CD121a antibody | JAMA-147

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Hamster anti Mouse CD121a:Alexa Fluor® 488

Hamster anti Mouse CD121a:Alexa Fluor® 647

Hamster anti Mouse CD121a

Product Type
Monoclonal Antibody
Clone
JAMA-147
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2352A488T F 25 Tests/0.25ml
MCA2352A488 F 100 Tests/1ml
MCA2352A647T F 25 Tests/0.25ml
MCA2352A647 F 100 Tests/1ml
MCA2352GA F IP 0.1 mg
Hamster anti Mouse CD121a monoclonal antibody, clone JAMA-147 recognizes murine CD121a, the ~80 kDa type I interleukin-1 receptor. CD121a is a single pass type I membrane glycoprotein, weakly expressed on most cells and tissues including T lymphocytes, thymocytes, epithelial cells and dendritic cells, with stronger expression reported on fibroblasts.

The ligands for CD121a are the interleukin-1 (IL-1) family members; IL-1 alpha; IL-1 beta and IL-1 receptor antagonist. IL-1 is a mediator of inflammation and can elicit a variety of other biological responses through binding to CD121a.

Hamster anti mouse CD121 antibody, clone JAMA-147 has been reported to block IL-1 dependent cellular responses (Rogers et al. 1992).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Extracellular domain of murine IL-1R.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Armenian Hamster were fused with cells of the mouse P3X63.Ag 8-653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P13504 Related reagents
Entrez Gene
Il1r1 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004909 interleukin-1, Type I, activating receptor activity
GO:0009986 cell surface
GO:0045087 innate immune response
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD121a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488 MCA2356A488 100 Tests/1ml F
Hamster (Armenian) IgG Negative Control:Alexa Fluor® 647 MCA2356A647 100 Tests/1ml F
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

Source Reference

  1. Rogers, H.W. et al. (1992) Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice.
    Proc Natl Acad Sci U S A. 89 (3): 1011-5.

References for CD121a antibody

  1. Wolyniec, K. et al. (2012) E6AP ubiquitin ligase regulates PML-induced senescence in Myc-driven lymphomagenesis
    Blood. 120: 822-32
  2. Scarim, A.L. et al. (1997) Evidence for the presence of type I IL-1 receptors on beta-cells of islets of Langerhans.
    Biochim Biophys Acta. 1361 (3): 313-20.
  3. Merrick, J.C. et al. (1997) Lymphocyte apoptosis during early phase of Listeria infection in mice.
    Am J Pathol. 151 (3): 785-92.
  4. Beckerman, K.P. et al. (1993) Release of nitric oxide during the T cell-independent pathway of macrophage activation. Its role in resistance to Listeria monocytogenes.
    J Immunol. 150 (3): 888-95.
  5. Kavanagh, D.P. et al. (2013) Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.
    PLoS One. 8 (3): e59150.