Ipilimumab antibody | AbD34294
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ipilimumab 9Drug/Target complex) Antibody, clone AbD34294pap (TZA001P) at a concentration of 0.2 μg/ml and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with CTLA-4, ipilimumab, human IgG1 kappa or human IgG1 lambda at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, the CTLA-4/ipilimumab complex was formed by adding 5 µg/ml ipilimumab to the wells coated with 5 µg/m CTLA-4. Detection was performed using Human Anti-Ipilimumab (Drug/Target complex) Antibody, clone AbD34294pap (TZA001P) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human CTLA-4 at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, 10% Human Serum was added spiked with increasing concentrations of ipilimumab. Direct or indirect detection was performed using Human Anti-Ipilimumab (Drug/Target complex) Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Direct detection used HRP labeled Human Anti-Ipilimumab (Drug/Target complex) Antibody, clone AbD34294pap (TZA001P) at a concentration of 0.2 µg/ml, or indirect detection used antibody clone AbD34294ad (TZA001) at a concentration of 2 µg/ml followed by an HRP labeled anti-DYKDDDDK tag antibody. Data are shown as the mean of three measurements.
Human anti Ipilimumab (Drug/Target Complex):HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD34294
- Isotype
- Fab antibody
- Specificity
- Ipilimumab
- Region
- DRUG/TARGET COMPLEX
Antibody Quality and Performance Guarantee
| Human Anti-Ipilimumab (Drug/Target Complex) Antibody, clone AbD34294 specifically recognizes the antibody drug ipilimumab when in complex with its target, CTLA-4 (cytotoxic T-lymphocyte antigen-4). The antibody does not recognize free ipilimumab nor unbound recombinant human CTLA-4. It can be used to measure levels of ipilimumab in patient samples. This TrailBlazer Antibody is available in the SpyTag monovalent recombinant Fab format (TZA001), and the bivalent SpyTag Fab format (TZA001P) coupled with a HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free ipilimumab captured via immobilized CTLA-4. Protocol: PK Antigen Capture ELISA Anti-Ipilimumab monovalent Fab antibody TZA001 can also be converted by the user into alternative antibody formats using any of the SpyCatchers available in our catalog. View a summary of all SpyCatcher products Ipilimumab (Yervoy) is a human IgG1/kappa antibody developed from a transgenic mouse. It has been approved for the treatment of metastatic melanoma, renal cell carcinoma, and in combination with nivolumab for the treatment of previously treated microsatellite instability-high/deficient mismatch repair (MSI-H/dMMR) metastatic colorectal cancer. Ipilimumab activates the immune system by targeting CTLA-4, a protein receptor that downregulates the immune system. The action of cytotoxic T lymphocytes (CTLs) to recognize and destroy cancer cells is subject to an inhibitory mechanism that interrupts this destruction. Ipilimumab turns off this inhibitory mechanism and allows CTLs to function. View a summary of all anti-ipilimumab antibodies |
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- Product Form
- A bivalent human recombinant Fab (kappa light chain) generated by coupling of the monovalent SpyTagged Fab TZA001 with a HRP conjugated BiSpyCatcher version 2. The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Ipilimumab/CTLA-4 complex
- Affinity
- The monovalent intrinsic affinity of AbD34294pap was measured as KD = 252 nM by real time, label free molecular interaction analysis on immobilized CTLA-4/ipilimumab complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Yervoy is a trademark of Bristol-Myers Squibb Company.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- More information about SpyTag technology can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD34294pap is recommended for use as the detection reagent in an antigen capture assay to measure free ipilimumab captured via immobilized CTLA-4.
Protocol: PK antigen capture ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyTag3 Peptide | BLP086 | IY | 1 mg |
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| Description | SpyTag3 Peptide | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Ipilimumab | HCA327 | E | 0.1 mg |
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| Description | Human anti Ipilimumab | ||||||
| Human anti Ipilimumab | HCA328 | E | 0.1 mg |
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| Description | Human anti Ipilimumab | ||||||
| Human anti Ipilimumab | HCA329 | E | 0.1 mg |
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| Description | Human anti Ipilimumab | ||||||
| Human anti Ipilimumab | HCA330 | E | 0.1 mg |
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| Description | Human anti Ipilimumab | ||||||
| Human anti Ipilimumab (Drug/Target Complex) | HCA331 | E | 0.1 mg |
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| Description | Human anti Ipilimumab (Drug/Target Complex) | ||||||
| Human anti Ipilimumab (Drug/Target Complex) | TZA001 | E | 0.1 mg | Log in | |||
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| Description | Human anti Ipilimumab (Drug/Target Complex) | ||||||
- Synonyms
- Yervoy
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
TZA001P
165521
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