CD19 antibody | 6D5
Rat anti Mouse CD19 antibody, clone 6D5 (MCA1439) used to label B lymphocytes in mouse PBMCs by flow cytometry.
Image caption:
Flow cytometry representative protocol. The gate strategy was performed, as follow: (A) to exclude cell aggregates from analyses, cells were gated on Singlets region in FSC-A vs. FSC-H dot-plot; (B) a SSC-A vs. FSC-A dot plot was created from Singlets gate and Monos region was defined; from Monos gate, (C) CD4+/CD3+ T lymphocytes; (D) CD8+/CD3+ T lymphocytes, (E) CD19+ B lymphocytes and (F) F4/80+ macrophages were determined. (G) CD8+/CD4+ double-positive T lymphocytes were defined from CD3+ gate in CD3 vs. FSC-A dot plot (H). DN = Double Negatives.
From: Domingues CS, Cardoso FO, Hardoim DJ, Pelajo-Machado M, Bertho AL, Calabrese KDS.
Host Genetics Background Influence in the Intragastric Trypanosoma cruzi Infection.
Front Immunol. 2020 Nov 24;11:566476.
doi: 10.3389/fimmu.2020.566476.
This image is from an open access article distributed under terms of a Creative Commons Attribution License
Rat anti Mouse CD19:StarBright Violet 610
- Product Type
- Monoclonal Antibody
- Clone
- 6D5
- Isotype
- IgG2a
- Specificity
- CD19
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD19 antibody, clone 6D5 recognizes the murine CD19 cell surface antigen, a ~95 kDa glycoprotein expressed by B lymphocytes. Rat anti Mouse CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as clone Rat anti Mouse CD19 antibody, clone ID3 in cross-competition assays. StarBright Violet 670 conjugated Rat anti Mouse CD19 antibody, clone 6D5 (MCA1439SBV670) has been used successfully to label cells in an organ-on-chip platform by immunofluorescence (Cook et al. 2024 [preprint]). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to StarBright Violet 610 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Human K562 cell line transfected with murine CD19.
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 610 403 607 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Description | Mouse Seroblock FcR | ||||||
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| Description | Mouse Seroblock FcR | ||||||
References for CD19 antibody
-
Vernooy, J.H. et al. (2002) Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology.
Am J Respir Cell Mol Biol. 26 (1): 152-9. -
Andrew, D. and Aspinall. R. (2001) Il-7 and not stem cell factor reverses both the increase in apoptosis and the decline in thymopoiesis seen in aged mice.
J Immunol. 166: 1524-30. -
Bermudez-Fajardo, A. et al. (2011) The effect of Chlamydophila pneumoniae Major Outer Membrane Protein (MOMP) on macrophage and T cell-mediated immune responses.
Immunobiology. 216: 152-63. -
De Jesus, M. et al. (2009) Galactoxylomannan-mediated immunological paralysis results from specific B cell depletion in the context of widespread immune system damage.
J Immunol. 183: 3885-94. -
Jégou, J.F. et al. (2007) C3d binding to the myelin oligodendrocyte glycoprotein results in an exacerbated experimental autoimmune encephalomyelitis.
J Immunol. 178: 3323-31. -
Starck, J. et al. (2010) Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.
Blood. 116: 4795-805. -
Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
Blood. 118 (22): 5918-27. -
White, H.N. and Meng, Q.H. (2012) Recruitment of a Distinct but Related Set of VH Sequences into the Murine CD21hi/CD23- Marginal Zone B Cell Repertoire to That Seen in the Class-Switched Antibody Response.
J Immunol. 188: 287-93.
View The Latest Product References
-
Reynaud, J.M. et al. (2014) Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.
J Virol. 88: 5421-36. -
Candolfi, M. et al. (2011) B cells are critical to T-cell-mediated antitumor immunity induced by a combined immune-stimulatory/conditionally cytotoxic therapy for glioblastoma.
Neoplasia. 13: 947-60. -
Takabayashi, H. et al. (2014) Anti-inflammatory activity of bone morphogenetic protein signaling pathways in stomachs of mice.
Gastroenterology. 147: 396-406.e7. -
Weiss-Gayet, M. et al. (2016) Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.
PLoS One. 11 (4): e0153860. -
Meng, Q.H. & White, H.N. (2017) CD21int CD23+ follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses.
Immunology. 151 (2): 211-8. -
Mccubbrey, A.L. et al. (2016) MicroRNA-34a Negatively Regulates Efferocytosis by Tissue Macrophages in Part via SIRT1.
J Immunol. 196 (3): 1366-75. -
Vila-Caballer, M. et al. (2019) Disruption of the CCL1-CCR8 axis inhibits vascular Treg recruitment and function and promotes atherosclerosis in mice.
J Mol Cell Cardiol. 132: 154-63. -
Domingues, C.S. et al. (2020) Host Genetics Background Influence in the Intragastric Trypanosoma cruzi Infection.
Front Immunol. 11: 566476. -
Cook, S.R. et al. (2024) A 3D-printed multi-compartment organ-on-chip platform with a tubing-free pump models communication with the lymph node.
bioRχiv. Aug 04 [Epub ahead of print]. -
Lepland, A. et al. (2024) Therapeutic Tumor Macrophage Reprogramming in Breast Cancer Through a Peptide-Drug Conjugate
bioRχiv 12 Aug [Epub ahead of print].
- UniProt
- P25918
- Entrez Gene
- Cd19
- GO Terms
- GO:0016021 integral to membrane
- GO:0009897 external side of plasma membrane
- GO:0050853 B cell receptor signaling pathway
MCA1439SBV610
100004851
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