MAPK14 antibody | CPTC24

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Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MAPK14 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 20.91 second exposure. Arrow points to MAPK14 (molecular weight 41 kDa)

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of MAPK14 CRISPR knockout HEK293 (MAPK14 KO) and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti MAPK14 antibody (**VMA00352**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 11 second and (B) 30 second exposure.

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of MAPK14 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti MAPK14 Antibody, clone CPTC24 (PrecisionAb Monoclonal Antibody) gallery image 4 — Figure A. RPE conjugated mouse anti human CD3 (MCA2184PE) and Mouse anti mouse IgG1 isotype control (MCA928) detected with FITC conjugated goat anti mouse IgG1 (STAR132F). Figure B. RPE conjugated mouse anti human CD3 (MCA2184PE) and Mouse anti MAPK14 (VMA00352) detected with FITC conjugated goat anti mouse IgG1 (STAR132F). All experiments performed on Leucoperm (BUF09) fixed and permeabilized Jurkat cells gated on live single cells. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti MAPK14

Product Type: PrecisionAb Monoclonal
Clone: CPTC24
Isotype: IgG1
Format: Purified
Specificity: MAPK14

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00352 Datasheet Datasheet SDS SDS	F WB	100 µl		Log in	Get Quote
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Mouse anti Human MAPK14 antibody recognizes mitogen-activated protein kinase 14, also known as CSAID-binding protein, Csaids binding protein, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha, MAX-interacting protein 2, cytokine suppressive anti-inflammatory drug binding protein, mitogen-activated protein kinase p38 alpha, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip and stress-activated protein kinase 2A.

Encoded by the MAPK14 gene, mitogen-activated protein kinase 14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of MAPK14 encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human Mitogen-activated protein kinase 14 antibody detects a band of 41 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MAPK14 detects a band of approximately 41 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human MAPK14
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q16539
Entrez Gene: MAPK14
GO Terms: GO:0000187 activation of MAPK activity; GO:0005515 protein binding; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004707 MAP kinase activity; GO:0004708 MAP kinase kinase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; View More GO Terms; GO:0006928 cellular component movement; GO:0006935 chemotaxis; GO:0007265 Ras protein signal transduction; GO:0008063 Toll signaling pathway; GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process; GO:0016071 mRNA metabolic process; GO:0030168 platelet activation; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0042692 muscle cell differentiation; GO:0042770 signal transduction in response to DNA damage; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051090 regulation of transcription factor activity; GO:0051149 positive regulation of muscle cell differentiation; GO:0051403 stress-activated MAPK cascade; GO:0071479 cellular response to ionizing radiation; GO:0090400 stress-induced premature senescence