Endothelin antibody | 3G10

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Rat anti Human Endothelin

Product Type
Monoclonal Antibody
Clone
3G10
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4113-0957 C* IR 0.1 mg
Rat anti Human endothelin antibody, clone 3G10 reacts with the C terminal of endothelins 1, 2 and 3. Rat anti Human endothelin antibody, clone 3G10 does not react with the endothelin precursor molecule, big endothelin. The endothelins are potent vasoconstrictors and critical components in the progression of pulmonary arterial hypertension a devastating and rapidly progressive condition that leads to right ventricular strain and dysfunction.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the c-terminal region of human endothelin (CHLDIIW) conjugated to BSA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05305 Related reagents
P14138 Related reagents
P20800 Related reagents
Entrez Gene
EDN1 Related reagents
EDN3 Related reagents
EDN2 Related reagents
GO Terms
GO:0001516 prostaglandin biosynthetic process
GO:0003100 regulation of systemic arterial blood pressure by endothelin
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0019722 calcium-mediated signaling
GO:0010460 positive regulation of heart rate
GO:0010595 positive regulation of endothelial cell migration
GO:0010613 positive regulation of cardiac muscle hypertrophy
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0014824 artery smooth muscle contraction
GO:0014826 vein smooth muscle contraction
GO:0046887 positive regulation of hormone secretion
GO:0019229 regulation of vasoconstriction
GO:0030072 peptide hormone secretion
GO:0030185 nitric oxide transport
GO:0030195 negative regulation of blood coagulation
GO:0031707 endothelin A receptor binding
GO:0031708 endothelin B receptor binding
GO:0042313 protein kinase C deactivation
GO:0043507 positive regulation of JUN kinase activity
GO:0045321 leukocyte activation
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045793 positive regulation of cell size
GO:0045840 positive regulation of mitosis
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0051771 negative regulation of nitric-oxide synthase biosynthetic process
GO:0060298 positive regulation of sarcomere organization
GO:0060585 positive regulation of prostaglandin-endoperoxide synthase activity
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0005625 soluble fraction
GO:0007166 cell surface receptor linked signaling pathway
GO:0048016 inositol phosphate-mediated signaling
GO:0030593 neutrophil chemotaxis
GO:0043406 positive regulation of MAP kinase activity
GO:0003058 hormonal regulation of the force of heart contraction
GO:0003099 positive regulation of the force of heart contraction by chemical signal
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0008284 positive regulation of cell proliferation
GO:0019221 cytokine-mediated signaling pathway
GO:0042116 macrophage activation
GO:0048246 macrophage chemotaxis
Regulatory
For research purposes only

Applications of Endothelin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1
IRMA
  1. 1Frozen sections processed with dry acetone are recommended. Paraformaldehyde is not recommended as it destroys the epitope.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Frozen sections processed with dry acetone are recommended. Paraformaldehyde is not recommended as it destroys the epitope.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for Endothelin antibody

  1. Yao, J. et al. (2001) Endothelin is a potent inhibitor of matrix metalloproteinase-2 secretion and activation in rat mesangial cells.
    Am J Physiol Renal Physiol. 280: F628-635.