Browsing Modes for Business Users

Prkra antibody | AB01/1C8

ISO 9001 Quality Assurance Manufacturing | Print
siRNA Validated
IP-MS Validated
Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PRKRA antibody (1/1,000, VMA00926) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to PRKRA (molecular weight 32 kDa)
Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of PRKRA from Jurkat whole cell lysate. The Input lane is 10% input; the PRKRA -IP lane is the immunoprecipitation with the PRKRA antibody (2.5 µg, VMA00926); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA691 ). Western blot analysis was performed using PRKRA antibody (1/1000, VMA00926) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to PRKRA.
Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PRKRA (1 & 2) or a scrambled siRNA (-) probed with Mouse anti PRKRA antibody (VMA00926) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 49 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti Prkra Antibody, clone AB01/1C8 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of PRKRA from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PRKRA (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Mouse anti Prkra

Product Type
PrecisionAb Monoclonal
Clone
AB01/1C8
Isotype
IgG2b
Format
Purified
Specificity
Prkra

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
VMA00926
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
List Price Your Price
loader

Mouse anti PRKRA antibody recognizes interferon-inducible double-stranded RNA-dependent protein kinase activator A, also known as PACT and RAX.

PRKRA is a double-stranded RNA binding protein that plays a role in the innate immune response to viral infection (Iwamura et al. 2001). In the host-influenza A interaction, the antiviral activity of PRKRA comes from its ability to inhibit viral polymerase (Chan et al. 2018). A specific mutation in PRKRA is a risk factor for dystonia (Camargos et al. 2008). PRKRA activates PKR (Patel and Sen 1998), and PKR modulation has been suggested as an antitumor strategy due to its effects on apoptosis and proliferation (Xu et al. 2012). 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti PRKRA detects a band of approximately 32 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-313 of human PRKRA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
O75569
Entrez Gene
PRKRA
GO Terms
GO:0003725 double-stranded RNA binding
GO:0009615 response to virus
GO:0006955 immune response
GO:0008047 enzyme activator activity
GO:0008285 negative regulation of cell proliferation
GO:0030422 production of siRNA involved in RNA interference
GO:0042803 protein homodimerization activity
GO:0048471 perinuclear region of cytoplasm

VMA00926

100005647

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note