CD103 antibody | 2E7

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Hamster anti Mouse CD103:FITC

Hamster anti Mouse CD103

Hamster anti Mouse CD103:RPE

Product Type
Monoclonal Antibody
Clone
2E7
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4705F F 0.1 mg
MCA4705GA C F IF IP 0.1 mg
MCA4705PE F 0.1 mg
Hamster anti Mouse CD103 antibody, clone 2E7 recognizes mouse CD103, otherwise known as integrin alpha-E. CD103 binds Integrin beta 7 to form the heterodimeric integrin molecule αEβ7. This complex is sometimes referred to as CD103, though strictly speaking CD103 only refers to the alpha chain. The beta 7 subunit can also interact with other integrin alpha chains. The αEβ7 complex is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocyes to epithelial cell monolayers. CD103 is expressed on intestinal intraepithelial lymphocytes and is highly expressed on regulatory T cells. Hamster anti Mouse CD103 antibody, clone 2E7 is expected to also recognize the intact αEβ7 complex.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse intestinal intraepithelial lymphocytes
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q60677 Related reagents
Entrez Gene
Itgae Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
Regulatory
For research purposes only

Applications of CD103 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5
Flow Cytometry 1/10 1/20
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F 0.1 mg F
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F
Hamster (Armenian) IgG Negative Control:RPE MCA2356PE 0.1 mg F

Product Specific References

References for CD103 antibody

  1. Lefran├žois, L. et al. (1994) Developmental expression of the alpha IEL beta 7 integrin on T cell receptor gamma delta and T cell receptor alpha beta T cells.
    Eur J Immunol. 24: 635- 640