IL-10 antibody | JES3-9D7

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Rat anti Human Interleukin 10:Low Endotoxin

Rat anti Human Interleukin-10

Rat anti Human Interleukin-10:RPE

Product Type
Monoclonal Antibody
Clone
JES3-9D7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1531EL E F* FN WB 0.5 mg
MCA1531 E F* WB 0.5 mg
MCA1531PE F* 100 Tests
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 recognizes human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.

Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
NonePresent
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant human IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P22301 Related reagents
Entrez Gene
IL10 Related reagents
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0030183 B cell differentiation
GO:0030595 leukocyte chemotaxis
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042092 type 2 immune response
GO:0042100 B cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043193 positive regulation of gene-specific transcription
GO:0045191 regulation of isotype switching
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
Regulatory
For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/50
Functional Assays
Western Blotting 1/100
ELISA
Flow Cytometry 1 1/10 1/50
Western Blotting 1/100
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:Low Endotoxin MCA6004EL 0.5 mg F
Rat IgG1 Negative Control MCA6004GA 0.1 mg F
Rat IgG1 Negative Control:RPE MCA6004PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Functional Assays

Product Specific References

References for IL-10 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  2. Othieno, C. et al. (1999) Interaction of Mycobacterium tuberculosis-induced transforming growth factor beta1 and interleukin-10.
    Infect Immun. 67 (11): 5730-5.
  3. Benjamin, J.L. et al. (2011) Randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active Crohn's diseas
    Gut gut.2010.232025
  4. Cua, D.J. et al. (1999) Transgenic interleukin 10 prevents induction of experimental autoimmune encephalomyelitis.
    J Exp Med. 189: 1005-10.
  5. Lindmark, E. & Siegbahn, A. (2002) Tissue factor regulation and cytokine expression in monocyte-endothelial cell co-cultures: effects of a statin, an ACE-inhibitor and a low-molecular-weight heparin.
    Thromb Res. 108 (1): 77-84.