Hamster anti Mouse CD121a monoclonal antibody, clone JAMA-147
recognizes murine CD121a, the ~80 kDa type I interleukin-1 receptor. CD121a is a single pass type I membrane glycoprotein, weakly expressed on most cells and tissues including T lymphocytes, thymocytes, epithelial cells and dendritic cells, with stronger expression reported on fibroblasts.
The ligands for CD121a are the interleukin-1 (IL-1) family members; IL-1 alpha; IL-1 beta and IL-1 receptor antagonist. IL-1 is a mediator of inflammation and can elicit a variety of other biological responses through binding to CD121a.
Hamster anti mouse CD121 antibody, clone JAMA-147 has been reported to block IL-1 dependent cellular responses (Rogers et al. 1992
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Extracellular domain of murine IL-1R.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian Hamster were fused with cells of the mouse P3X63.Ag 8-653 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
interleukin-1, Type I, activating receptor activity
innate immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD121a antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
Rogers, H.W. et al. (1992) Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice.
Proc Natl Acad Sci U S A. 89 (3): 1011-5.
References for CD121a antibody
Scarim, A.L. et al. (1997) Evidence for the presence of type I IL-1 receptors on beta-cells of islets of Langerhans.
Biochim Biophys Acta. 1361 (3): 313-20.
Merrick, J.C. et al. (1997) Lymphocyte apoptosis during early phase of Listeria infection in mice.
Am J Pathol. 151 (3): 785-92.
Beckerman, K.P. et al. (1993) Release of nitric oxide during the T cell-independent pathway of macrophage activation. Its role in resistance to Listeria monocytogenes.
J Immunol. 150 (3): 888-95.
Kavanagh, D.P. et al. (2013) Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.
PLoS One. 8 (3): e59150.
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