CD301 (Dupasquier et al. 2006). Rat anti Mouse CD301 antibody, clone ER-MP23 binds both MGL1 and MGL2 homologues.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Balb/c macrophage precursor cell hybrids.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
  1. 1Heat mediated antigen retrieval is required prior to staining paraffin-embedded sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD301 antibody

  1. Leenen, P.J et al. (1994) Markers of mouse macrophage development detected by monoclonal antibodies.
    J Immunol Methods. 174 (1-2): 5-19.
  2. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
    J Leukoc Biol. 78 (4): 845-52.
  3. Abadie, V. et al. (2005) Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes.
    Blood. 106: 1843-50.
  4. Dupasquier, M. et al. (2004) Macrophages and dendritic cells constitute a major subpopulation of cells in the mouse dermis.
    J Invest Dermatol. 123: 876-9.
  5. Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
    J Clin Invest. 121: 985-97.
  6. Westcott, D.J. et al. (2009) MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity.
    J Exp Med. 206: 3143-56.
  7. Fischer-Posovszky, P. et al. (2011) Targeted deletion of adipocytes by apoptosis leads to adipose tissue recruitment of alternatively activated m2 macrophages.
    Endocrinology. 152: 3074-81.
  8. Spite, M. et al. (2011) Deficiency of the Leukotriene B4 Receptor, BLT-1, Protects against Systemic Insulin Resistance in Diet-Induced Obesity.
    J Immunol. 187: 1942-9.
  9. View The Latest Product References
  10. Raes, G. et al. (2005) Macrophage galactose-type C-type lectins as novel markers for alternatively activated macrophages elicited by parasitic infections and allergic airway inflammation.
    J Leukoc Biol. 77: 321-7.
  11. Freire, T. et al. (2010) Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.
    Blood. 116: 3526-36.
  12. Lumeng, C.N. et al. (2008) Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes.
    Diabetes. 57: 3239-46.
  13. Blyszczuk, P. et al. (2013) Nitric oxide synthase 2 is required for conversion of pro-fibrogenic inflammatory CD133(+) progenitors into F4/80(+) macrophages in experimental autoimmune myocarditis.
    Cardiovasc Res. 97 (2): 219-29.
  14. Dib, L.H. et al. (2014) Bone marrow leptin signaling mediates obesity-associated adipose tissue inflammation in male mice.
    Endocrinology. 155: 40-6.
  15. Ferret-Bernard, S. et al. (2012) Plasma membrane proteomes of differentially matured dendritic cells identified by LC-MS/MS combined with iTRAQ labelling.
    J. Proteomics. 75: 938-48.
  16. Orr, J.S. et al. (2012) Toll-like Receptor 4 Deficiency Promotes the Alternative Activation of Adipose Tissue Macrophages.
    Diabetes. 61: 2718-27.
  17. Wagner, M. et al. (2012) Inflamed tumor-associated adipose tissue is a depot for macrophages that stimulate tumor growth and angiogenesis.
    Angiogenesis.15: 481-95
  18. Shah, R. et al. (2015) Metabolic Effects of CX3CR1 Deficiency in Diet-Induced Obese Mice.
    PLoS One. 10 (9): e0138317.
  19. Morris, M.E. et al. (2015) Systemically Delivered Adipose Stromal Vascular Fraction Cells Disseminate to Peripheral Artery Walls and Reduce Vasomotor Tone Through a CD11b+ Cell-Dependent Mechanism.
    Stem Cells Transl Med. pii: sctm.2014-0252.
  20. Vukman KV et al. (2013) Mannose receptor and macrophage galactose-type lectin are involved in Bordetella pertussis mast cell interaction.
    J Leukoc Biol. 94 (3): 439-48.
  21. Hartwig, H. et al. (2015) Atherosclerotic Plaque Destabilization in Mice: A Comparative Study.
    PLoS One. 10 (10): e0141019.
  22. Dupasquier, M. et al. (2006) The dermal microenvironment induces the expression of the alternative activation marker CD301/mMGL in mononuclear phagocytes, independent of IL-4/IL-13 signaling.
    J Leukoc Biol. 80 (4): 838-49.
  23. Hanot Mambres, D. et al. (2015) In Situ Characterization of Splenic Brucella melitensis Reservoir Cells during the Chronic Phase of Infection in Susceptible Mice.
    PLoS One. 10 (9): e0137835.
  24. Everts B et al. (2016) Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12.
    J Exp Med. 213 (1): 35-51.
  25. Bartneck, M. et al. (2016) Histidine-rich glycoprotein promotes macrophage activation and inflammation in chronic liver disease.
    Hepatology. 63 (4): 1310-24.
  26. Jha, A.K. et al. (2015) Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization.
    Immunity. 42 (3): 419-30.
  27. Hellmann, J. et al. (2016) CCR7 Maintains Nonresolving Lymph Node and Adipose Inflammation in Obesity.
    Diabetes. 65 (8): 2268-81.
  28. Manning, C.N. et al. (2015) Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro.
    Stem Cell Res Ther. 6: 74.
  29. Braune, J. et al. (2017) IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity.
    J Immunol. 198 (7): 2927-34.
  30. Zhang, H. et al. (2017) Synergistic Modulation of Inflammatory but not Metabolic Effects of High-Fat Feeding by CCR2 and CX3CR1.
    Obesity (Silver Spring). 25 (8): 1410-20.
  31. Wagner, M. et al. (2019) Blockade of Lymphangiogenesis Shapes Tumor-Promoting Adipose Tissue Inflammation.
    Am J Pathol. Jul 29 [Epub ahead of print].
  32. Shimobayashi, M. et al. (2018) Insulin resistance causes inflammation in adipose tissue.
    J Clin Invest. 128 (4): 1538-50.
  33. Baardman, J. et al. (2020) Macrophage ATP citrate lyase deficiency stabilizes atherosclerotic plaques.
    Nat Commun. 11 (1): 6296.
  34. De La Cruz, A. et al. (2021) Platelet and Erythrocyte Extravasation across Inflamed Corneal Venules Depend on CD18, Neutrophils, and Mast Cell Degranulation.
    Int J Mol Sci. 22 (14)Jul 08 [Epub ahead of print].
  35. Lindhorst, A. et al. (2021) Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.
    Cell Death Dis. 12 (6): 579.

Flow Cytometry

Immunohistology - Paraffin

Synonyms
Macrophage Galactose Specific Lectin
RRID
AB_872014
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