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CD107b antibody | M3/84

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Anti Mouse CD107b Antibody, clone M3/84 thumbnail image 1 Anti Mouse CD107b Antibody, clone M3/84 thumbnail image 2 Anti Mouse CD107b Antibody, clone M3/84 thumbnail image 3
Anti Mouse CD107b Antibody, clone M3/84 gallery image 1
Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (MCA2293) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (MCA78G ) is included as a loading control. Detection is with Dylight®800 conjugated Goat anti Rat IgG: (STAR71D800GA)
Anti Mouse CD107b Antibody, clone M3/84 gallery image 2
Figure A. Alexa488® conjugated rat anti mouse CD68 (MCA1957A488) and Alexa647® conjugated Rat IgG2b isotype control. Figure B. Alexa488® conjugated rat anti mouse CD68 (MCA1957A488) and Alexa647® conjugated rat anti mouse CD107b (MCA2293A647). All experiments performed on murine peritonial macrophages in the presence of murine SeroBlock (BUF041A).
Anti Mouse CD107b Antibody, clone M3/84 gallery image 3
Published customer image:
Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293) used for the identification of MAC-3 expressing cells in mouse spinal cord sections by immunofluorescence.
Image caption:
ISG15 and PKR protein expression of astrocytes, oligodendrocytes, and microglia/macrophages in spinal cord white matter lesions at 98 dpi. ISG15 (green) is expressed by astrocytes (red, a), oligodendrocytes (red, c), and microglia/macrophages (red, e) demonstrated by an orange-yellow mixed color (arrows). No co-localization of PKR (green, arrowheads) with GFAP (red, arrows) indicates no/low PKR expression in astrocytes (red, b), whereas co-localization of PKR (green) with Olig2 (red, d) and MAC-3 (red, f) demonstrates PKR expression in oligodendrocytes and microglia (arrows). Immunofluorescence double-staining for ISG15 and PKR with GFAP, Olig2, and MAC-3. Nuclei are stained with bisbenzimide. Bar 25μm.

From: Li L, Ulrich R, Baumgärtner W, Gerhauser I.
Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
J Neuroinflammation. 2015 Dec 24;12:242.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Rat anti Mouse CD107b

Product Type
Monoclonal Antibody
Clone
M3/84
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2293GA
Datasheet
C F* IP P WB
MSDS
0.1 mg loader

MCA2293
Datasheet
C F* IP P WB
MSDS
0.25 mg loader

Rat anti Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. CD107b is involved in aspects of leucocyte adhesion (Kannan et al. 1996).

The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Glycoproteins purified from mouse peritoneal macrophage membranes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P17047
Entrez Gene
Lamp2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0005770 late endosome
GO:0010008 endosome membrane
GO:0030670 phagocytic vesicle membrane
Regulatory
For research purposes only

Applications of CD107b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for CD107b antibody

  1. Springer, T.A. (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.
    J Biol Chem. 256 (8): 3833-9.
  2. Flotte, T.J. et al. (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
    Am J Pathol. 111 (1): 112-24.
  3. Ho, M.K. & Springer, T.A. (1983) Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.
    J Biol Chem. 258 (1): 636-42.
  4. Ulrich, R. et al. (2010) Machine learning approach identifies new pathways associated with demyelination in a viral model of multiple sclerosis.
    J Cell Mol Med. 14 (1-2): 434-48.
  5. Amirbekian, V. et al. (2007) Detecting and assessing macrophages in vivo to evaluate atherosclerosis noninvasively using molecular MRI.
    Proc Natl Acad Sci U S A. 104: 961-6.
  6. Fan, D. et al. (2014) Differential role of TIMP2 and TIMP3 in cardiac hypertrophy, fibrosis, and diastolic dysfunction.
    Cardiovasc Res. 103 (2): 268-80.
  7. Higuchi, Y. et al. (2012) Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.
    Am J Physiol Heart Circ Physiol. 302: H2352-62.
  8. Ishibashi, M. et al. (2004) Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling.
    Circ Res. 94: 1203-10.
  9. Sato, A. et al. (2008) Thioredoxin-1 ameliorates cigarette smoke-induced lung inflammation and emphysema in mice.
    J Pharmacol Exp Ther. 325: 380-8.
  10. Xu, J. et al. (2007) Role of cardiac overexpression of ANG II in the regulation of cardiac function and remodeling postmyocardial infarction.
    Am J Physiol Heart Circ Physiol. 293: H1900-7.
  11. Zhao, Q. et al. (2004) Essential role of vascular endothelial growth factor in angiotensin II-induced vascular inflammation and remodeling.
    Hypertension. 44: 264-70.
  12. Hansmann, F. et al. (2012) Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system.
    Cell Transplant. 21 (6): 1161-75.
  13. Herder, V. et al. (2015) Dynamic Changes of Microglia/Macrophage M1 and M2 Polarization in Theiler's Murine Encephalomyelitis.
    Brain Pathol. 25 (6): 712-23.
  14. Bröer S et al. (2016) Brain inflammation, neurodegeneration and seizure development following picornavirus infection markedly differ among virus and mouse strains and substrains.
    Exp Neurol. pii: S0014-4886(16)30033-4.
  15. Bobbala, D. et al. (2016) Interleukin-21-dependent modulation of T cell antigen receptor reactivity towards low affinity peptide ligands in autoreactive CD8(+) T lymphocytes.
    Cytokine. 85: 83-91.
  16. Raddatz, B.B. et al. (2016) Central Nervous System Demyelination and Remyelination is Independent from Systemic Cholesterol Level in Theiler's Murine Encephalomyelitis.
    Brain Pathol. 26 (1): 102-19.
  17. Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
    Brain Pathol. 28 (3): 349-368.
  18. Fayyad, A. et al. (2018) Matrix metalloproteinases expression in spontaneous canine histiocytic sarcomas and its xenograft model.
    Vet Immunol Immunopathol. 198: 54-64.
  19. Li, L. et al. (2015) Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
    J Neuroinflammation. 12: 242.
  20. Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
    Vet Immunol Immunopathol. 202: 130-40.
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