IL-10 antibody | JES3-9D7

Rat anti Human Interleukin-10

Product Type
Monoclonal Antibody
Clone
JES3-9D7
Isotype
IgG1
Specificity
IL-10

Product Code Applications Pack Size List Price Your Price Qty
MCA1531
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * WB 0.5 mg loader
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loader
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Rat anti Human Interleukin-10 antibody, clone JES3-9D7 recognizes human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.

Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant human IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/50
Western Blotting 1/100
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for IL-10 antibody

  1. Othieno, C. et al. (1999) Interaction of Mycobacterium tuberculosis-induced transforming growth factor beta1 and interleukin-10.
    Infect Immun. 67 (11): 5730-5.
  2. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  3. Lindmark, E. & Siegbahn, A. (2002) Tissue factor regulation and cytokine expression in monocyte-endothelial cell co-cultures: effects of a statin, an ACE-inhibitor and a low-molecular-weight heparin.
    Thromb Res. 108 (1): 77-84.
  4. Benjamin, J.L. et al. (2011) Randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active Crohn's disease.
    Gut. 60 (7): 923-9.
  5. Cua, D.J. et al. (1999) Transgenic interleukin 10 prevents induction of experimental autoimmune encephalomyelitis.
    J Exp Med. 189: 1005-10.

Functional Assays

RRID
AB_567068
UniProt
P22301
Entrez Gene
IL10
GO Terms
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005141 interleukin-10 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0030183 B cell differentiation
GO:0030595 leukocyte chemotaxis
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042092 type 2 immune response
GO:0042100 B cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043193 positive regulation of gene-specific transcription
GO:0045191 regulation of isotype switching
GO:0045347 negative regulation of MHC class II biosynthetic process
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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