HLA DR antibody | YD1/63.4.10

Rat anti Human HLA DR

Product Type
Monoclonal Antibody
Clone
YD1/63.4.10
Isotype
IgG2a
Specificity
HLA DR

Product Code Applications Pack Size List Price Your Price Qty
MCA72G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Human HLA DR antibody, clone YD1/63.4.10 recognizes a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
DAUDI cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells of immunised DA rats were fused with cells of the Y3 Ag.1.2.3 Rat myeloma line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Histology Positive Control Tissue
Tonsil

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References for HLA DR antibody

  1. Hubo, M. & Jonuleit, H. (2012) Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells.
    PLoS One. 7 (8): e44056.
  2. Janossy, G. et al. (1980) Standardization of monoclonal antibodies for clinical studies in leukaemia/lymphoma.
    Protides Biol Fluid Proc Colloq. 28:523-527.
  3. Pawelec, G. et al. (1982) Long-term culture, cloning, and surface markers of mixed leukocyte culture-derived human T lymphocytes with natural killer-like cytotoxicity.
    J Immunol. 128(4):1892-1896.

RRID
AB_567320
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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