HLA DR antibody | YD1/63.4.10
Rat anti Human HLA-DR antibody, clone YD1/63.4.10 used for the evaluation of MHC class II expression on human PBMC by flow cytometry.
Image caption:
Plasmacytoid dendritic cells show weak T cell stimulatory capacity. A: Flow cytometric analysis of human PBMC and DC. pDC are characterized as lineage− CD11c− CD303+ cells (middle panel). Dot plots (right side) exemplarily depicts isolated pDC, percentage indicates purity. Histograms show surface expression of CD40, CD80, CD86, CD58 and HLA-DR on resting and CpG/IL-3-activated pDC and cytokine cocktail activated cDC. Isotype-matched control mAb staining is shown in grey. One representative result of three independent experiments is shown.
From: Hubo M, Jonuleit H (2012) Plasmacytoid Dendritic Cells Are Inefficient in Activation of Human Regulatory T Cells.
PLoS ONE 7(8): e44056.
Rat anti Human HLA DR
- Product Type
- Monoclonal Antibody
- Clone
- YD1/63.4.10
- Isotype
- IgG2a
- Specificity
- HLA DR
Antibody Quality and Performance Guarantee
| Rat anti Human HLA DR antibody, clone YD1/63.4.10 recognizes a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- DAUDI cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells of immunized DA rats were fused with cells of the Y3 Ag.1.2.3 Rat myeloma line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
- Histology Positive Control Tissue
- Tonsil
References for HLA DR antibody
-
Hubo, M. & Jonuleit, H. (2012) Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells.
PLoS One. 7 (8): e44056. -
Janossy, G. et al. (1980) Standardization of monoclonal antibodies for clinical studies in leukaemia/lymphoma.
Protides Biol Fluid Proc Colloq. 28:523-527. -
Pawelec, G. et al. (1982) Long-term culture, cloning, and surface markers of mixed leukocyte culture-derived human T lymphocytes with natural killer-like cytotoxicity.
J Immunol. 128(4):1892-1896.
- RRID
- AB_567320
MCA72G
150853
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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