CD107b antibody | M3/84
Rat anti Mouse CD107b antibody, clone M3/84 (MCA2293) used for the identification of MAC-3 expressing cells in mouse spinal cord sections by immunofluorescence.
Image caption:
ISG15 and PKR protein expression of astrocytes, oligodendrocytes, and microglia/macrophages in spinal cord white matter lesions at 98 dpi. ISG15 (green) is expressed by astrocytes (red, a), oligodendrocytes (red, c), and microglia/macrophages (red, e) demonstrated by an orange-yellow mixed color (arrows). No co-localization of PKR (green, arrowheads) with GFAP (red, arrows) indicates no/low PKR expression in astrocytes (red, b), whereas co-localization of PKR (green) with Olig2 (red, d) and MAC-3 (red, f) demonstrates PKR expression in oligodendrocytes and microglia (arrows). Immunofluorescence double-staining for ISG15 and PKR with GFAP, Olig2, and MAC-3. Nuclei are stained with bisbenzimide. Bar 25μm.
From: Li L, Ulrich R, Baumgärtner W, Gerhauser I.
Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
J Neuroinflammation. 2015 Dec 24;12:242.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. Studies suggest that CD107b may be involved in leucocyte adhesion.
The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining.
The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Glycoproteins purified from mouse peritoneal macrophage membranes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P17047 Related reagents
- Entrez Gene
- Lamp2 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0005770 late endosome
- GO:0010008 endosome membrane
- GO:0030670 phagocytic vesicle membrane
- Regulatory
- For research purposes only
Applications of CD107b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/50 | 1/100 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Western Blotting |
- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for CD107b antibody
-
Springer, T.A. (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.
J Biol Chem. 256 (8): 3833-9. -
Flotte, T.J. et al. (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets.
Am J Pathol. 111 (1): 112-24. -
Ho, M.K. & Springer, T.A. (1983) Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity.
J Biol Chem. 258 (1): 636-42. -
Ulrich, R. et al. (2010) Machine learning approach identifies new pathways associated with demyelination in a viral model of multiple sclerosis.
J Cell Mol Med. 14 (1-2): 434-48. -
Amirbekian, V. et al. (2007) Detecting and assessing macrophages in vivo to evaluate atherosclerosis noninvasively using molecular MRI.
Proc Natl Acad Sci U S A. 104: 961-6. -
Fan, D. et al. (2014) Differential role of TIMP2 and TIMP3 in cardiac hypertrophy, fibrosis, and diastolic dysfunction.
Cardiovasc Res. 103 (2): 268-80. -
Higuchi, Y. et al. (2012) Upregulation of anticoagulant proteins, protein S and tissue factor pathway inhibitor, in the mouse myocardium with cardio-specific TNF-α overexpression.
Am J Physiol Heart Circ Physiol. 302: H2352-62. -
Ishibashi, M. et al. (2004) Critical role of monocyte chemoattractant protein-1 receptor CCR2 on monocytes in hypertension-induced vascular inflammation and remodeling.
Circ Res. 94: 1203-10. -
Sato, A. et al. (2008) Thioredoxin-1 ameliorates cigarette smoke-induced lung inflammation and emphysema in mice.
J Pharmacol Exp Ther. 325: 380-8. -
Xu, J. et al. (2007) Role of cardiac overexpression of ANG II in the regulation of cardiac function and remodeling postmyocardial infarction.
Am J Physiol Heart Circ Physiol. 293: H1900-7. -
Zhao, Q. et al. (2004) Essential role of vascular endothelial growth factor in angiotensin II-induced vascular inflammation and remodeling.
Hypertension. 44: 264-70. -
Hansmann, F. et al. (2012) Highly malignant behavior of a murine oligodendrocyte precursor cell line following transplantation into the demyelinated and nondemyelinated central nervous system.
Cell Transplant. 21 (6): 1161-75. -
Herder, V. et al. (2015) Dynamic Changes of Microglia/Macrophage M1 and M2 Polarization in Theiler's Murine Encephalomyelitis.
Brain Pathol. 25 (6): 712-23. -
Bröer S et al. (2016) Brain inflammation, neurodegeneration and seizure development following picornavirus infection markedly differ among virus and mouse strains and substrains.
Exp Neurol. pii: S0014-4886(16)30033-4. -
Bobbala, D. et al. (2016) Interleukin-21-dependent modulation of T cell antigen receptor reactivity towards low affinity peptide ligands in autoreactive CD8(+) T lymphocytes.
Cytokine. 85: 83-91. -
Raddatz, B.B. et al. (2016) Central Nervous System Demyelination and Remyelination is Independent from Systemic Cholesterol Level in Theiler's Murine Encephalomyelitis.
Brain Pathol. 26 (1): 102-19. -
Ciurkiewicz, M. et al. (2018) Cytotoxic CD8+ T cell ablation enhances the capacity of regulatory T cells to delay viral elimination in Theiler's murine encephalomyelitis.
Brain Pathol. 28 (3): 349-368. -
Fayyad, A. et al. (2018) Matrix metalloproteinases expression in spontaneous canine histiocytic sarcomas and its xenograft model.
Vet Immunol Immunopathol. 198: 54-64. -
Li, L. et al. (2015) Interferon-stimulated genes-essential antiviral effectors implicated in resistance to Theiler's virus-induced demyelinating disease.
J Neuroinflammation. 12: 242.
