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MAPK14 antibody | CPTC24

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Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with MAPK14 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 20.91 second exposure. Arrow points to MAPK14 (molecular weight 41 kDa)
Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of MAPK14 CRISPR knockout HEK293 (MAPK14 KO) and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti MAPK14 antibody (VMA00352) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 11 second and (B) 30 second exposure.
Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of MAPK14 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 4
Figure A. RPE conjugated mouse anti human CD3 (MCA2184PE) and Mouse anti mouse IgG1 isotype control (MCA928) detected with FITC conjugated goat anti mouse IgG1 (STAR132F). Figure B. RPE conjugated mouse anti human CD3 (MCA2184PE) and Mouse anti MAPK14 (VMA00352) detected with FITC conjugated goat anti mouse IgG1 (STAR132F). All experiments performed on Leucoperm (BUF09) fixed and permeabilized Jurkat cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti MAPK14

Product Type
PrecisionAb Monoclonal
Clone
CPTC24
Isotype
IgG1
Format
Purified
Specificity
MAPK14

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Product Code Applications Pack Size List Price Your Price Qty
VMA00352
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F WB 100 µl loader
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Mouse anti Human MAPK14 antibody recognizes mitogen-activated protein kinase 14, also known as CSAID-binding protein, Csaids binding protein, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha, MAX-interacting protein 2, cytokine suppressive anti-inflammatory drug binding protein, mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip and stress-activated protein kinase 2A.

Encoded by the MAPK14 gene, mitogen-activated protein kinase 14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of MAPK14 encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human Mitogen-activated protein kinase 14 antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MAPK14 detects a band of approximately 41 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human MAPK14
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q16539
Entrez Gene
MAPK14
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004707 MAP kinase activity
GO:0004708 MAP kinase kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006928 cellular component movement
GO:0006935 chemotaxis
GO:0007265 Ras protein signal transduction
GO:0008063 Toll signaling pathway
GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
GO:0016071 mRNA metabolic process
GO:0030168 platelet activation
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042692 muscle cell differentiation
GO:0042770 signal transduction in response to DNA damage
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051149 positive regulation of muscle cell differentiation
GO:0051403 stress-activated MAPK cascade
GO:0071479 cellular response to ionizing radiation
GO:0090400 stress-induced premature senescence

VMA00352

151215

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