Rat anti Human endothelin antibody, clone 3G10 reacts with the C terminal of endothelins 1, 2 and 3. Rat anti Human endothelin antibody, clone 3G10 does not react with the endothelin precursor molecule, big endothelin. The endothelins are potent vasoconstrictors and critical components in the progression of pulmonary arterial hypertension a devastating and rapidly progressive condition that leads to right ventricular strain and dysfunction.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide corresponding to the c-terminal region of human endothelin (CHLDIIW) conjugated to BSA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
prostaglandin biosynthetic process
regulation of systemic arterial blood pressure by endothelin
positive regulation of heart rate
positive regulation of endothelial cell migration
positive regulation of cardiac muscle hypertrophy
phosphatidylinositol 3-kinase cascade
artery smooth muscle contraction
vein smooth muscle contraction
positive regulation of hormone secretion
regulation of vasoconstriction
peptide hormone secretion
nitric oxide transport
negative regulation of blood coagulation
endothelin A receptor binding
endothelin B receptor binding
protein kinase C deactivation
positive regulation of JUN kinase activity
positive regulation of nitric oxide biosynthetic process
positive regulation of cell size
positive regulation of mitosis
positive regulation of smooth muscle cell proliferation
negative regulation of nitric-oxide synthase biosynthetic process
positive regulation of sarcomere organization
positive regulation of prostaglandin-endoperoxide synthase activity
positive regulation of leukocyte chemotaxis
cell surface receptor linked signaling pathway
inositol phosphate-mediated signaling
positive regulation of MAP kinase activity
hormonal regulation of the force of heart contraction
positive regulation of the force of heart contraction by chemical signal
elevation of cytosolic calcium ion concentration
positive regulation of cell proliferation
cytokine-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Endothelin antibody
|Immunohistology - Frozen 1
- 1Frozen sections processed with dry acetone are recommended. Paraformaldehyde is not recommended as it destroys the epitope.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Frozen sections processed with dry acetone are recommended. Paraformaldehyde is not recommended as it destroys the epitope.
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Secondary Antibodies Available
Product Specific References
References for Endothelin antibody
Yao, J. et al. (2001) Endothelin is a potent inhibitor of matrix metalloproteinase-2 secretion and
activation in rat mesangial cells.
Am J Physiol Renal Physiol. 280: F628-635.
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