IL-10 antibody | JES3-9D7
Rat anti Human Interleukin 10:Low Endotoxin
Rat anti Human Interleukin-10
Rat anti Human Interleukin-10:RPE
- Product Type
- Monoclonal Antibody
- Clone
- JES3-9D7
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1531EL | E F* FN WB | 0.5 mg |
|
|
| MCA1531 | E F* WB | 0.5 mg |
|
|
| MCA1531PE | F* | 100 Tests |
|
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 recognizes human Interleukin - 10 (IL-10). IL-10 is an immunoregulatory cytokine which is expressed by activated CD8+ve peripheral blood T-cells, CD4+ve T-cell clones, B cell lymphomas, activated monocytes and mast cells.
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).
Rat anti Human Interleukin-10 antibody, clone JES3-9D7 is reported to act as a neutralising antibody (Othieno et al. 1999).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
None Present - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-10.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P22301
- Entrez Gene
- IL10
- GO Terms
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0002904 positive regulation of B cell apoptosis
- GO:0005125 cytokine activity
- GO:0005141 interleukin-10 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0007253 cytoplasmic sequestering of NF-kappaB
- GO:0007267 cell-cell signaling
- GO:0008083 growth factor activity
- GO:0030183 B cell differentiation
- GO:0030595 leukocyte chemotaxis
- GO:0030889 negative regulation of B cell proliferation
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032800 receptor biosynthetic process
- GO:0042092 type 2 immune response
- GO:0042100 B cell proliferation
- GO:0042130 negative regulation of T cell proliferation
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045191 regulation of isotype switching
- GO:0045347 negative regulation of MHC class II biosynthetic process
- GO:0045355 negative regulation of interferon-alpha biosynthetic process
- GO:0050715 positive regulation of cytokine secretion
- GO:0051045 negative regulation of membrane protein ectodomain proteolysis
- GO:0051091 positive regulation of transcription factor activity
- GO:0051384 response to glucocorticoid stimulus
- Regulatory
- For research purposes only
Applications of IL-10 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Functional Assays | |||
| Western Blotting | 1/100 | ||
| ELISA | |||
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Western Blotting | 1/100 | ||
| Flow Cytometry 1 | Neat |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG1 Negative Control:Low Endotoxin | MCA6004EL | F | 0.5 mg |
|
|
| Rat IgG1 Negative Control | MCA6004GA | F | 0.1 mg |
|
|
| Rat IgG1 Negative Control:RPE | MCA6004PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for IL-10 antibody
-
Othieno, C. et al. (1999) Interaction of Mycobacterium tuberculosis-induced transforming growth factor beta1 and interleukin-10.
Infect Immun. 67 (11): 5730-5. -
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24. -
Lindmark, E. & Siegbahn, A. (2002) Tissue factor regulation and cytokine expression in monocyte-endothelial cell co-cultures: effects of a statin, an ACE-inhibitor and a low-molecular-weight heparin.
Thromb Res. 108 (1): 77-84. -
Benjamin, J.L. et al. (2011) Randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active Crohn's disease.
Gut. 60 (7): 923-9. -
Cua, D.J. et al. (1999) Transgenic interleukin 10 prevents induction of experimental autoimmune encephalomyelitis.
J Exp Med. 189: 1005-10.
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