Histone H3 (pSer10) antibody | 6G8B7
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|Rat anti Human histone H3 (pSer10) antibody, clone 6G8B7 recognizes histone H3 when phosphorylated at serine 10.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Phosphorylation of histone H3 has been shown to occur during the early stages of mitosis when chromosomes begin to condense, and during premature condensing of chromosomes during S-phase of the cell cycle. Phosphorylation of histone H3 at serine 10 has been linked to both transcription and cell division (Hans and Dimitrov, 2001).
Broad species cross-reactivity is expected from Rat anti Human histone H3 (pSer10) antibody based on sequence.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Broad Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
- Carrier Free
- A peptide corresponding to amino acids 1-19 including phosphorylated serine 10 of human histone H3
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunofluorescence||0.5 ug/ml||2 ug/ml|
|Western Blotting||0.2 ug/ml||2 ug/ml|
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