IL-10 antibody | JES3-9D7

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1		1/10	1/50
Western Blotting			1/100

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

References for IL-10 antibody

1. Othieno, C. et al. (1999) Interaction of Mycobacterium tuberculosis-induced transforming growth factor beta1 and interleukin-10.
   Infect Immun. 67 (11): 5730-5.
2. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.
3. Lindmark, E. & Siegbahn, A. (2002) Tissue factor regulation and cytokine expression in monocyte-endothelial cell co-cultures: effects of a statin, an ACE-inhibitor and a low-molecular-weight heparin.
   Thromb Res. 108 (1): 77-84.
4. Benjamin, J.L. et al. (2011) Randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active Crohn's disease.
   Gut. 60 (7): 923-9.
5. Cua, D.J. et al. (1999) Transgenic interleukin 10 prevents induction of experimental autoimmune encephalomyelitis.
   J Exp Med. 189: 1005-10.

Functional Assays

RRID

AB_567068

UniProt

P22301

Entrez Gene

IL10

GO Terms

GO:0002740 negative regulation of cytokine secretion involved in immune response

GO:0002904 positive regulation of B cell apoptosis

GO:0005125 cytokine activity

GO:0005141 interleukin-10 receptor binding

GO:0005615 extracellular space

GO:0006916 anti-apoptosis

GO:0006954 inflammatory response

GO:0007253 cytoplasmic sequestering of NF-kappaB

GO:0007267 cell-cell signaling

View More GO Terms

GO:0008083 growth factor activity

GO:0030183 B cell differentiation

GO:0030595 leukocyte chemotaxis

GO:0030889 negative regulation of B cell proliferation

GO:0032715 negative regulation of interleukin-6 production

GO:0032800 receptor biosynthetic process

GO:0042092 type 2 immune response

GO:0042100 B cell proliferation

GO:0042130 negative regulation of T cell proliferation

GO:0043193 positive regulation of gene-specific transcription

GO:0045191 regulation of isotype switching

GO:0045347 negative regulation of MHC class II biosynthetic process

GO:0045355 negative regulation of interferon-alpha biosynthetic process

GO:0050715 positive regulation of cytokine secretion

GO:0051045 negative regulation of membrane protein ectodomain proteolysis

GO:0051091 positive regulation of transcription factor activity

GO:0051384 response to glucocorticoid stimulus

MCA1531

1601

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