CD105 antibody | MJ7/18

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Rat anti Mouse CD105

Product Type
Monoclonal Antibody
Clone
MJ7/18
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4706 C F IP P WB 0.25 mg
Rat anti Mouse CD105 antibody, clone MJ7/18 recognizes mouse CD105, also known as Endoglin, a cell surface glycoprotein member of the TGF beta receptor complex. CD105 is a homodimer that forms a heteromeric complex with TGF beta receptors I and/or II. The complex binds TGF beta 1 and 3 efficiently, and TGF beta 2 less efficiently. It may also play a role in the binding of endothelial cells to integrins and cytoskeletal organisation. It is involved in cardiovascular development. CD105 is expressed on endothelial cells in all tissues except bone marrow and in also found in stromal cells within the connective tissue of the intestine, stomach and heart. It may be a useful marker for proliferating endothelium in tumours.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Inflamed mouse skin.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q63961 Related reagents
Entrez Gene
Eng Related reagents
GO Terms
GO:0001300 chronological cell aging
GO:0001569 patterning of blood vessels
GO:0001947 heart looping
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0050431 transforming growth factor beta binding
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0042803 protein homodimerization activity
GO:0048745 smooth muscle tissue development
GO:0048844 artery morphogenesis
GO:0048845 venous blood vessel morphogenesis
Regulatory
For research purposes only

Applications of CD105 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control MCA1212 1 ml E F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Immunofluorescence

Product Specific References

References for CD105 antibody

  1. Ge, A.Z. & Butcher, E.C. (1994) Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand.
    Gene. 138 (1-2): 201-6.
  2. Hallmann, R. et al. (1995) Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier.
    Dev Dyn. 202 (4): 325-32.
  3. Bredow, S. et al. (2000) Imaging of tumour neovasculature by targeting the TGF-beta binding receptor endoglin.
    Eur J Cancer. 36 (5): 675-81.
  4. Leguerney, I. et al. (2015) Molecular ultrasound imaging using contrast agents targeting endoglin, vascular endothelial growth factor receptor 2 and integrin.
    Ultrasound Med Biol. 41 (1): 197-207.
  5. Crouch, E.E. et al. (2015) Regional and Stage-Specific Effects of Prospectively Purified Vascular Cells on the Adult V-SVZ Neural Stem Cell Lineage.
    J Neurosci. 35 (11): 4528-39.
  6. Işık, S. et al. (2016) Intraperitoneal mesenchymal stem cell administration ameliorates allergic rhinitis in the murine model.
    Eur Arch Otorhinolaryngol. Jul 5. [Epub ahead of print]
  7. Winkler, S. et al. (2015) Mouse white adipose tissue-derived mesenchymal stem cells gain pericentral and periportal hepatocyte features after differentiation in vitro, which are preserved in vivo after hepatic transplantation.
    Acta Physiol (Oxf). 215 (2): 89-104.