Browsing Modes for Business Users

IL-4 antibody | MP4-25D2

ISO 9001 Quality Assurance Manufacturing | Print
Anti Human Interleukin-4 Antibody, clone MP4-25D2 thumbnail image 1
Anti Human Interleukin-4 Antibody, clone MP4-25D2 gallery image 1
Western blot analysis of human IL-4 recombinant protein probed with Rat anti Human Interleukin-4 (MCA1525) followed by F(ab')2 Rabbit anti Rat IgG:HRP (STAR21B).

Rat anti Human Interleukin-4:RPE

Product Type
Monoclonal Antibody
Clone
MP4-25D2
Isotype
IgG1
Specificity
IL-4

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA1525PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests loader
List Price Your Price
loader

Rat anti Human Interleukin-4 antibody, clone MP4-25D2 recognizes human Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, Binetrakin, Pitrakinra or Lymphocyte stimulatory factor 1. Interleukin-4 is a 153 amino acid ~17kDa cytokine involved in the activation of B cells, macrophages and monocytes (UniProt: P05112).

Polymorphisms in the IL-4 gene are associated with susceptibility to, and outcome of ischemic stroke (Marousi et al. 2011).

Rat anti Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity (Abrams et al. 1992 and Carvalho et al. 1994).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Purified CHO cell expressed recombinant human IL-4.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 590 620
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG1 Negative Control:RPE MCA6004PE F 100 Tests
>
List Price Your Price
Description Rat IgG1 Negative Control:RPE

References for IL-4 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  2. Carvalho, E.M. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
    J Immunol. 152 (12): 5949-56.
  3. P, M. et al. (2004) Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
    J Immunol. 172 (12): 7341-9.
  4. Raulf-Heimsoth, M. (2008) T cell - primary culture from peripheral blood.
    Methods Mol Med. 138: 17-30.

UniProt
P05112
Entrez Gene
IL4
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006968 cellular defense response
GO:0008083 growth factor activity
GO:0008203 cholesterol metabolic process
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0042092 type 2 immune response
GO:0043066 negative regulation of apoptosis
GO:0045189 connective tissue growth factor biosynthetic process
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
View more products with IL-4 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note