Histone H3 (Ac9) antibody | 2G1F9

Rat anti Human Histone H3 (Ac9)

Product Type
Monoclonal Antibody
Clone
2G1F9
Isotype
IgG2a
Specificity
Histone H3 (Ac9)

Product Code Applications Pack Size List Price Your Price Qty
MCA6379
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch IF WB 0.1 mg loader
List Price Your Price
loader
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Rat anti Human histone H3 (Ac9) antibody, clone 2G1F9 recognizes histone H3 when acetylated at lysine 9.

Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions (Kuo and Allis, 1998). Acetylation of histone H3 at lysine 4 usually leads to the transcriptional activation of genes (Hazzlin and Mahadevan, 2005).

Wide species cross-reactivity is expected from Rat anti Human histone H3 (Ac9) antibody based on sequence.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Broad Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide containing acetyl-lysine 9 of human histone H3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 5 ug per ChIP 10 ug per ChIP
Immunofluorescence 1 ug/ml 2 ug/ml
Western Blotting 0.5 ug/ml 2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P68431
Entrez Gene
HIST1H3F
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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