Ipilimumab antibody | AbD34428ia
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E ResetHuman anti Ipilimumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD34428ia
- Isotype
- IgG1
- Specificity
- Ipilimumab
Human Anti-Ipilimumab Antibody, clone AbD34428ia is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody ipilimumab. It does not recognize free human CTLA-4 (cytotoxic T-lymphocyte antigen-4) or ipilimumab in complex with CTLA-4. Clone AbD34428ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 0.3 nM, and is one of three antibodies with different affinities offered for ADA assays. Antibody HCA327 has affinity 16 nM and HCA329 has affinity 1 nM. Ipilimumab (Yervoy) is a human IgG1/kappa antibody developed from a transgenic mouse. It has been approved by the FDA for the treatment of metastatic melanoma, renal cell carcinoma, and in combination with nivolumab for the treatment of previously treated microsatellite instability-high/deficient mismatch repair (MSI-H/dMMR) metastatic colorectal cancer. Ipilimumab activates the immune system by targeting CTLA-4, a protein receptor that downregulates the immune system. The action of cytotoxic T lymphocytes (CTLs) to recognize and destroy cancer cells is subject to an inhibitory mechanism that interrupts this destruction. Ipilimumab turns off this inhibitory mechanism and allows CTLs to function. View a summary of all anti-ipilimumab antibodies |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Ipilimumab
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Ipilimumab antibody, clone AbD34428ia is KD = 0.3 nM as measured by real time, label free molecular interaction analysis on immobilized Ipilimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Yervoy is a trademark of Bristol-Myers Squibb Company. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Ipilimumab antibody, clone AbD34428ia can be used in an indirect ELISA. It can also be used as a surrogate positive control or calibrator in an anti-drug antibody assay.
Protocol: ADA bridging ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Ipilimumab | HCA327 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Ipilimumab | ||||||
Human anti Ipilimumab | HCA329 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Ipilimumab | ||||||
Human anti Ipilimumab | HCA330 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Ipilimumab | ||||||
Human anti Ipilimumab (Drug/Target Complex) | HCA331 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Ipilimumab (Drug/Target Complex) | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | LYNX Rapid HRP Antibody Conjugation Kit |
- Synonyms
- Yervoy
HCA328
153335 157343 157708 164506If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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