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CD107b antibody | M3/84

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MCA2293GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IP P WB 0.1 mg loader
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loader
MCA2293
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SDS Safety Datasheet SDS
C F * IP P WB 0.25 mg loader
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loader

Rat anti Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. CD107b is involved in aspects of leucocyte adhesion (Kannan et al. 1996).

The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilization procedure prior to staining.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Glycoproteins purified from mouse peritoneal macrophage membranes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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Description Antigen Retrieval Buffer, pH8.0

References for CD107b antibody

  1. Springer, T.A. (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.
    J Biol Chem. 256 (8): 3833-9.
  2. Flotte, T.J. et al. (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
    Am J Pathol. 111 (1): 112-24.
  3. Ho, M.K. & Springer, T.A. (1983) Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.
    J Biol Chem. 258 (1): 636-42.
  4. Ulrich, R. et al. (2010) Machine learning approach identifies new pathways associated with demyelination in a viral model of multiple sclerosis.
    J Cell Mol Med. 14 (1-2): 434-48.
  5. Amirbekian, V. et al. (2007) Detecting and assessing macrophages in vivo to evaluate atherosclerosis noninvasively using molecular MRI.
    Proc Natl Acad Sci U S A. 104: 961-6.
  6. Fan, D. et al. (2014) Differential role of TIMP2 and TIMP3 in cardiac hypertrophy, fibrosis, and diastolic dysfunction.
    Cardiovasc Res. 103 (2): 268-80.
  7. Higuchi, Y. et al. (2012) Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.
    Am J Physiol Heart Circ Physiol. 302: H2352-62.
  8. Ishibashi, M. et al. (2004) Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling.
    Circ Res. 94: 1203-10.
  9. View The Latest Product References
  10. Sato, A. et al. (2008) Thioredoxin-1 ameliorates cigarette smoke-induced lung inflammation and emphysema in mice.
    J Pharmacol Exp Ther. 325: 380-8.
  11. Xu, J. et al. (2007) Role of cardiac overexpression of ANG II in the regulation of cardiac function and remodeling postmyocardial infarction.
    Am J Physiol Heart Circ Physiol. 293: H1900-7.
  12. Zhao, Q. et al. (2004) Essential role of vascular endothelial growth factor in angiotensin II-induced vascular inflammation and remodeling.
    Hypertension. 44: 264-70.
  13. Hansmann, F. et al. (2012) Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system.
    Cell Transplant. 21 (6): 1161-75.
  14. Herder, V. et al. (2015) Dynamic Changes of Microglia/Macrophage M1 and M2 Polarization in Theiler's Murine Encephalomyelitis.
    Brain Pathol. 25 (6): 712-23.
  15. Bröer S et al. (2016) Brain inflammation, neurodegeneration and seizure development following picornavirus infection markedly differ among virus and mouse strains and substrains.
    Exp Neurol. pii: S0014-4886(16)30033-4.
  16. Bobbala, D. et al. (2016) Interleukin-21-dependent modulation of T cell antigen receptor reactivity towards low affinity peptide ligands in autoreactive CD8(+) T lymphocytes.
    Cytokine. 85: 83-91.
  17. Raddatz, B.B. et al. (2016) Central Nervous System Demyelination and Remyelination is Independent from Systemic Cholesterol Level in Theiler's Murine Encephalomyelitis.
    Brain Pathol. 26 (1): 102-19.
  18. Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
    Brain Pathol. 28 (3): 349-368.
  19. Fayyad, A. et al. (2018) Matrix metalloproteinases expression in spontaneous canine histiocytic sarcomas and its xenograft model.
    Vet Immunol Immunopathol. 198: 54-64.
  20. Li, L. et al. (2015) Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
    J Neuroinflammation. 12: 242.
  21. Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
    Vet Immunol Immunopathol. 202: 130-40.
  22. Armando, F. et al. (2021) Intratumoral Canine Distemper Virus Infection Inhibits Tumor Growth by Modulation of the Tumor Microenvironment in a Murine Xenograft Model of Canine Histiocytic Sarcoma.
    Int J Mol Sci. 2 (7): 3578.
  23. Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
    Brain Pathol. 28 (3): 349-68.
  24. Waltl, I. et al. (2018) Microglia have a protective role in viral encephalitis-induced seizure development and hippocampal damage.
    Brain Behav Immun. 74: 186-204.
  25. Wiedemann, G.M. et al. (2016) A novel TLR7 agonist reverses NK cell anergy and cures RMA-S lymphoma-bearing mice.
    Oncoimmunology. 5 (7): e1189051.
  26. Dey, S. et al. (2021) Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis.
    JCI Insight. 6 (19): e149539.
  27. Iwaki, T. et al. (2021) Plasminogen Deficiency Significantly Reduces Vascular Wall Disease in a Murine Model of Type IIa Hypercholesterolemia
    Biomedicines. 9 (12): 1832.
  28. Bühler, M. et al. (2023) IFNAR signaling of neuroectodermal cells is essential for the survival of C57BL/6 mice infected with Theiler's murine encephalomyelitis virus.
    J Neuroinflammation. 20 (1): 58.
  29. Avloniti, M. et al. (2024) IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severity.
    J Neuroinflammation. 21 (1): 34.

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
MAC-3
RRID
AB_2249788
UniProt
P17047
Entrez Gene
Lamp2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0005770 late endosome
GO:0010008 endosome membrane
GO:0030670 phagocytic vesicle membrane

MCA2293GA

160239 1607

MCA2293

1701

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