TNF Alpha antibody | MP9-20A4

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		5ug/ml	10ug/ml
Flow Cytometry 1
Immunohistology - Frozen
Western Blotting

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

References for TNF Alpha antibody

1. Butler, D.M. et al. (1997) DBA/1 mice expressing the human TNF-alpha transgene develop a severe, erosive arthritis: characterization of the cytokine cascade and cellular composition.
   J Immunol. 159 (6): 2867-76.
2. Abrams, J.S. (2001) Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.
   Curr Protoc Immunol. Chapter 6: Unit 6.20.
3. Evans HG et al. (2009) In vivo activated monocytes from the site of inflammation in humans specifically promote Th17 responses.
   Proc Natl Acad Sci U S A. 106 (15): 6232-7.
4. Vujanovic, L. et al. (2010) Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.
   Blood. 116: 575-83.
5. Kumagai, M. et al. (2012) Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.
   J Control Release. 160 (3): 542-51.
6. Poussin C et al. (2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis.
   Toxicol In Vitro. 28 (5): 896-908.
7. Mann, E.R. et al. (2016) Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.
   Gut. 65 (2): 256-70.
8. Kobawala TP et al. (2016) Significance of TNF-α and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma.
   J Thyroid Res. 2016: 8143695.
View The Latest Product References

Further Reading

1. Kadowaki, N. et al. (2000) Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
   J Exp Med. 192 (2): 219-26.

Flow Cytometry

RRID

AB_567239

UniProt

P01375

Entrez Gene

TNF

GO Terms

GO:0000060 protein import into nucleus, translocation

GO:0000185 activation of MAPKKK activity

GO:0000187 activation of MAPK activity

GO:0001891 phagocytic cup

GO:0002439 chronic inflammatory response to antigenic stimulus

GO:0002740 negative regulation of cytokine secretion involved in immune response

GO:0009615 response to virus

GO:0005887 integral to plasma membrane

GO:0005125 cytokine activity

View More GO Terms

GO:0005164 tumor necrosis factor receptor binding

GO:0005615 extracellular space

GO:0006916 anti-apoptosis

GO:0006919 activation of caspase activity

GO:0006927 transformed cell apoptosis

GO:0050796 regulation of insulin secretion

GO:0042802 identical protein binding

GO:0009651 response to salt stress

GO:0009897 external side of plasma membrane

GO:0010843 promoter binding

GO:0010888 negative regulation of lipid storage

GO:0016563 transcription activator activity

GO:0016564 transcription repressor activity

GO:0030730 sequestering of triglyceride

GO:0031334 positive regulation of protein complex assembly

GO:0031622 positive regulation of fever generation

GO:0032582 negative regulation of gene-specific transcription

GO:0032715 negative regulation of interleukin-6 production

GO:0032722 positive regulation of chemokine production

GO:0032800 receptor biosynthetic process

GO:0033138 positive regulation of peptidyl-serine phosphorylation

GO:0033209 tumor necrosis factor-mediated signaling pathway

GO:0034116 positive regulation of heterotypic cell-cell adhesion

GO:0042346 positive regulation of NF-kappaB import into nucleus

GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade

GO:0043193 positive regulation of gene-specific transcription

GO:0043243 positive regulation of protein complex disassembly

GO:0045071 negative regulation of viral genome replication

GO:0045080 positive regulation of chemokine biosynthetic process

GO:0045121 membrane raft

GO:0045429 positive regulation of nitric oxide biosynthetic process

GO:0045599 negative regulation of fat cell differentiation

GO:0045672 positive regulation of osteoclast differentiation

GO:0048661 positive regulation of smooth muscle cell proliferation

GO:0050715 positive regulation of cytokine secretion

GO:0050901 leukocyte tethering or rolling

GO:0050995 negative regulation of lipid catabolic process

GO:0051044 positive regulation of membrane protein ectodomain proteolysis

GO:0051092 positive regulation of NF-kappaB transcription factor activity

GO:0051384 response to glucocorticoid stimulus

GO:0055037 recycling endosome

GO:0060555 induction of necroptosis by extracellular signals

GO:0060557 positive regulation of vitamin D biosynthetic process

GO:0060559 positive regulation of calcidiol 1-monooxygenase activity

GO:0061048 negative regulation of branching involved in lung morphogenesis

GO:0070265 necrotic cell death

GO:0070374 positive regulation of ERK1 and ERK2 cascade

MCA1560

0314 157739

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