IL-4 antibody | MP4-25D2

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1		1/10	1/50
Immunohistology - Frozen
Western Blotting		1/500	1/1000

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

References for IL-4 antibody

1. Krohn, A.J. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector.
   J Neurosci. 18 (20): 8186-97.
2. Kubota, M. et al. (1997) Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates.
   J Immunol. 158 (11): 5321-9.
3. Patterson, B.K. et al. (2001) Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women.
   J Clin Invest. 107 (3): 287-94.
4. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.
5. Carvalho, E.M. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
   J Immunol. 152 (12): 5949-56.
6. P, M. et al. (2004) Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
   J Immunol. 172 (12): 7341-9.
7. Raulf-Heimsoth, M. (2008) T cell - primary culture from peripheral blood.
   Methods Mol Med. 138: 17-30.

RRID

AB_567085

UniProt

P05112

Entrez Gene

IL4

GO Terms

GO:0005125 cytokine activity

GO:0005136 interleukin-4 receptor binding

GO:0005615 extracellular space

GO:0006935 chemotaxis

GO:0006968 cellular defense response

GO:0008083 growth factor activity

GO:0008203 cholesterol metabolic process

GO:0016563 transcription activator activity

GO:0016564 transcription repressor activity

View More GO Terms

GO:0030183 B cell differentiation

GO:0030890 positive regulation of B cell proliferation

GO:0042092 type 2 immune response

GO:0043066 negative regulation of apoptosis

GO:0045189 connective tissue growth factor biosynthetic process

GO:0045348 positive regulation of MHC class II biosynthetic process

GO:0045671 negative regulation of osteoclast differentiation

GO:0045944 positive regulation of transcription from RNA polymerase II promoter

GO:0048295 positive regulation of isotype switching to IgE isotypes

GO:0048304 positive regulation of isotype switching to IgG isotypes

MCA1525

0611 0611R 163723

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