IL-4 antibody | MP4-25D2

Rat anti Human Interleukin-4

Product Type
Monoclonal Antibody
Clone
MP4-25D2
Isotype
IgG1
Specificity
IL-4

Product Code Applications Pack Size List Price Your Price Qty
MCA1525
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SDS Safety Datasheet SDS
C E F * WB 0.5 mg loader
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Rat anti Human Interleukin-4 antibody, clone MP4-25D2 recognizes human Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, Binetrakin, Pitrakinra or Lymphocyte stimulatory factor 1. Interleukin-4 is a 153 amino acid ~17kDa cytokine involved in the activation of B cells, macrophages and monocytes (UniProt: P05112).

Polymorphisms in the IL-4 gene are associated with susceptibility to, and outcome of ischemic stroke (Marousi et al. 2011).

Rat anti Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity (Abrams et al. 1992 and Carvalho et al. 1994).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified CHO cell expressed recombinant human IL-4.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/50
Immunohistology - Frozen
Western Blotting 1/500 1/1000
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for IL-4 antibody

  1. Krohn, A.J. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector.
    J Neurosci. 18 (20): 8186-97.
  2. Kubota, M. et al. (1997) Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates.
    J Immunol. 158 (11): 5321-9.
  3. Patterson, B.K. et al. (2001) Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women.
    J Clin Invest. 107 (3): 287-94.
  4. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  5. Carvalho, E.M. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
    J Immunol. 152 (12): 5949-56.
  6. P, M. et al. (2004) Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
    J Immunol. 172 (12): 7341-9.
  7. Raulf-Heimsoth, M. (2008) T cell - primary culture from peripheral blood.
    Methods Mol Med. 138: 17-30.

RRID
AB_567085
UniProt
P05112
Entrez Gene
IL4
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006968 cellular defense response
GO:0008083 growth factor activity
GO:0008203 cholesterol metabolic process
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0042092 type 2 immune response
GO:0043066 negative regulation of apoptosis
GO:0045189 connective tissue growth factor biosynthetic process
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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