PTEN Tumor Suppressor Protein antibody | RM265

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Rabbit anti PTEN Tumor Suppressor Protein

Product Type
Monoclonal Antibody
Clone
RM265
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6312
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human PTEN tumor suppressor protein antibody recognizes phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, also known as MMAC1, TEP1. PTEN is a lipid and protein phosphatase that can suppress the PI3K/AKT signaling pathway, which plays roles in regulating cell metabolism, survival, proliferation, apoptosis, growth and migration. In this way, PTEN acts as a tumor suppressor, and loss of PTEN drives many human cancers. Mutations in PIK3CA, PTEN, and/or AKT1 are present in over 40% of uterine, lung, breast, prostate, brain, and head and neck cancers (Chang et al. 2019). PTEN also has important roles in immune signaling pathways, regulates immune cell development, and helps generate antiviral responses (Chen and Guo, 2017). In the nucleus, PTEN has also been found to mediate cellular proliferation and stablize the genome (Morotti et al. 2015).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the C-terminus of Human PTEN tumor suppressor protein

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P60484
Entrez Gene
PTEN
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000287 magnesium ion binding
GO:0001933 negative regulation of protein phosphorylation
GO:0006915 apoptosis
GO:0004438 phosphatidylinositol-3-phosphatase activity
GO:0005829 cytosol
GO:0004722 protein serine/threonine phosphatase activity
GO:0004725 protein tyrosine phosphatase activity
GO:0006470 protein dephosphorylation
GO:0008289 lipid binding
GO:0006917 induction of apoptosis
GO:0007417 central nervous system development
GO:0008138 protein tyrosine/serine/threonine phosphatase activity
GO:0008283 cell proliferation
GO:0009898 internal side of plasma membrane
GO:0010975 regulation of neuron projection development
GO:0016314 phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity
GO:0016605 PML body
GO:0050821 protein stabilization
GO:0019899 enzyme binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030165 PDZ domain binding
GO:0030336 negative regulation of cell migration
GO:0046855 inositol phosphate dephosphorylation
GO:0046856 phosphatidylinositol dephosphorylation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051091 positive regulation of transcription factor activity
GO:0051717 inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity
GO:0051800 phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity
GO:0051895 negative regulation of focal adhesion assembly
GO:0051898 negative regulation of protein kinase B signaling cascade
GO:0060070 canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of PTEN Tumor Suppressor Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/2000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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