CD107b antibody | M3/84
Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293) used for the identification of MAC-3 expressing cells in mouse spinal cord sections by immunofluorescence.
Image caption:
ISG15 and PKR protein expression of astrocytes, oligodendrocytes, and microglia/macrophages in spinal cord white matter lesions at 98 dpi. ISG15 (green) is expressed by astrocytes (red, a), oligodendrocytes (red, c), and microglia/macrophages (red, e) demonstrated by an orange-yellow mixed color (arrows). No co-localization of PKR (green, arrowheads) with GFAP (red, arrows) indicates no/low PKR expression in astrocytes (red, b), whereas co-localization of PKR (green) with Olig2 (red, d) and MAC-3 (red, f) demonstrates PKR expression in oligodendrocytes and microglia (arrows). Immunofluorescence double-staining for ISG15 and PKR with GFAP, Olig2, and MAC-3. Nuclei are stained with bisbenzimide. Bar 25μm.
From: Li L, Ulrich R, Baumgärtner W, Gerhauser I.
Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
J Neuroinflammation. 2015 Dec 24;12:242.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Biotinylated Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293B) used to demonstrate MAC-3 staining in murine pancreas by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Loss of miR-29a/b1 promotes enhanced and prolonged infiltration of immune cells in the pancreas of AP mice. Representative IHC staining of Mac3 in pancreatic tissue sections of saline (Cer-) and caerulein (Cer+) dosed WT and KO mice through the course of AP progression (Scale bars = 200μm).
From: Dey S, Udari LM, RiveraHernandez P, Kwon JJ, Willis B, Easler JJ, Fogel EL, Pandol S, Kota J.
Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis.
JCI Insight. 2021 Aug 31:149539.
doi: 10.1172/jci.insight.149539.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293) used to detect MAC3 expressing cells in mouse hippocampus by immunohistochemistry on paraffin embedded tissue sections.
Image caption:
GFAP+, MAC3+, and TMEM119+ cells in the hippocampus at 4 days after TMEV-BeAn infection. No significant differences in the semiquantitative analysis of GFAP+, MAC3+ and TMEM119+ cells were found between TMEV-infected IFNAR-/-, NesCre+/- IFNARfl/fl, GFAPCre+/- IFNARfl/fl, Syn1Cre+/- IFNARfl/fl and Cre-/- IFNARfl/fl mice (n=5-10). Shown are all data points with means. Each data point represents the semiquantitative score of immunopositive cells of one mouse.
From: IArmando F, Fayyad A, Arms S, Barthel Y, Schaudien D, Rohn K, Gambini M, Lombardo MS, Beineke A, Baumgärtner W, Puff C.
Intratumoral Canine Distemper Virus Infection Inhibits Tumor Growth by Modulation of the Tumor Microenvironment in a Murine Xenograft Model of Canine Histiocytic Sarcoma.
Int J Mol Sci. 2021 Mar 30;22 (7): 3578.
doi: 10.3390/ijms22073578.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293) used to demonstrate myeloid infiltration in mouse spinal cord by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Global depletion of myeloid IKKβ results in reduced axonal damage and oxidative stress in EAE. A Specimen images of paraffin-embedded coronal spinal cord sections from IKK&betaF/F mice with MOG35-55-induced EAE at 23 dpi (chronic phase). Serial sections were stained for markers of inflammation and demyelination, such as hematoxylin and eosin (H&E) and Luxol fast blue (LFB), respectively. Representative images from immunohistochemistry for markers of axonal damage (APP and Biel. Spheroids), immune cell infiltration (CD3 and Mac3), myeloid cell activation (iNOS) and macrophagic oxidative stress (p22 phox) are also shown.
From: Avloniti M, Evangelidou M, Gomini M, Loupis T, Emmanouil M, Mitropoulou A, Tselios T, Lassmann H, Gruart A, Delgado-García JM, Probert L, Kyrargyri V.
IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severity.
J Neuroinflammation. 2024 Jan 27;21(1):34.
doi: 10.1186/s12974-024-03023-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293) used to demonstrate myeloid infiltration in mouse spinal cord by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Global depletion of myeloid IKKβ results in reduced axonal damage and oxidative stress in EAE. A Specimen images of paraffin-embedded coronal spinal cord sections from ΜφΙΚΚβKO mice with MOG35-55-induced EAE at 23 dpi (chronic phase). Serial sections were stained for markers of inflammation and demyelination, such as hematoxylin and eosin (H&E) and Luxol fast blue (LFB), respectively. Representative images from immunohistochemistry for markers of axonal damage (APP and Biel. Spheroids), immune cell infiltration (CD3 and Mac3), myeloid cell activation (iNOS) and macrophagic oxidative stress (p22 phox) are also shown.
From: Avloniti M, Evangelidou M, Gomini M, Loupis T, Emmanouil M, Mitropoulou A, Tselios T, Lassmann H, Gruart A, Delgado-García JM, Probert L, Kyrargyri V.
IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severity.
J Neuroinflammation. 2024 Jan 27;21(1):34.
doi: 10.1186/s12974-024-03023-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Alexa Fluor 647 conjugated Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293A647) used to evaluate antigen expression on bone marrow derived mast cells by flow cytometry.
Image caption:
Mast cell activation is impaired when PDE3 is targeted. (A, B) Bone marrow-derived mast cells (MCs) obtained form Pde3−/− mice and wild-type (WT) control mice were sensitized with DNP-specific immunoglobulin E (IgE) or phosphate buffered saline (PBS), followed by stimulation with DNP-7 OVA. The mast cell activation markers CD107b and CD200r were evaluated and data are shown as histogram overlays. (C) Dot plots with median fluorescence intensity (MFI) values indicating reduction in CD107b and induction of CD200r upon DNP-7 OVA stimulation for WT and Pde3−/− mast cells. (D) ΔMFI for the indicated markers in MCs from WT and Pde3−/− mice (KO). A Wilcox signed test and a Mann-Whitney U test was used for statistical evaluation. *P <0.05. Plots represent (n=5) of one of two independent experiments. MFI, median fluorescence intensity.
From: Beute J, Ganesh K, Nastiti H, Hoogenboom R, Bos V, Folkerts J, Schreurs MWJ, Hockman S, Hendriks RW, KleinJan A.
PDE3 Inhibition Reduces Epithelial Mast Cell Numbers in Allergic Airway Inflammation and Attenuates Degranulation of Basophils and Mast Cells.
Front Pharmacol. 2020 May 1;11:470.
doi: 10.3389/fphar.2020.00470.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD107b
- Product Type
- Monoclonal Antibody
- Clone
- M3/84
- Isotype
- IgG1
- Specificity
- CD107b
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. CD107b is involved in aspects of leucocyte adhesion (Kannan et al. 1996). The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilization procedure prior to staining. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Glycoproteins purified from mouse peritoneal macrophage membranes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
References for CD107b antibody
-
Springer, T.A. (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.
J Biol Chem. 256 (8): 3833-9. -
Flotte, T.J. et al. (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
Am J Pathol. 111 (1): 112-24. -
Ho, M.K. & Springer, T.A. (1983) Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.
J Biol Chem. 258 (1): 636-42. -
Ulrich, R. et al. (2010) Machine learning approach identifies new pathways associated with demyelination in a viral model of multiple sclerosis.
J Cell Mol Med. 14 (1-2): 434-48. -
Amirbekian, V. et al. (2007) Detecting and assessing macrophages in vivo to evaluate atherosclerosis noninvasively using molecular MRI.
Proc Natl Acad Sci U S A. 104: 961-6. -
Fan, D. et al. (2014) Differential role of TIMP2 and TIMP3 in cardiac hypertrophy, fibrosis, and diastolic dysfunction.
Cardiovasc Res. 103 (2): 268-80. -
Higuchi, Y. et al. (2012) Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.
Am J Physiol Heart Circ Physiol. 302: H2352-62. -
Ishibashi, M. et al. (2004) Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling.
Circ Res. 94: 1203-10.
View The Latest Product References
-
Sato, A. et al. (2008) Thioredoxin-1 ameliorates cigarette smoke-induced lung inflammation and emphysema in mice.
J Pharmacol Exp Ther. 325: 380-8. -
Xu, J. et al. (2007) Role of cardiac overexpression of ANG II in the regulation of cardiac function and remodeling postmyocardial infarction.
Am J Physiol Heart Circ Physiol. 293: H1900-7. -
Zhao, Q. et al. (2004) Essential role of vascular endothelial growth factor in angiotensin II-induced vascular inflammation and remodeling.
Hypertension. 44: 264-70. -
Hansmann, F. et al. (2012) Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system.
Cell Transplant. 21 (6): 1161-75. -
Herder, V. et al. (2015) Dynamic Changes of Microglia/Macrophage M1 and M2 Polarization in Theiler's Murine Encephalomyelitis.
Brain Pathol. 25 (6): 712-23. -
Bröer S et al. (2016) Brain inflammation, neurodegeneration and seizure development following picornavirus infection markedly differ among virus and mouse strains and substrains.
Exp Neurol. pii: S0014-4886(16)30033-4. -
Bobbala, D. et al. (2016) Interleukin-21-dependent modulation of T cell antigen receptor reactivity towards low affinity peptide ligands in autoreactive CD8(+) T lymphocytes.
Cytokine. 85: 83-91. -
Raddatz, B.B. et al. (2016) Central Nervous System Demyelination and Remyelination is Independent from Systemic Cholesterol Level in Theiler's Murine Encephalomyelitis.
Brain Pathol. 26 (1): 102-19. -
Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
Brain Pathol. 28 (3): 349-368. -
Fayyad, A. et al. (2018) Matrix metalloproteinases expression in spontaneous canine histiocytic sarcomas and its xenograft model.
Vet Immunol Immunopathol. 198: 54-64. -
Li, L. et al. (2015) Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
J Neuroinflammation. 12: 242. -
Hansmann, F. et al. (2018) Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
Vet Immunol Immunopathol. 202: 130-40. -
Armando, F. et al. (2021) Intratumoral Canine Distemper Virus Infection Inhibits Tumor Growth by Modulation of the Tumor Microenvironment in a Murine Xenograft Model of Canine Histiocytic Sarcoma.
Int J Mol Sci. 2 (7): 3578. -
Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
Brain Pathol. 28 (3): 349-68. -
Waltl, I. et al. (2018) Microglia have a protective role in viral encephalitis-induced seizure development and hippocampal damage.
Brain Behav Immun. 74: 186-204. -
Wiedemann, G.M. et al. (2016) A novel TLR7 agonist reverses NK cell anergy and cures RMA-S lymphoma-bearing mice.
Oncoimmunology. 5 (7): e1189051. -
Dey, S. et al. (2021) Loss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis.
JCI Insight. 6 (19): e149539. -
Iwaki, T. et al. (2021) Plasminogen Deficiency Significantly Reduces Vascular Wall Disease in a Murine Model of Type IIa Hypercholesterolemia
Biomedicines. 9 (12): 1832. -
Bühler, M. et al. (2023) IFNAR signaling of neuroectodermal cells is essential for the survival of C57BL/6 mice infected with Theiler's murine encephalomyelitis virus.
J Neuroinflammation. 20 (1): 58. -
Avloniti, M. et al. (2024) IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severity.
J Neuroinflammation. 21 (1): 34. -
Siddiqui, J.A. et al. (2021) Osteoblastic monocyte chemoattractant protein-1 (MCP-1) mediation of parathyroid hormone's anabolic actions in bone implicates TGF-β signaling.
Bone. 143: 115762. -
Beute, J. et al. (2020) PDE3 Inhibition Reduces Epithelial Mast Cell Numbers in Allergic Airway Inflammation and Attenuates Degranulation of Basophils and Mast Cells.
Front Pharmacol. 11: 470.
- Synonyms
- MAC-3
- RRID
- AB_2134762
- UniProt
- P17047
- Entrez Gene
- Lamp2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0005770 late endosome
- GO:0010008 endosome membrane
- GO:0030670 phagocytic vesicle membrane
MCA2293GA
160239
1607
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