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TNF Alpha antibody | MP9-20A4

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Anti Human TNF Alpha Antibody, clone MP9-20A4 thumbnail image 1 Anti Human TNF Alpha Antibody, clone MP9-20A4 thumbnail image 2 Anti Human TNF Alpha Antibody, clone MP9-20A4 thumbnail image 3
Anti Human TNF Alpha Antibody, clone MP9-20A4 gallery image 1
Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat anti Human TNF alpha (MCA1560PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat IgG1 (MCA6004PE). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and PE conjugated Rat anti Human TNF alpha (MCA1560PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human TNF Alpha Antibody, clone MP9-20A4 gallery image 2
Figure A. Unstimulated cells were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat anti Human TNF alpha (MCA1560) detected with PE conjugated Goat anti Rat (STAR73). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat IgG1 (MCA6004) detected with PE conjugated Goat anti Rat (STAR73). Figure C. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 6 hours were stained with FITC conjugated Mouse anti Human CD3 (MCA463F) and purified Rat anti Human TNF alpha (MCA1560) detected with PE conjugated Goat anti Rat (STAR73). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human TNF Alpha Antibody, clone MP9-20A4 gallery image 3
Published customer image:
Mouse anti Human TNF-&alpha antibody, clone MP9-20A4 (MCA1560) used for the evaluation of TNF-α expression by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Representative staining patterns of the protein expression of TNF-α in primary tumors of patients with benign thyroid diseases and PTC.

From: Kobawala, T.P. et a;
Significance of TNFα and the adhesion molecules: L-selectin and VCAM-1 in Papillary Thyroid Carcinoma.
J Thyroid Res. 2016 Article ID8143695.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Rat anti Human TNF Alpha:RPE

Product Type
Monoclonal Antibody
Clone
MP9-20A4
Isotype
IgG1
Specificity
TNF Alpha

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Product Code Applications Pack Size List Price Your Price Qty
MCA1560PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests
List Price Your Price

Rat anti Human TNF alpha antibody, clone MP9-20A4 recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.

Rat anti Human TNF alpha antibody, clone MP9-20A4 is reported to act as a blocking antibody (Abrams et al. 2001).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend micro-centrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG1 Negative Control:RPE MCA6004PE F 100 Tests
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Description Rat IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for TNF Alpha antibody

  1. Butler, D.M. et al. (1997) DBA/1 mice expressing the human TNF-alpha transgene develop a severe, erosive arthritis: characterization of the cytokine cascade and cellular composition.
    J Immunol. 159 (6): 2867-76.
  2. Abrams, J.S. (2001) Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.
    Curr Protoc Immunol. Chapter 6: Unit 6.20.
  3. Evans HG et al. (2009) In vivo activated monocytes from the site of inflammation in humans specifically promote Th17 responses.
    Proc Natl Acad Sci U S A. 106 (15): 6232-7.
  4. Vujanovic, L. et al. (2010) Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.
    Blood. 116: 575-83.
  5. Kumagai, M. et al. (2012) Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.
    J Control Release. 160 (3): 542-51.
  6. Poussin C et al. (2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis.
    Toxicol In Vitro. 28 (5): 896-908.
  7. Mann, E.R. et al. (2016) Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.
    Gut. 65 (2): 256-70.
  8. Kobawala TP et al. (2016) Significance of TNF-α and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma.
    J Thyroid Res. 2016: 8143695.
    9. View The Latest Product References

Further Reading

  1. Kadowaki, N. et al. (2000) Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    J Exp Med. 192 (2): 219-26.

Flow Cytometry

RRID
AB_322497
UniProt
P01375
Entrez Gene
TNF
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0000185 activation of MAPKKK activity
GO:0000187 activation of MAPK activity
GO:0001891 phagocytic cup
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0009615 response to virus
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0006927 transformed cell apoptosis
GO:0050796 regulation of insulin secretion
GO:0042802 identical protein binding
GO:0009651 response to salt stress
GO:0009897 external side of plasma membrane
GO:0010843 promoter binding
GO:0010888 negative regulation of lipid storage
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030730 sequestering of triglyceride
GO:0031334 positive regulation of protein complex assembly
GO:0031622 positive regulation of fever generation
GO:0032582 negative regulation of gene-specific transcription
GO:0032715 negative regulation of interleukin-6 production
GO:0032722 positive regulation of chemokine production
GO:0032800 receptor biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034116 positive regulation of heterotypic cell-cell adhesion
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043193 positive regulation of gene-specific transcription
GO:0043243 positive regulation of protein complex disassembly
GO:0045071 negative regulation of viral genome replication
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045121 membrane raft
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045672 positive regulation of osteoclast differentiation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050715 positive regulation of cytokine secretion
GO:0050901 leukocyte tethering or rolling
GO:0050995 negative regulation of lipid catabolic process
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0055037 recycling endosome
GO:0060555 induction of necroptosis by extracellular signals
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0061048 negative regulation of branching involved in lung morphogenesis
GO:0070265 necrotic cell death
GO:0070374 positive regulation of ERK1 and ERK2 cascade

MCA1560PE

INN1610

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