SMARCA4 antibody | 5B7

Rat anti Human SMARCA4

Product Type
Monoclonal Antibody
Clone
5B7
Isotype
IgG2a
Specificity
SMARCA4

Product Code Applications Pack Size List Price Your Price Qty
MCA6384
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 0.1 mg loader
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loader
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Rat anti Human SMARCA4 antibody, clone 5B7 recognizes special AT-rich sequence-binding protein 2, also known as transcription activator BRG1.

SMARCA4 is a member of the SWI/SNF family of proteins. SWI/SNF proteins display ATPase and helicase activities and regulate gene transcription via chromatin remodelling. SMRCA4 is important for spermatogenesis and embryo development (Kim et al. 2012) (Zhang et al. 2011). SMARCA4 is also the most frequently mutated chromatin remodeling ATPase in cancer. Mutations in the SMARCA4 have been observed in many tumor subtypes including pancreatic cancers and some types of leukemia (Jones et al. 2012) (Shain et al. 2012) (Hodges et al. 2018) (Romero et al. 2012).

Rat anti Human SMARCA4 antibody detects a band of 180 kDa.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A recombinant protein corresponding to amino acids 213-295 of human SMARCA4
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 2 ug/ml 10 ug/ml
Western Blotting 0.5 ug/ml 2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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UniProt
P51532
Entrez Gene
SMARCA4
GO Terms
GO:0002039 p53 binding
GO:0003677 DNA binding
GO:0003714 transcription corepressor activity
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0006338 chromatin remodeling
GO:0007399 nervous system development
GO:0008094 DNA-dependent ATPase activity
GO:0042802 identical protein binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016514 SWI/SNF complex
GO:0016563 transcription activator activity
GO:0045749 negative regulation of S phase of mitotic cell cycle
GO:0047485 protein N-terminus binding
GO:0051091 positive regulation of transcription factor activity
GO:0070577 histone acetyl-lysine binding
GO:0071564 npBAF complex
GO:0071565 nBAF complex
GO:0071778 WINAC complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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