CD152 antibody | 1B8

Hamster anti Mouse CD152

Product Type
Monoclonal Antibody
Clone
1B8
Isotype
IgG
Specificity
CD152

Product Code Applications Pack Size List Price Your Price Qty
MCA4636GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF IP WB 0.1 mg loader
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loader
MCA4636
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF IP WB 0.25 mg loader
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loader
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Hamster anti Mouse CD152 antibody, clone 1B8 recognizes mouse CD152, commonly known as CTLA-4 (cytotoxic T-lymphocyte protein 4), a structural homolog for CD28. CD152 binds the same ligands as CD28, namely CD80 (B7.1) and CD86 (B7.2), but with much higher affinity. However, while binding of CD28 enhances T cell function, binding of CD152 inhibits it. CD152 is not detected on naïve T-cells and is induced upon T-cell activation.

In mice, lack of CD152 leads to massive lymphoproliferative disorder leading to tissue destruction and autoimmunity, suggesting a critical role for CD152 in limiting T-cell responses. CD152 has been shown to reduce IL-2 production after ligation and can inhibit T-cell expansion. This regulatory role and the high binding affinity of CD152 make it a potential therapy for autoimmune diseases such as rheumatoid arthritis.

Hamster anti Mouse CD152 antibody, clone 1B8 may be used to enhance proliferation and pro-inflammatory cytokines of lymph node cells. It may also be used to enhance tumour immunity.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Extracellular portion of mouse CD152 fused to a mouse IgG2a.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Lymph cells from immunised Armenian hamsters were fused with cells of the murine SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/100
Immunofluorescence
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
List Price Your Price
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Description Hamster (Armenian) IgG Negative Control

References for CD152 antibody

  1. Walunas, T. L. et al. (1994) CTLA-4 can function as a negative regulator of T cell activation.
    Immunity 1: 405-413.
  2. Verhagen, J. et al. (2013) CTLA-4 controls the thymic development of both conventional and regulatory T cells through modulation of the TCR repertoire.
    Proc Natl Acad Sci U S A. 110: E221-30.

Further Reading

  1. Karandikar, N.J. et al. (1996) CTLA-4: a negative regulator of autoimmune disease.
    J Exp Med. 184 (2): 783-8.

Immunofluorescence

Synonyms
CTLA-4
RRID
AB_10547302
UniProt
P09793
Entrez Gene
Ctla4
GO Terms
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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