Infliximab antibody | AbD17841_hIgG1
Human anti Infliximab
- Product Type
- Monoclonal Antibody
- Clone
- AbD17841_hIgG1
- Isotype
- IgG1
- Specificity
- Infliximab
Human Anti-Infliximab, clone AbD17841_hIgG1 is an anti-idiotypic antibody that specifically recognizes the infliximab monoclonal antibody and inhibits the binding of infliximab to its target. It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products, such as Inflectra and Renflexis, in patient samples. Human anti Infliximab, clone AbD17841_hIgG1 is a fully human antibody and may be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera. In the HRP-conjugated format, clone AbD17841_IgG1 is suitable as a detection antibody in pharmacokinetic (PK) assays. Infliximab (branded as Remicade) is a chimeric monoclonal antibody drug (IgG1/kappa) that has been approved for treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, and ulcerative colitis. Infliximab is directed against Tumor Necrosis Factor Alpha (TNFα) and acts by blocking the binding of this cytokine to its receptors. Infliximab also induces apoptosis of TNFα expressing T-lymphocytes. View a summary of all Anti-Infliximab Antibodies. |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Infliximab
- Affinity
- The monovalent intrinsic affinity of this antibody is KD=1.8 nM as measured by real time, label-free molecular interaction analysis on immobilized Infliximab.
- Approx. Protein Concentrations
- HCA213G: Antibody concentration 1.0 mg/ml
- HCA213: Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Remicade is a trademark of Janssen Biotech Inc. Inflectra is a trademark of Hospira, a Pfizer Company. RENFLEXIS is a trademark of Merck Sharp & Dohme Corp. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in a direct ELISA, as a bridging antibody in ADA assays or as a detection antibody in an Infliximab bridging ELISA when conjugated to HRP together with HCA212 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
Protocol: ADA bridging ELISA.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Infliximab | HCA212 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA214 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA215 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA216 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Infliximab | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
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Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C * E | 0.2 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Human IgG (Fc) CH2 Domain:HRP | ||||||
Recombinant Human TNF Alpha | PHP051 | E FN WB | 50 µg | Log in | |||
List Price | Your Price | ||||||
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Description | Recombinant Human TNF Alpha |
References for Infliximab antibody
-
Hernández-Breijo, B. et al. (2016) Standardization of the homogeneous mobility shift assay protocol for evaluation of anti-infliximab antibodies. Application of the method to Crohn's disease patients treated with infliximab.
Biochem Pharmacol. Sep 21. pii: S0006-2952(16)30302-1. [Epub ahead of print]
- Synonyms
- Remicade
HCA213G
1709HCA213
150980 1612 163092If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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