Prkra antibody | AB01/1C8

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Mouse anti Prkra

Product Type
PrecisionAb Monoclonal
Clone
AB01/1C8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00926
Datasheet
IP WB
SDS
100 µl loader

Mouse anti PRKRA antibody recognizes interferon-inducible double-stranded RNA-dependent protein kinase activator A, also known as PACT and RAX.

PRKRA is a double-stranded RNA binding protein that plays a role in the innate immune response to viral infection (Iwamura et al. 2001). In the host-influenza A interaction, the antiviral activity of PRKRA comes from its ability to inhibit viral polymerase (Chan et al. 2018). A specific mutation in PRKRA is a risk factor for dystonia (Camargos et al. 2008). PRKRA activates PKR (Patel and Sen 1998), and PKR modulation has been suggested as an antitumor strategy due to its effects on apoptosis and proliferation (Xu et al. 2012).

Product Details

Target Species
Human
Western Blotting
Mouse anti PRKRA detects a band of approximately 32 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-313 of human PRKRA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O75569
Entrez Gene
PRKRA
GO Terms
GO:0003725 double-stranded RNA binding
GO:0009615 response to virus
GO:0006955 immune response
GO:0008047 enzyme activator activity
GO:0008285 negative regulation of cell proliferation
GO:0030422 production of siRNA involved in RNA interference
GO:0042803 protein homodimerization activity
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Prkra antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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