SMARCA4 antibody | 5B7
Rat anti Human SMARCA4
- Product Type
- Monoclonal Antibody
- Clone
- 5B7
- Isotype
- IgG2a
- Specificity
- SMARCA4
Antibody Quality and Performance Guarantee
| Rat anti Human SMARCA4 antibody, clone 5B7 recognizes special AT-rich sequence-binding protein 2, also known as transcription activator BRG1. SMARCA4 is a member of the SWI/SNF family of proteins. SWI/SNF proteins display ATPase and helicase activities and regulate gene transcription via chromatin remodelling. SMRCA4 is important for spermatogenesis and embryo development (Kim et al. 2012) (Zhang et al. 2011). SMARCA4 is also the most frequently mutated chromatin remodeling ATPase in cancer. Mutations in the SMARCA4 have been observed in many tumor subtypes including pancreatic cancers and some types of leukemia (Jones et al. 2012) (Shain et al. 2012) (Hodges et al. 2018) (Romero et al. 2012). Rat anti Human SMARCA4 antibody detects a band of 180 kDa. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A recombinant protein corresponding to amino acids 213-295 of human SMARCA4
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
2 ug/ml | 10 ug/ml |
| Western Blotting | |
0.5 ug/ml | 2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P51532
- Entrez Gene
- SMARCA4
- GO Terms
- GO:0002039 p53 binding
- GO:0003677 DNA binding
- GO:0003714 transcription corepressor activity
- GO:0004386 helicase activity
- GO:0005524 ATP binding
- GO:0006338 chromatin remodeling
- GO:0007399 nervous system development
- GO:0008094 DNA-dependent ATPase activity
- GO:0042802 identical protein binding
- View More GO Terms
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0016514 SWI/SNF complex
- GO:0016563 transcription activator activity
- GO:0045749 negative regulation of S phase of mitotic cell cycle
- GO:0047485 protein N-terminus binding
- GO:0051091 positive regulation of transcription factor activity
- GO:0070577 histone acetyl-lysine binding
- GO:0071564 npBAF complex
- GO:0071565 nBAF complex
- GO:0071778 WINAC complex
MCA6384
160854
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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