CD121a antibody | JAMA-147
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Hamster anti Mouse CD121a
- Product Type
- Monoclonal Antibody
- Clone
- JAMA-147
- Isotype
- IgG
- Specificity
- CD121a
Antibody Quality and Performance Guarantee
| Hamster anti Mouse CD121a monoclonal antibody, clone JAMA-147 recognizes murine CD121a, the ~80 kDa type I interleukin-1 receptor. CD121a is a single pass type I membrane glycoprotein, weakly expressed on most cells and tissues including T lymphocytes, thymocytes, epithelial cells and dendritic cells, with stronger expression reported on fibroblasts. The ligands for CD121a are the interleukin-1 (IL-1) family members; IL-1 alpha; IL-1 beta and IL-1 receptor antagonist. IL-1 is a mediator of inflammation and can elicit a variety of other biological responses through binding to CD121a. Hamster anti mouse CD121 antibody, clone JAMA-147 has been reported to block IL-1 dependent cellular responses (Rogers et al. 1992). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Extracellular domain of murine IL-1R.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian Hamster were fused with cells of the mouse P3X63.Ag 8-653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| List Price | Your Price | ||||||
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| Description | Hamster (Armenian) IgG Negative Control | ||||||
Source Reference
-
Rogers, H.W. et al. (1992) Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice.
Proc Natl Acad Sci U S A. 89 (3): 1011-5.
References for CD121a antibody
-
Beckerman, K.P. et al. (1993) Release of nitric oxide during the T cell-independent pathway of macrophage activation. Its role in resistance to Listeria monocytogenes.
J Immunol. 150 (3): 888-95. -
Merrick, J.C. et al. (1997) Lymphocyte apoptosis during early phase of Listeria infection in mice.
Am J Pathol. 151 (3): 785-92. -
Scarim, A.L. et al. (1997) Evidence for the presence of type I IL-1 receptors on beta-cells of islets of Langerhans.
Biochim Biophys Acta. 1361 (3): 313-20. -
Kavanagh, D.P. et al. (2013) Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.
PLoS One. 8 (3): e59150.
- Synonyms
- Interleukin 1 Receptor Type 1
- RRID
- AB_566480
- UniProt
- P13504
- Entrez Gene
- Il1r1
- GO Terms
- GO:0016021 integral to membrane
- GO:0004909 interleukin-1, Type I, activating receptor activity
- GO:0009986 cell surface
- GO:0045087 innate immune response
MCA2352GA
151064
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