Histone H3.1 (pSer28) antibody | 5D10D4
Rat anti Human Histone H3.1 (pSer28)
- Product Type
- Monoclonal Antibody
- Clone
- 5D10D4
- Isotype
- IgG2b
- Specificity
- Histone H3.1 (pSer28)
Antibody Quality and Performance Guarantee
| Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 recognizes Histone H3.1 when phosphorylated at serine 28. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and histone H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle (Tagami et al. 2004). Phosphorylation of histone H3.1 at serine 28 occurs in early mitosis and during premature chromosome condensation in cells that are in S-phase of the cell cycle (Zhong et al. 2003). Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 does not react with histone H3.3 phosphorylated on serine 28. Wide species cross-reactivity is expected based on sequence. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Broad Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A peptide containing phosphorylated serine 28 of human histone H3.1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
2 ug/ml | 10 ug/ml |
| Western Blotting | |
0.5 ug/ml | 2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
MCA6385
160849
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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