CD206 antibody | MR5D3
Rat anti Mouse CD206
- Product Type
- Monoclonal Antibody
- Clone
- MR5D3
- Isotype
- IgG2a
- Specificity
- CD206
Rat anti Mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells (Zamze et al. 2002). The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005). Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in western blotting (Martinez-Pomares et al. 2003 and Su et al. 2005). |
Our CD206 (MR5D3) Antibody has been referenced in >107 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Chimaeric CRD4-7-Fc protein
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Fischer rats were fused with cells of the Y3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | 1/10 | 1/20 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation |
- 1 CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. We recommend the use of Leucoperm (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cell in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control | MCA1212 | E F | 1 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Rat IgG2a Negative Control |
Source Reference
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Zamze, S. et al. (2002) Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor.
J Biol Chem. 277 (44): 41613-23.
References for CD206 antibody
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Martinez-Pomares, L. et al. (2003) Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies.
J Leukoc Biol. 73 (5): 604-13. -
Su, Y. et al. (2005) Glycosylation influences the lectin activities of the macrophage mannose receptor.
J Biol Chem. 280: 32811-20. -
Hassan, M.F. et al. (2006) The Schistosoma mansoni hepatic egg granuloma provides a favorable microenvironment for sustained growth of Leishmania donovani.
Am J Pathol. 169: 943-53. -
Devey, L. et al. (2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.
Mol Ther. 17: 65-72. -
Vetrone, S.A. et al. (2009) Osteopontin promotes fibrosis in dystrophic mouse muscle by modulating immune cell subsets and intramuscular TGF-beta.
J Clin Invest. 119: 1583-94. -
Autenrieth, S.E. & Autenrieth, I.B. (2009) Variable antigen uptake due to different expression of the macrophage mannose receptor by dendritic cells in various inbred mouse strains.
Immunology 127: 523-9. -
Westcott, D.J. et al. (2009) MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity.
J Exp Med. 206: 3143-56. -
Nair, M.G. et al. (2009) Alternatively activated macrophage-derived RELM-{alpha} is a negative regulator of type 2 inflammation in the lung.
J Exp Med. 206: 937-52.
View The Latest Product References
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Takagi, H. et al. (2009) Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages.
Glycobiology 19: 258-66. -
Bacci, M. et al. (2009) Macrophages are alternatively activated in patients with endometriosis and required for growth and vascularization of lesions in a mouse model of disease.
Am J Pathol. 175: 547-56. -
Hawkes, C.A. et al. (2009) Selective targeting of perivascular macrophages for clearance of beta-amyloid in cerebral amyloid angiopathy.
Proc Natl Acad Sci USA106: 1261-6. -
deSchoolmeester, M.L. et al. (2009) The mannose receptor binds Trichuris muris excretory/secretory proteins but is not essential for protective immunity.
Immunology 126: 246-55. -
Famulski, K.S. et al. (2010) Alternative macrophage activation-associated transcripts in T-cell-mediated rejection of mouse kidney allografts.
Am J Transplant 10 (3): 490-7. -
Hardison, S.E. et al. (2010) Pulmonary infection with an interferon-gamma-producing Cryptococcus neoformans strain results in classical macrophage activation and protection.
Am J Pathol. 176: 774-85. -
Lin, J.S. et al. (2010) Distinct roles of complement receptor 3, Dectin-1, and sialic acids in murine macrophage interaction with Histoplasma yeast.
J Leukoc Biol. 88: 95-106. -
Chavele, K.M. et al. (2010) Mannose receptor interacts with Fc receptors and is critical for the development of crescentic glomerulonephritis in mice.
J Clin Invest. 120: 1469-78. -
Hardison, S.E. et al. (2010) Interleukin-17 Is Not Required for Classical Macrophage Activation in a Pulmonary Mouse Model of Cryptococcus neoformans Infection.
Infect Immun. 78: 5341-51. -
Asano, J. et al. (2010) Nucleotide oligomerization binding domain-like receptor signaling enhances dendritic cell-mediated cross-priming in vivo.
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Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4 (5): e689. -
Zehner, M. et al. (2011) Mannose receptor polyubiquitination regulates endosomal recruitment of p97 and cytosolic antigen translocation for cross-presentation.
Proc Natl Acad Sci USA 108: 9933-8. -
Geier, H. & Celli, J. (2011) Phagocytic receptors dictate phagosomal escape and intracellular proliferation of Francisella tularensis.
Infect Immun. 79 (6): 2204-14. -
Deepe, G.S. Jr. & Buesing, W.R. (2011) Deciphering the Pathways of Death of Histoplasma capsulatum-Infected Macrophages: Implications for the Immunopathogenesis of Early Infection.
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Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy.
J Neurosci. 31: 3610-24. -
Sindrilaru, A. et al. (2011) An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
J Clin Invest. 121: 985-97. -
Schneider, D. et al. (2012) Neonatal rhinovirus infection induces mucous metaplasia and airways hyperresponsiveness.
J Immunol. 188 (6): 2894-904. -
Joyce, K.L. et al. (2012) Using eggs from Schistosoma mansoni as an in vivo model of helminth-induced lung inflammation.
J Vis Exp. Jun 5 (64): e3905. -
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Br J Cancer. 108 (6): 1288-97. -
Eskilsson, A. et al. (2014) Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.
J Comp Neurol. 522 (14): 3229-44. -
Espagnolle, N. et al. (2014) Specific Inhibition of the VEGFR-3 Tyrosine Kinase by SAR131675 Reduces Peripheral and Tumor Associated Immunosuppressive Myeloid Cells.
Cancers (Basel). 6 (1): 472-90. -
Sameshima, A. et al. (2015) Teneligliptin improves metabolic abnormalities in a mouse model of postmenopausal obesity.
J Endocrinol. 227 (1): 25-36. -
Manning, C.N. et al. (2015) Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro.
Stem Cell Res Ther. 6: 74. -
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Glia. 63 (9): 1606-20. -
O'Flaherty, B.M. et al. (2015) CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice.
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Øie, C.I. et al. (2016) FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides.
PLoS One. 11 (8): e0160602. -
Litvack ML et al. (2016) Alveolar-like Stem Cell-derived Myb(-) Macrophages Promote Recovery and Survival in Airway Disease.
Am J Respir Crit Care Med. 193 (11): 1219-29. -
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Hosono, K. et al. (2016) Signaling of Prostaglandin E Receptors, EP3 and EP4 Facilitates Wound Healing and Lymphangiogenesis with Enhanced Recruitment of M2 Macrophages in Mice.
PLoS One. 11 (10): e0162532. -
Rahman, K. et al. (2017) Inflammatory Ly6Chi monocytes and their conversion to M2 macrophages drive atherosclerosis regression.
J Clin Invest. 127 (8): 2904-2915. -
Brodaczewska, K. et al. (2017) Biodegradable Chitosan Decreases the Immune Response to Trichinella spiralis. in Mice.
Molecules. 22(11):2008. -
Braune, J. et al. (2017) IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity.
J Immunol. 198 (7): 2927-34. -
Bongiorno, E.K. et al. (2017) Type 1 Immune Mechanisms Driven by the Response to Infection with Attenuated Rabies Virus Result in Changes in the Immune Bias of the Tumor Microenvironment and Necrosis of Mouse GL261 Brain Tumors.
J Immunol. 198 (11): 4513-23. -
Orsini, F. et al. (2018) Mannose-Binding Lectin Drives Platelet Inflammatory Phenotype and Vascular Damage After Cerebral Ischemia in Mice via IL (Interleukin)-1α.
Arterioscler Thromb Vasc Biol. 38 (11): 2678-90. -
He, S. et al. (2018) Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis.
Scand J Immunol. 87 (4): e12648. -
Micanovic, R. et al. (2018) Tamm-Horsfall Protein Regulates Mononuclear Phagocytes in the Kidney.
J Am Soc Nephrol. 29 (3): 841-856. -
Igarashi, Y. et al. (2018) Partial depletion of CD206-positive M2-like macrophages induces proliferation of beige progenitors and enhances browning after cold stimulation.
Sci Rep. 8 (1): 14567. -
Han, Y.H. et al. (2019) A maresin 1/RORα/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis.
J Clin Invest. 130. pii: 124219 -
Cao, W. et al. (2019) Hoxa5 alleviates obesity-induced chronic inflammation by reducing ER stress and promoting M2 macrophage polarization in mouse adipose tissue.
J Cell Mol Med. 23 (10): 7029-42. -
Sui, A. et al. (2020) Inhibiting NF-κB Signaling Activation Reduces Retinal Neovascularization by Promoting a Polarization Shift in Macrophages.
Invest Ophthalmol Vis Sci. 61 (6): 4. -
Welc, S.S. et al. (2020) Modulation of Klotho expression in injured muscle perturbs Wnt signalling and influences the rate of muscle growth.
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Fan, A. et al. (2020) High-salt diet decreases mechanical thresholds in mice that is mediated by a CCR2-dependent mechanism.
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Shiau, D.J. et al. (2020) Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.
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Kishimoto, S. et al. (2020) Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model.
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Ackermann, J. et al. (2021) Myeloid Cell-Specific IL-4 Receptor Knockout Partially Protects from Adipose Tissue Inflammation.
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Flores, I. et al. (2021) Myeloid cell-mediated targeting of LIF to dystrophic muscle causes transient increases in muscle fiber lesions by disrupting the recruitment and dispersion of macrophages in muscle.
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Lindhorst, A. et al. (2021) Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.
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Catrysse, L. et al. (2021) A20 deficiency in myeloid cells protects mice from diet-induced obesity and insulin resistance due to increased fatty acid metabolism.
Cell Rep. 36 (12): 109748. -
Zhang, H. et al. (2021) Circulating Pro-Inflammatory Exosomes Worsen Stroke Outcomes in Aging.
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Wang, Y. et al. (2022) Myeloid cell-specific mutation of Spi1 selectively reduces M2-biased macrophage numbers in skeletal muscle, reduces age-related muscle fibrosis and prevents sarcopenia.
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Ba, H. et al. (2021) Suppression of Transmembrane Tumor Necrosis Factor Alpha Processing by a Specific Antibody Protects Against Colitis-Associated Cancer.
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- Synonyms
- Mannose Receptor C Type 1
- RRID
- AB_324622
- UniProt
- Q61830
- Entrez Gene
- Mrc1
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005537 mannose binding
- GO:0006897 endocytosis
- GO:0009986 cell surface
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