Rat anti Human Interleukin-4 antibody, clone MP4-25D2
recognizes human Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, Binetrakin, Pitrakinra or Lymphocyte stimulatory factor 1. Interleukin-4 is a 153 amino acid ~17kDa cytokine involved in the activation of B cells, macrophages and monocytes (UniProt: P05112).
Polymorphisms in the IL-4 gene are associated with susceptibility to, and outcome of ischemic stroke (Marousi et al. 2011).
Rat anti Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity (Abrams et al. 1992 and Carvalho et al. 1994).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Purified CHO cell expressed recombinant human IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
interleukin-4 receptor binding
cellular defense response
growth factor activity
cholesterol metabolic process
transcription activator activity
transcription repressor activity
B cell differentiation
positive regulation of B cell proliferation
type 2 immune response
negative regulation of apoptosis
connective tissue growth factor biosynthetic process
positive regulation of MHC class II biosynthetic process
negative regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-4 antibody
|Flow Cytometry 1
|Immunohistology - Frozen
- 1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for IL-4 antibody
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Carvalho, E.M. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
J Immunol. 152 (12): 5949-56.
P, M. et al. (2004) Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
J Immunol. 172 (12): 7341-9.
Raulf-Heimsoth, M. (2008) T cell - primary culture from peripheral blood.
Methods Mol Med. 138: 17-30.
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