CD105 antibody | MJ7/18

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MCA4706
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C F IP P WB 0.25 mg loader
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Rat anti Mouse CD105 antibody, clone MJ7/18 recognizes mouse CD105, also known as Endoglin, a cell surface glycoprotein member of the TGF beta receptor complex. CD105 is a homodimer that forms a heteromeric complex with TGF beta receptors I and/or II. The complex binds TGF beta 1 and 3 efficiently, and TGF beta 2 less efficiently. It may also play a role in the binding of endothelial cells to integrins and cytoskeletal organisation. It is involved in cardiovascular development. CD105 is expressed on endothelial cells in all tissues except bone marrow and in also found in stromal cells within the connective tissue of the intestine, stomach and heart. It may be a useful marker for proliferating endothelium in tumours.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Inflamed mouse skin.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD105 antibody

  1. Ge, A.Z. & Butcher, E.C. (1994) Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand.
    Gene. 138 (1-2): 201-6.
  2. Hallmann, R. et al. (1995) Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Dev Dyn. 202 (4): 325-32.
  3. Bredow, S. et al. (2000) Imaging of tumour neovasculature by targeting the TGF-beta binding receptor endoglin.
    Eur J Cancer. 36 (5): 675-81.
  4. Leguerney, I. et al. (2015) Molecular ultrasound imaging using contrast agents targeting endoglin, vascular endothelial growth factor receptor 2 and integrin.
    Ultrasound Med Biol. 41 (1): 197-207.
  5. Crouch, E.E. et al. (2015) Regional and Stage-Specific Effects of Prospectively Purified Vascular Cells on the Adult V-SVZ Neural Stem Cell Lineage.
    J Neurosci. 35 (11): 4528-39.
  6. Işık, S. et al. (2016) Intraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine model.
    Eur Arch Otorhinolaryngol. Jul 5. [Epub ahead of print]
  7. Winkler, S. et al. (2015) Mouse white adipose tissue-derived mesenchymal stem cells gain pericentral and periportal hepatocyte features after differentiation in vitro, which are preserved in vivo after hepatic transplantation.
    Acta Physiol (Oxf). 215 (2): 89-104.

Immunofluorescence

Synonyms
Endoglin
RRID
AB_2098891
UniProt
Q63961
Entrez Gene
Eng
GO Terms
GO:0001300 chronological cell aging
GO:0001569 patterning of blood vessels
GO:0001947 heart looping
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0050431 transforming growth factor beta binding
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0042803 protein homodimerization activity
GO:0048745 smooth muscle tissue development
GO:0048844 artery morphogenesis
GO:0048845 venous blood vessel morphogenesis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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