TOPBP1 antibody | E03/6C8

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Mouse anti TOPBP1

Product Type
PrecisionAb Monoclonal
Clone
E03/6C8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00940
Datasheet
IP WB
SDS
100 µl loader

Mouse anti TOPBP1 antibody recognizes DNA topoisomerase 2-binding protein 1 (TOPBP1). TOPBP1 is a large, non-enzymatic scaffold protein that plays essential roles in many cellular processes including DNA repair, replication, and transcription. The nine BRCT domains of TOPBP1 allow TOPBP1 to bind to numerous interacting proteins (Moudry et al. 2016). TOPBP1 mediates the response to DNA damage through binding to MDC1. This binding allows TOPBP1 recruitment to double-strand breaks (DSBs) in mitosis (Leimbacher et al. 2019). The role of TOPBP1 in DNA processing during mitosis is important for genome integrity, preventing DNA damage from being passed to daughter cells (Gallina et al. 2016). Due to its role in genome integrity, it is unsurprising that dysregulation of TOPBP1 has been identified in cancer. For example, TOPBP1 has been found to be elevated in a range of sarcoma types. In osteosarcoma, TOPBP1 appears to contribute to cancer cell survival and chemoresistance to doxorubicin and cisplatin (Wu et al. 2017). In non-small cell lung cancer, TOPBP1 contributes to chemoresistance by upregulating the transcription factor p53 (Lv et al. 2016).

Product Details

Target Species
Human
Western Blotting
Mouse anti TOPBP1 detects a band of approximately 198 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1097-1522 of human TOPBP1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q92547
Entrez Gene
TOPBP1
GO Terms
GO:0000922 spindle pole
GO:0003677 DNA binding
GO:0005815 microtubule organizing center
GO:0006281 DNA repair
GO:0008022 protein C-terminus binding
GO:0010212 response to ionizing radiation
GO:0016605 PML body
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of TOPBP1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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