CD107b antibody | M3/84



The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Glycoproteins purified from mouse peritoneal macrophage membranes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P17047
- Entrez Gene
- Lamp2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0005770 late endosome
- GO:0010008 endosome membrane
- GO:0030670 phagocytic vesicle membrane
- Regulatory
- For research purposes only
Applications of CD107b antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | 1/50 | 1/100 | |
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
![]() |
|
Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
![]() |
|
Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
![]() |
|
Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
![]() |
|
Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
![]() |
|
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
![]() |
|
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
![]() |
|
Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
![]() |
|
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for CD107b antibody
-
Springer, T.A. (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.
J Biol Chem. 256 (8): 3833-9. -
Flotte, T.J. et al. (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
Am J Pathol. 111 (1): 112-24. -
Ho, M.K. & Springer, T.A. (1983) Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.
J Biol Chem. 258 (1): 636-42. -
Ulrich, R. et al. (2010) Machine learning approach identifies new pathways associated with demyelination in a viral model of multiple sclerosis.
J Cell Mol Med. 14 (1-2): 434-48. -
Amirbekian, V. et al. (2007) Detecting and assessing macrophages in vivo to evaluate atherosclerosis noninvasively using molecular MRI.
Proc Natl Acad Sci U S A. 104: 961-6. -
Fan, D. et al. (2014) Differential role of TIMP2 and TIMP3 in cardiac hypertrophy, fibrosis, and diastolic dysfunction.
Cardiovasc Res. 103 (2): 268-80. -
Higuchi, Y. et al. (2012) Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.
Am J Physiol Heart Circ Physiol. 302: H2352-62. -
Ishibashi, M. et al. (2004) Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling.
Circ Res. 94: 1203-10. -
Sato, A. et al. (2008) Thioredoxin-1 ameliorates cigarette smoke-induced lung inflammation and emphysema in mice.
J Pharmacol Exp Ther. 325: 380-8. -
Xu, J. et al. (2007) Role of cardiac overexpression of ANG II in the regulation of cardiac function and remodeling postmyocardial infarction.
Am J Physiol Heart Circ Physiol. 293: H1900-7. -
Zhao, Q. et al. (2004) Essential role of vascular endothelial growth factor in angiotensin II-induced vascular inflammation and remodeling.
Hypertension. 44: 264-70. -
Hansmann, F. et al. (2012) Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system.
Cell Transplant. 21 (6): 1161-75. -
Herder, V. et al. (2015) Dynamic Changes of Microglia/Macrophage M1 and M2 Polarization in Theiler's Murine Encephalomyelitis.
Brain Pathol. 25 (6): 712-23. -
Bröer S et al. (2016) Brain inflammation, neurodegeneration and seizure development following picornavirus infection markedly differ among virus and mouse strains and substrains.
Exp Neurol. pii: S0014-4886(16)30033-4. -
Bobbala, D. et al. (2016) Interleukin-21-dependent modulation of T cell antigen receptor reactivity towards low affinity peptide ligands in autoreactive CD8(+) T lymphocytes.
Cytokine. 85: 83-91. -
Raddatz, B.B. et al. (2016) Central Nervous System Demyelination and Remyelination is Independent from Systemic Cholesterol Level in Theiler's Murine Encephalomyelitis.
Brain Pathol. 26 (1): 102-19. -
Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
Brain Pathol. 28 (3): 349-368. -
Fayyad, A. et al. (2018) Matrix metalloproteinases expression in spontaneous canine histiocytic sarcomas and its xenograft model.
Vet Immunol Immunopathol. 198: 54-64. -
Li, L. et al. (2015) Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
J Neuroinflammation. 12: 242. -
Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
Vet Immunol Immunopathol. 202: 130-40.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra